Recombinant Human Protein kinase C-binding protein NELL1 (NELL1)

Code CSB-YP846105HU
MSDS
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Source Yeast
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Code CSB-EP846105HU
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Source E.coli
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Code CSB-EP846105HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP846105HU
MSDS
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Source Baculovirus
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Code CSB-MP846105HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NELL1
Uniprot No.
Alternative Names
FLJ45906; IDH3GL; NEL like 1 (chicken); NEL like protein 1; Nel related protein 1; NEL-like 1 (chicken); NEL-like protein 1; Nel-related protein 1; NELL 1 protein short isoform; Nell1; NELL1_HUMAN; Neural epidermal growth factor like 1; NRP1; Protein kinase C binding protein NELL1; Protein kinase C-binding protein NELL1
Species
Homo sapiens (Human)
Expression Region
22-810
Target Protein Sequence
FGMDPDLQM DIVTELDLVN TTLGVAQVSG MHNASKAFLF QDIEREIHAA PHVSEKLIQL FRNKSEFTIL ATVQQKPSTS GVILSIRELE HSYFELESSG LRDEIRYHYI HNGKPRTEAL PYRMADGQWH KVALSVSASH LLLHVDCNRI YERVIDPPDT NLPPGINLWL GQRNQKHGLF KGIIQDGKII FMPNGYITQC PNLNHTCPTC SDFLSLVQGI MDLQELLAKM TAKLNYAETR LSQLENCHCE KTCQVSGLLY RDQDSWVDGD HCRNCTCKSG AVECRRMSCP PLNCSPDSLP VHIAGQCCKV CRPKCIYGGK VLAEGQRILT KSCRECRGGV LVKITEMCPP LNCSEKDHIL PENQCCRVCR GHNFCAEGPK CGENSECKNW NTKATCECKS GYISVQGDSA YCEDIDECAA KMHYCHANTV CVNLPGLYRC DCVPGYIRVD DFSCTEHDEC GSGQHNCDEN AICTNTVQGH SCTCKPGYVG NGTICRAFCE EGCRYGGTCV APNKCVCPSG FTGSHCEKDI DECSEGIIEC HNHSRCVNLP GWYHCECRSG FHDDGTYSLS GESCIDIDEC ALRTHTCWND SACINLAGGF DCLCPSGPSC SGDCPHEGGL KHNGQVWTLK EDRCSVCSCK DGKIFCRRTA CDCQNPSADL FCCPECDTRV TSQCLDQNGH KLYRSGDNWT HSCQQCRCLE GEVDCWPLTC PNLSCEYTAI LEGECCPRCV SDPCLADNIT YDIRKTCLDS YGVSRLSGSV WTMAGSPCTT CKCKNGRVCC SVDFECLQNN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in the control of cell growth and differentiation. Promotes osteoblast cell differentiation and terminal mineralization.
Gene References into Functions
  1. Results show that NELL1 methylation level was 6.8% higher in renal cell cancer (RCC) tissues associated with advanced disease of distant metastases, and shorter RFS. Analyses suggested that NELL1 DNA methylation is a promising candidate prognostic epigenetic biomarker for more detailed analyses of RCC disease. PMID: 30272321
  2. This work indicates that NELL-1, HMGB1, and CCN2 might enhance bone defect healing via the recruitment of endogenous cells and induction of vascularization and act via different processes than BMP2. PMID: 28463604
  3. NELL1 variant is associated with tobacco- and HPV-mediated oral oncogenesis. PMID: 30091681
  4. Results demonstrate that NELL1 is differentially expressed in fusion-positive alveolar rhabdomyosarcoma (ARMS) and in embryonal rhabdomyosarcoma (ERMS) samples, and they show that this transcriptional difference partially depends on genomic DNA methylation. The study also found that high NELL1 levels are correlated with negative RMS prognostic factors and with poor tumor outcomes. PMID: 28380437
  5. lipoaspirate-derived hPSCs present a novel and abundant cell source of MSCs for cartilage regeneration, and the combinatorial application of NELL-1, TGF-beta3, and BMP-6 with hPSCs may remarkably enhance and accelerate cartilage repair. PMID: 26700847
  6. establish the feasibility of combining NELL-1 with BMP2 to improve clinical bone regeneration and provide mechanistic insight into canonical Wnt pathway activity during NELL-1 and BMP2 osteogenesis PMID: 26772960
  7. Data suggest that TSPN (N-terminal thrombospondin-1-like) domain of NELL1 exhibits major heparin-binding sites which may be involved in interaction of NELL1 with cell surface heparan sulfate proteoglycans. PMID: 26627376
  8. NELL-1 signaling activates Wnt/beta-catenin signaling. PMID: 26082355
  9. We also detected lower relative expression of Nell-1 by real-time PCR. Furthermore, immunohistochemical analyses revealed that Nell-1 staining was less intense in cancer tissue relative to normal tissue and that the tumor cells had spread to the muscle PMID: 26090379
  10. CpG islands in the NELL1 and NELL2 promoters are hypermethylated in renal cell carcinoma.NELL1 and NELL2 protein expression is downregulated in clear cell renal carcinoma. PMID: 25726761
  11. NELL-1 demonstrates diffuse and reliable expression in benign but not malignant bone-forming skeletal tumors PMID: 25791475
  12. NELL1 overexpression greatly enhanced the osteogenic differentiation and mineral synthesis of iPSC-MSCs on RGD-grafted CPC scaffold for the first time. PMID: 25220281
  13. Two single-nucleotide polymorphisms of the NELL1 gene may represent a novel mechanism underlying hydrochlorothiazide-induced adverse metabolic effects (meta-analysis). PMID: 23400010
  14. NELL1 is able to promote the osteogenic differentiation of periodontal ligament stem cells, which may be related to the downregulation of Msx2 expression. PMID: 22767336
  15. Activation of the JNK pathway is necessary to mediate terminal osteogenic different-iation of Saos-2 osteosarcoma cells by rhNELL-1. PMID: 22797704
  16. Data indicate that LvNELL1 infection promoted the osteogenic differentiation of hADSCs, and the effect was comparable with that of LvBMP2. PMID: 23017834
  17. SHH and NELL-1 directed signaling produced additive effects on the pro-osteogenic and antiadipogenic differentiation of adipose derived stem cells. PMID: 22264144
  18. Prediction of radiographic severity in Ankolysing Spondylitis based on clinical variables can be significantly improved by including SNPs at ADRB1 (rs1801253), NELL1 (rs8176785) and MHC (rs1634747, rs9270986, rs7451962 and rs241453) genes. PMID: 22495925
  19. These results identify one potential mechanism of action for rhNELL-1 induced osteogenesis and highlight a fundamental difference between NELL-1 and BMP-2 signaling. PMID: 22580275
  20. NELL-1 may produce functional cartilage with properties similar to native cartilage. PMID: 21902605
  21. Results suggest that Osterix is a direct transcriptional regulator with repressive effect on NELL-1 gene expression, contributing to a delicate balance of regulatory effects on NELL-1 transcription with Runx2. PMID: 21931789
  22. Nell-1 is a candidate growth factor able to induce pericyte osteogenic differentiation. PMID: 21615216
  23. the effects of NELL-1 on osteoblastic differentiation and proliferation are partly through binding to APR3 PMID: 21723284
  24. The osteogenic effects of NELL1 on femoral distraction osteogenesis, was investigated. PMID: 20959151
  25. a potent growth factor that is highly specific to the osteochondral lineage, and has demonstrated robust induction of bone in multiple in vivo models [review] PMID: 20647499
  26. Data indicate that NELL1 is a ubiquitous inflammatory bowel disease susceptibility locus. PMID: 17684544
  27. Runx2 directly binds to the osteoblast specific binding elements 2 elements and transactivates the human NELL-1 promoter. PMID: 17042739
  28. Promoter hypermethylation of NELL1 is a common, tissue-specific event in human EAC, occurs early during Barrett's-associated esophageal neoplastic progression, and is a potential biomarker of poor prognosis in early-stage EAC PMID: 17452981
  29. findings suggest that upon binding to a specific receptor NELL1 transduces an osteogenic signal through activation of certain Tyr-kinases associated with the Ras-MAPK cascade, and finally leads to the osteogenic differentiation PMID: 18082140
  30. Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18519826
  31. Transgenic mice overexpressing Nell-1 develop craniosynostosis. PMID: 12235118

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Subcellular Location
Cytoplasm. Nucleus envelope. Secreted. Note=Colocalizes with ATRAID on the nuclear envelope and the perinuclear region.
Database Links

HGNC: 7750

OMIM: 602319

KEGG: hsa:4745

STRING: 9606.ENSP00000349654

UniGene: Hs.657172

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