Human S100 calcium binding protein A12/Calgranulin-C(S100A12) ELISA Kit

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Human S100 calcium binding protein A12/Calgranulin-C(S100A12) ELISA Kit

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Citations (21) Protocol MSDS

Code	CSB-E13095h
Size	$790
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Product Details
Citations (21)
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Alternative Names

CAAF1 ELISA Kit; CAGC ELISA Kit; Calcitermin ELISA Kit; Calcium-binding protein in amniotic fluid 1 ELISA Kit; Calgranulin C ELISA Kit; Calgranulin-C ELISA Kit; Calgranulin-related protein ELISA Kit; CGRP ELISA Kit; EN RAGE ELISA Kit; EN-RAGE ELISA Kit; ENRAGE ELISA Kit; Extracellular newly identified RAGE-binding protein ELISA Kit; migration inhibitory factor-related protein 6 ELISA Kit; MRP6 ELISA Kit; Neutrophil S100 protein ELISA Kit; p6 ELISA Kit; Protein S100 A12 ELISA Kit; S100 calcium binding protein A12 ELISA Kit; S100 calcium-binding protein A12 (calgranulin C) ELISA Kit; S100 calcium-binding protein A12 ELISA Kit; S100A12 ELISA Kit; S10AC_HUMAN ELISA Kit

Abbreviation

S100A12

Uniprot No.

P80511

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/ml-10 ng/ml

Sensitivity

0.039ng/ml.

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:20	Average %	104
	Range %	99-109
1:40	Average %	112
	Range %	108-118
1:80	Average %	98
	Range %	91-104
1:160	Average %	101
	Range %	94-108

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.846

2.694

2.770

2.609

2.337

2.367

2.352

2.191

2.5

1.679

1.748

1.714

1.553

1.25

1.247

1.229

1.238

1.077

0.625

0.737

0.721

0.729

0.568

0.312

0.512

0.533

0.523

0.362

0.156

0.303

0.284

0.294

0.133

0.164

0.158

0.161

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The human S100A12 ELISA Kit quantitates human S100A12 levels in serum and plasma. S100A12, also called calgranulin C, is a calcium-binding protein highly expressed in neutrophils and lowly expressed in lymphocytes and monocytes. It is secreted and upregulated during acute and chronic infection. Upon release, S100A12 functions as a proinflammatory alarmin. Elevated plasma levels of S100A12 are a new biomarker for many inflammatory diseases due to their significantly increased expression at inflamed sites. Additionally, S100A12 has been detected in many types of cancer such as oropharyngeal squamous cell carcinoma (OPSCC) and colorectal cancer, and is associated with proliferation, invasion, and migration of tumor cells. S100A12 expression is related to pathological features, including the tumor grade and stage as well as lymph node metastasis status.

This kit employs the sandwich-ELISA mechanism in conjugation with S100A12 antibody-S100A12 antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of S100A12 in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, high recovery, and a precision of less than 10%.

Single-Cell RNA Sequencing of PBMCs Identified Junction Plakoglobin (JUP) as Stratification Biomarker for Endometriosis T Andrieu, A Duo, L Duempelmann, M Patzak,International Journal of Molecular Sciences,2024
Identification of Neutrophil Extracellular Trap-Related Gene Expression Signatures in Ischemia Reperfusion Injury During Lung Transplantation: A Transcriptome Analysis and Clinical Validation W Wu,Journal of inflammation research,2024
The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis X Bai,BMC immunology,2022
Discovery, validation, and diagnostic ability of multiple protein-based biomarkers in saliva and gingival crevicular fluid to distinguish between health and periodontal diseases MM Grant,Journal of clinical periodontology,2022
The pathogenic roles of S100A8/A9 and S100A12 in antineutrophil cytoplasmic antibody-associated vasculitis PC Xu,ResearchSquare,2022
Clinical features and nasal inflammation in asthma and allergic rhinitis M Chen,Clinical and Experimental Immunology,2022
High Serum S100A12 Levels Predict Poor Outcome After Acute Primary Intracerebral Hemorrhage SZ Qiu,Neuropsychiatric disease and treatment,2021
Assessment of EN-RAGE, sRAGE and EN-RAGE/sRAGE as potential biomarkers in patients with autoimmune hepatitis R Wu,Journal of Translational Medicine,2020
Serum S100A12 levels are correlated with clinical severity in patients with dermatomyositis-associated interstitial lung disease Lou Y,Journal of International Medical Research,2019
Relation of postoperative serum S100A12 levels to delirium and cognitive dysfunction occurring after hip fracture surgery in elderly patients Li QH, et al,Brain and Behavior,2018
Myeloid related proteins are up-regulated in autoimmune thyroid diseases and activate toll-like receptor 4 and pro-inflammatory cytokines in vitro Peng S.et al,Int Immunopharmacol,2018
Serum S100A12 as a prognostic biomarker of severe traumatic brain injury Ma-JingFeng.et al,Clinica Chimica Acta,2018
Diagnostic value of amniotic fluid inflammatory biomarkers for subclinical chorioamnionitis. Liu Y.et al,Int J Gynaecol Obstet.,2016
Serum S100A12 and progression of coronary artery calcification over 4 years in hemodialysis patients Wang Y. et al,American Journal of Nephrology,2015
Diagnostic importance of S100A9 and S100A12 in breast cancer M Gunaldi. et al,Biomedicine & Pharmacotherapy,2015
Polyunsaturated fatty acidsAugment tumoricidal action of 5-fluorouracil on gastric cancer cells by their action on vascular endothelial growth factor, tumor necrosis factor-a and lipid metabolism related factors Haining Yu. et al,ARCHIVES OF MEDICAL SCIENCE,2015
Increased Plasma S100A12 Levels Are Associated With Diabetic Retinopathy and Prognostic Biomarkers of Macrovascular Events in Type 2 Diabetic Patients Dong N. et al,Invest Ophthalmol Vis Sci.,2015
Serum S100A12 Levels Are Correlated With the Presence and Severity of Coronary Artery Disease in Patients With Type 2 Diabetes Mellitus Zhao P et al,J Investig Med,2013
Serum S100A12 levels are associated with the presence and severity of obstructive sleep apnea syndrome in male patients Shi YK et al,Sleep Breath,2013
Serum S100A12 concentrations are correlated with angiographic coronary lesion complexity in patients with coronary artery disease Liu J et al,Scand J Clin Lab Invest.,2013
S100A12 levels in synovial fluidMay reflect clinical severity in patients with primary knee osteoarthritis Li-chun Wang et al,Biomarkers.,2013

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.

Gene References into Functions

S100A12 activates NLPR3 inflammasomes to induce MUC5AC production in airway epithelial cells. ATP induces MUC5AC production in a mechanistically similar mode to S100A12. PMID: 29906464
The results suggest that S100A12 does not participate in the induction of inflammation in dental pulp. However, RAGE can participate in the inflammation in the pulp of males. PMID: 28834384
S100A12 was a significant predictor of lung alveolar infiltration (OR 2.60, 95%CI 1.35-5.00, p = 0.004). These results suggest that S100A12 has the potential to assess the extent of alveolar infiltration in Pulmonary tuberculosis. PMID: 27539060
Results indicated that S100A12 could increase the expression of MMP-2, MMP-9, and vascular cell adhesion molecule 1 (VCAM-1) in HASMCs via activation of ERK1/2 signal pathway, which leads to injury of HASMCs. PMID: 28816402
S100A12 binds to CD36 in the low nanomolar range at the CD36 thrombospondin-1 binding site. PMID: 27734162
data on the antimicrobial activity of S100A12 have been reported. Proinflammatory role of S100A12 is supported by another newly found receptor, Toll-like receptor 4 (TLR4). PMID: 28110121
Report role of fecal S100A12 assay in the diagnosis and management of inflammatory bowel disease. PMID: 28735301
The aim of this mini-review was to outline the pleiotropic actions of S100A12 and to highlight the potential clinical importance of this protein in kidney and cardiovascular diseases. [review] PMID: 29080693
serum levels had significant, positive correlations with intensive care unit length of stay, 28-day mortality, and in-hospital mortality after major abdominal surgery PMID: 27689623
The binding interface between S100A12 and the V domain of RAGE has been identified and mapped. PMID: 27598566
S100A12 functions as a proinflammatory cytokine and activates dermal fibroblasts, causing dermal fibrosis PMID: 27840235
These data suggest that S100A12 is part of an innate and adaptive inducible antimicrobial network that contributes to host defense against mycobacteria in infected macrophages PMID: 27355424
Among the investigated S100-proteins, S100A12 showed the closest association with disease activity and therapeutic response and might therefore provide a valuable biomarker for psoriasis PMID: 26333514
the expression of S100A12 protein and mRNA was downregulated in a large number of clinical samples of GC. Low expression of S100A12 exhibited a marked propensity toward the clinicopathologic features such as tumor size, depth of invasion, TNM stage, Lauren classification, tumor cell differentiation, and poor survival in GC patients. PMID: 26638166
Serum S100A12 was significantly higher in rheumatoid arthritis patients than controls, and was correlated with disease activity. PMID: 26767827
an elevated serum level of S100A12 was an independent determinant of the progression of abdominal aortic calcification determined by lateral lumbar X-ray in Hemodialysis patients. PMID: 26914918
Our findings indicate that chitotriosidase and S10012A are useful in diagnosis and detection of subclinical inflammation and/or assessment of disease activity in FMF patients. PMID: 26339162
This is the first kinetics study of levels of RAGE main isoforms and ligands during ARDS. Elevated sRAGE, HMGB1 and S100A12, with decreased esRAGE and AGEs, were found to distinguish patients with ARDS from those without. PMID: 26274928
serum level elevated in acute liver failure PMID: 25825217
S100A12 is a biomarker of chronic heart failure that may also predict major cardiovascular events in patients with chronic heart failure. PMID: 25438075
High mRNA expression of S100A12 is associated with bladder transitional cell carcinoma. PMID: 25854354
These findings suggested that S100A12 is an effective marker for inflammatory diseases. PMID: 25650963
High serum S100A12 expression is associated with poor response to therapy in Crohn's disease. PMID: 25625487
Suggest that an elevated S100A12 level could play a crucial role in systemic inflammation and may be a promising biomarker for predicting perioperative complications in patients with thoracic aortic dissection. PMID: 24691129
These assays showed that S100A12 is induced in response to Helicobacter pylori infection and inhibits bacterial growth and viability in vitro by binding nutrient zinc. PMID: 25964473
S100A12 might participate in the damage of biliary epithelial cells and hepatocytes in primary biliary cirrhosis. PMID: 25313445
Using a computational approach, the study investigated the modulation of protein structure by different ions in the solution, at different ionic strengths. PMID: 24944024
Data indicate that resistin, S100A12 and soluble receptor for advanced glycation end products (sRAGE) are involved in the pathophysiology of Kawasaki disease (KD). PMID: 23171632
The S100A12 protein was significantly associated with synovitis score in rheumatoid arthritis patients. PMID: 25282581
highlight EN-RAGE as an inflammatory marker for future coronary heart disease (CHD) in a general population, beyond traditional CHD risk factors and inflammatory markers PMID: 25341801
S100A12 is associated with duration of cardiopulmonary bypass, pulmonary inflammation, hypoxia and prolonged mechanical ventilation and may contribute to acute lung injury in cardiac surgery patients. PMID: 24887223
The antimicrobial peptide calcitermin was isolated from human airway secretions and targets Gram-negative bacteria. PMID: 11522286
Serum S100A12 concentrations are correlated with angiographic coronary lesion complexity in patients with coronary artery disease. PMID: 24341566
Correlation of human S100A12 and high-sensitivity C-reactive protein as gingival crevicular fluid and serum markers of inflammation in chronic periodontitis and type 2 diabetes. PMID: 24378957
Excessive expression of the S100A12 gene in uremic leukocytes is relevant to its increased serum concentration, particularly in those affected with cardiovascular disease. PMID: 23921255
S100A12 and hBD2 correlate with the fecal microbiota thus linking the intestinal innate immune response to the bacterial colonization. PMID: 24307989
EN-RAGE inflammatory ligand has an increased expression in Takayasu's arteritis patients. PMID: 23398829
High S100A12 levels are associated with the presence and severity of coronary artery disease in patients with type 2 diabetes mellitus. PMID: 23609464
High S100A12 expression is associated with intestinal inflammation and relapse in inflammatory bowel disease. PMID: 23377171
The results indicate that plasma S100A12 level is an independent predictor for two-year all-cause mortality. A simple integer scoring system was therefore established for predicting mortality on the basis of plasma S100A12 levels. PMID: 23324110
S100A12 could be a novel biomarker for predicting cardiovascular events for predicting MACE in patients with stable CAD. PMID: 22786469
Fecal S100A12 levels were significantly higher in patients with severe necrotizing enterocolitis (NEC) at onset of disease and also, in contrast to fecal calprotectin, at 4-10 days before onset of NEC compared with unaffected reference infants. PMID: 22796048
Significant relationship was found between MMP9 and HMGB1 and EN-RAGE in diabetic patients. PMID: 22549347
Data indicate that S100A12 is up-regulated in Thoracic Aortic Aneurysm Dissection (TAAD) and may contribute to the pathogenesis of TAAD by initiating apoptosis of SMC, at least in part via increased oxidative stress. PMID: 22818064
these data indicate the possible involvement of S100A12 in the development of osteoarthritis by up-regulating MMP-13 and VEGF via p38 MAPK and NF-kappaB pathways. PMID: 22609404
Although S100A12 levels are not elevated in patients with decreased kidney function, a relation to markers of inflammatory disease is found. PMID: 21822023
Levels of serum RAGE are reduced in patients with juvenile rheumatoid arthritis and correlate negatively with disease activity and S100A12 levels. PMID: 21724696
Suggest that elevated plasma EN-RAGE and decreased sRAGE level could play a crucial role in systemic inflammation and carotid atherosclerosis in peritoneal dialysis patients. PMID: 21906738
Transgenic expression of S100A12 in the lung of mice does not exacerbate lung inflammation in a model of OVA-induced allergic inflammation. PMID: 21418345
plasma S100A12 protein level is strongly associated with the prevalence of cardiovascular disease in hemodialysis patients. PMID: 21258041

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Subcellular Location

Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. Note=Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation is secreted via a microtubule-mediated, alternative pathway.

Protein Families

S-100 family

Tissue Specificity

Predominantly expressed by neutrophils, monocytes and activated macrophages. Expressed by eosinophils and macrophages in asthmatic airways in regions where mast cells accumulate. Found in high concentrations in the serum of patients suffering from various

Database Links

HGNC: 10489

OMIM: 603112

KEGG: hsa:6283

STRING: 9606.ENSP00000357726

UniGene: Hs.19413

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