Human Pulmonary surfactant-associated protein D,SP-D ELISA Kit

Instructions
Code CSB-E11166h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name surfactant protein D
Alternative Names COLEC7, PSP-D, SFTP4, SP-D, collectin 7|pulmonary surfactant apoprotein|pulmonary surfactant-associated protein D|surfactant, pulmonary-associated protein D|surfactant-associated protein, pulmonary
Abbreviation SFTPD
Uniprot No. P35247
Species Homo sapiens (Human)
Sample Types serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
Detection Range 1.25 ng/mL-80 ng/mL
Sensitivity 0.31 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SP-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-94  
1:2 Average % 96  
Range % 91-100  
1:4 Average % 98  
Range % 92-108  
1:8 Average % 92  
Range % 86-97  
Recovery
The recovery of human SP-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-97  
EDTA plasma (n=4) 98 90-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
80 2.198 2.068 2.133 2.042  
40 1.457 1.378 1.418 1.327  
20 0.925 0.884 0.905 0.814  
10 0.600 0.578 0.589 0.498  
5 0.410 0.393 0.402 0.311  
2.5 0.255 0.251 0.253 0.162  
1.25 0.136 0.134 0.135 0.044  
0 0.092 0.090 0.091    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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Citations

Target Data

Function Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Subcellular Location Secreted, extracellular space, extracellular matrix, Secreted, extracellular space, surface film
Protein Families SFTPD family
Tissue Specificity Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes).
Database Links

HGNC: 10803

OMIM: 178635

KEGG: hsa:6441

STRING: 9606.ENSP00000361366

UniGene: Hs.253495

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