Human Pulmonary surfactant-associated protein A,SP-A ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Name: Human Pulmonary surfactant-associated protein A,SP-A ELISA Kit
Brand: CUSABIO
SKU: CSB-E08683h

Product Details

Target Name

Pulmonary surfactant-associated protein A1

Alternative Names

SFTPA1; COLEC4; PSAP; SFTP1; SFTPA; SFTPA1B; Pulmonary surfactant-associated protein A1; PSP-A; PSPA; SP-A; SP-A1; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; Collectin-4

Abbreviation

SFTPA1

Uniprot No.

Q8IWL2

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, urine

Detection Range

15.6 pg/mL-1000pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	80-92
1:200	Average %	97
	Range %	91-105
1:400	Average %	99
	Range %	92-110
1:800	Average %	93
	Range %	86-98

Recovery

The recovery of human SP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	97	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.420	2.418	2.419	2.318
500	2.008	1.999	2.004	1.903
250	1.582	1.555	1.569	1.468
125	1.248	1.211	1.230	1.129
62.5	0.709	0.689	0.699	0.598
31.25	0.472	0.436	0.454	0.353
15.6	0.290	0.269	0.280	0.179
0	0.104	0.098	0.101

Materials provided

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human SP-A antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled SP-A antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

Pulmonary surfactant-associated protein A1 (SFTPA1) is a key component of pulmonary surfactant, a complex mixture of lipids and proteins that reduces surface tension in the alveoli and supports normal respiratory function. This hydrophilic protein plays important roles in lung homeostasis, including surfactant metabolism regulation, innate immune defense against respiratory pathogens, and maintenance of alveolar stability. Type II pneumocytes and Clara cells in the lungs primarily produce SFTPA1. Expression levels often change in various pulmonary diseases, making it an important biomarker for respiratory research and clinical studies.

The Human Pulmonary surfactant-associated protein A,SP-A ELISA Kit (CSB-E08683h) provides quantitative measurement of SFTPA1 in human samples using a sandwich assay principle. This kit works with multiple sample types including serum, plasma, cell culture supernatants, and urine, with a detection range of 15.6 pg/mL to 1000 pg/mL and sensitivity of 3.9 pg/mL. The assay requires 50-100 μL sample volume, operates with detection at 450 nm wavelength, and can be completed within 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in clinical research studies to measure pulmonary surfactant-associated protein A levels in human serum samples. The applications span respiratory disease research and biomarker studies where SP-A serves as an indicator of lung function and pulmonary health status.

• Respiratory disease research: Quantification of SP-A levels in patient serum samples to study pulmonary conditions and lung-related pathologies
• Biomarker profiling: Measurement of SP-A alongside other pulmonary biomarkers to determine comprehensive respiratory health panels
• Clinical biomarker studies: Analysis of serum SP-A concentrations in patient populations for disease characterization and research applications
• Pulmonary health monitoring: Evaluation of surfactant protein levels as indicators of lung function and respiratory system status

Code	CSB-E08683h
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
Have Questions?	Leave a Message or Start an on-line Chat

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.