Human RING finger protein 207(RNF207) ELISA kit

Code CSB-EL019870HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
ring finger protein 207
Alternative Names
RNF207 ELISA Kit; C1orf188 ELISA Kit; RING finger protein 207 ELISA Kit
Abbreviation
RNF207
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RNF207 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %92-101
1:2Average %86
Range %81-90
1:4Average %93
Range %89-98
1:8Average %90
Range %85-94
Recovery
The recovery of human RNF207 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)10195-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.719 2.519 2.619 2.500
5002.172 2.072 2.122 2.003
2501.504 1.607 1.556 1.437
1250.996 1.061 1.029 0.910
62.50.653 0.623 0.638 0.519
31.20.362 0.380 0.371 0.252
15.60.252 0.261 0.257 0.138
00.123 0.115 0.119
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human RNF207 ELISA Kit was designed for the quantitative measurement of Human RNF207 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Function
(From Uniprot)
Plays a role in cardiac repolarization possibly by stabilizing membrane expression of the potassium channel KCNH2/HERG, or by assisting its synthesis, folding or export from the endoplasmic reticulum, in a heat shock protein-dependent manner.
Gene References into Functions
  1. Data indicate that plasma E3 Ligase Rnf207 (ring finger protein 207) level in acute myocardial infarction patients increased significantly compared with that in healthy people. PMID: 25599194
  2. RNF207 is an important regulator of action potential duration, likely via effects on HERG trafficking and localization in a heat shock protein-dependent manner. PMID: 25281747
  3. Meta-analysis and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19305408
  4. Genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19305409
Subcellular Location
Cytoplasm.
Database Links

HGNC: 32947

OMIM: 616923

KEGG: hsa:388591

STRING: 9606.ENSP00000367173

UniGene: Hs.716549

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