Human Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit

Code CSB-EL019549HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
regenerating islet-derived 3 gamma
Alternative Names
LPPM429 ELISA Kit; Pancreatitis-associated protein 1B ELISA Kit; Pancreatitis-associated protein IB ELISA Kit; PAP IB ELISA Kit; PAP-1B ELISA Kit; PAP1B ELISA Kit; PAPIB ELISA Kit; REG III ELISA Kit; Reg III-gamma ELISA Kit; REG-3-gamma ELISA Kit; REG3G ELISA Kit; REG3G_HUMAN ELISA Kit; Regenerating islet derived 3 gamma ELISA Kit; Regenerating islet-derived protein 3-gamma ELISA Kit; Regenerating islet-derived protein III-gamma ELISA Kit; UNQ429 ELISA Kit
Abbreviation
REG3G
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human REG3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %88-97
1:2Average %99
Range %95-107
1:4Average %104
Range %100-110
1:8Average %93
Range %86-98
Recovery
The recovery of human REG3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-99
EDTA plasma (n=4)9085-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.519 2.352 2.436 2.296
1001.923 1.856 1.890 1.750
501.301 1.254 1.278 1.138
250.719 0.721 0.720 0.580
12.50.415 0.387 0.401 0.261
6.250.324 0.307 0.316 0.176
3.120.215 0.229 0.222 0.082
00.142 0.137 0.140
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human REG3G ELISA Kit was designed for the quantitative measurement of Human REG3G protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

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Target Background

Function
(From Uniprot)
Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.
Gene References into Functions
  1. This gene encodes a secreted peptide that displays antimicrobial activity against Gram-positive bacteria. PMID: 16931762
  2. a model of CagA-directed REG3gamma expression in gastric epithelial cells via activation of the IL-11/gp130/STAT3 pathway PMID: 22312430
  3. REG III was identified and expressed predominantly in pancreas and testis, but not in small intestine PMID: 15556304
  4. PAP IB represents a good example of duplication and divergence, probably with the acquisition of new functions, thus participating in the evolution of the protein repertoire. PMID: 15777617
Subcellular Location
Secreted. Cytoplasm.
Tissue Specificity
Predominantly expressed in pancreas, where it may be restricted to exocrine pancreas. Moderate expression levels in testis and weak in heart, kidney and placenta.
Database Links

HGNC: 29595

OMIM: 609933

KEGG: hsa:130120

STRING: 9606.ENSP00000272324

UniGene: Hs.447084

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