Human S100 calcium binding protein A12/Calgranulin-C(S100A12) ELISA Kit

Code CSB-E13095h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name S100 calcium binding protein A12
Alternative Names CAAF1 ELISA Kit; CAGC ELISA Kit; Calcitermin ELISA Kit; Calcium-binding protein in amniotic fluid 1 ELISA Kit; Calgranulin C ELISA Kit; Calgranulin-C ELISA Kit; Calgranulin-related protein ELISA Kit; CGRP ELISA Kit; EN RAGE ELISA Kit; EN-RAGE ELISA Kit; ENRAGE ELISA Kit; Extracellular newly identified RAGE-binding protein ELISA Kit; migration inhibitory factor-related protein 6 ELISA Kit; MRP6 ELISA Kit; Neutrophil S100 protein ELISA Kit; p6 ELISA Kit; Protein S100 A12 ELISA Kit; S100 calcium binding protein A12 ELISA Kit; S100 calcium-binding protein A12 (calgranulin C) ELISA Kit; S100 calcium-binding protein A12 ELISA Kit; S100A12 ELISA Kit; S10AC_HUMAN ELISA Kit
Abbreviation S100A12
Uniprot No. P80511
Species Homo sapiens (Human)
Sample Types
Detection Range
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Typical Data  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


Target Data

Function S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.
Gene References into Functions
  1. S100A12 activates NLPR3 inflammasomes to induce MUC5AC production in airway epithelial cells. ATP induces MUC5AC production in a mechanistically similar mode to S100A12. PMID: 29906464
  2. The results suggest that S100A12 does not participate in the induction of inflammation in dental pulp. However, RAGE can participate in the inflammation in the pulp of males. PMID: 28834384
  3. S100A12 was a significant predictor of lung alveolar infiltration (OR 2.60, 95%CI 1.35-5.00, p = 0.004). These results suggest that S100A12 has the potential to assess the extent of alveolar infiltration in Pulmonary tuberculosis. PMID: 27539060
  4. Results indicated that S100A12 could increase the expression of MMP-2, MMP-9, and vascular cell adhesion molecule 1 (VCAM-1) in HASMCs via activation of ERK1/2 signal pathway, which leads to injury of HASMCs. PMID: 28816402
  5. S100A12 binds to CD36 in the low nanomolar range at the CD36 thrombospondin-1 binding site. PMID: 27734162
  6. data on the antimicrobial activity of S100A12 have been reported. Proinflammatory role of S100A12 is supported by another newly found receptor, Toll-like receptor 4 (TLR4). PMID: 28110121
  7. Report role of fecal S100A12 assay in the diagnosis and management of inflammatory bowel disease. PMID: 28735301
  8. The aim of this mini-review was to outline the pleiotropic actions of S100A12 and to highlight the potential clinical importance of this protein in kidney and cardiovascular diseases. [review] PMID: 29080693
  9. serum levels had significant, positive correlations with intensive care unit length of stay, 28-day mortality, and in-hospital mortality after major abdominal surgery PMID: 27689623
  10. The binding interface between S100A12 and the V domain of RAGE has been identified and mapped. PMID: 27598566
  11. S100A12 functions as a proinflammatory cytokine and activates dermal fibroblasts, causing dermal fibrosis PMID: 27840235
  12. These data suggest that S100A12 is part of an innate and adaptive inducible antimicrobial network that contributes to host defense against mycobacteria in infected macrophages PMID: 27355424
  13. Among the investigated S100-proteins, S100A12 showed the closest association with disease activity and therapeutic response and might therefore provide a valuable biomarker for psoriasis PMID: 26333514
  14. the expression of S100A12 protein and mRNA was downregulated in a large number of clinical samples of GC. Low expression of S100A12 exhibited a marked propensity toward the clinicopathologic features such as tumor size, depth of invasion, TNM stage, Lauren classification, tumor cell differentiation, and poor survival in GC patients. PMID: 26638166
  15. Serum S100A12 was significantly higher in rheumatoid arthritis patients than controls, and was correlated with disease activity. PMID: 26767827
  16. an elevated serum level of S100A12 was an independent determinant of the progression of abdominal aortic calcification determined by lateral lumbar X-ray in Hemodialysis patients. PMID: 26914918
  17. Our findings indicate that chitotriosidase and S10012A are useful in diagnosis and detection of subclinical inflammation and/or assessment of disease activity in FMF patients. PMID: 26339162
  18. This is the first kinetics study of levels of RAGE main isoforms and ligands during ARDS. Elevated sRAGE, HMGB1 and S100A12, with decreased esRAGE and AGEs, were found to distinguish patients with ARDS from those without. PMID: 26274928
  19. serum level elevated in acute liver failure PMID: 25825217
  20. S100A12 is a biomarker of chronic heart failure that may also predict major cardiovascular events in patients with chronic heart failure. PMID: 25438075
  21. High mRNA expression of S100A12 is associated with bladder transitional cell carcinoma. PMID: 25854354
  22. These findings suggested that S100A12 is an effective marker for inflammatory diseases. PMID: 25650963
  23. High serum S100A12 expression is associated with poor response to therapy in Crohn's disease. PMID: 25625487
  24. Suggest that an elevated S100A12 level could play a crucial role in systemic inflammation and may be a promising biomarker for predicting perioperative complications in patients with thoracic aortic dissection. PMID: 24691129
  25. These assays showed that S100A12 is induced in response to Helicobacter pylori infection and inhibits bacterial growth and viability in vitro by binding nutrient zinc. PMID: 25964473
  26. S100A12 might participate in the damage of biliary epithelial cells and hepatocytes in primary biliary cirrhosis. PMID: 25313445
  27. Using a computational approach, the study investigated the modulation of protein structure by different ions in the solution, at different ionic strengths. PMID: 24944024
  28. Data indicate that resistin, S100A12 and soluble receptor for advanced glycation end products (sRAGE) are involved in the pathophysiology of Kawasaki disease (KD). PMID: 23171632
  29. The S100A12 protein was significantly associated with synovitis score in rheumatoid arthritis patients. PMID: 25282581
  30. highlight EN-RAGE as an inflammatory marker for future coronary heart disease (CHD) in a general population, beyond traditional CHD risk factors and inflammatory markers PMID: 25341801
  31. S100A12 is associated with duration of cardiopulmonary bypass, pulmonary inflammation, hypoxia and prolonged mechanical ventilation and may contribute to acute lung injury in cardiac surgery patients. PMID: 24887223
  32. The antimicrobial peptide calcitermin was isolated from human airway secretions and targets Gram-negative bacteria. PMID: 11522286
  33. Serum S100A12 concentrations are correlated with angiographic coronary lesion complexity in patients with coronary artery disease. PMID: 24341566
  34. Correlation of human S100A12 and high-sensitivity C-reactive protein as gingival crevicular fluid and serum markers of inflammation in chronic periodontitis and type 2 diabetes. PMID: 24378957
  35. Excessive expression of the S100A12 gene in uremic leukocytes is relevant to its increased serum concentration, particularly in those affected with cardiovascular disease. PMID: 23921255
  36. S100A12 and hBD2 correlate with the fecal microbiota thus linking the intestinal innate immune response to the bacterial colonization. PMID: 24307989
  37. EN-RAGE inflammatory ligand has an increased expression in Takayasu's arteritis patients. PMID: 23398829
  38. High S100A12 levels are associated with the presence and severity of coronary artery disease in patients with type 2 diabetes mellitus. PMID: 23609464
  39. High S100A12 expression is associated with intestinal inflammation and relapse in inflammatory bowel disease. PMID: 23377171
  40. The results indicate that plasma S100A12 level is an independent predictor for two-year all-cause mortality. A simple integer scoring system was therefore established for predicting mortality on the basis of plasma S100A12 levels. PMID: 23324110
  41. S100A12 could be a novel biomarker for predicting cardiovascular events for predicting MACE in patients with stable CAD. PMID: 22786469
  42. Fecal S100A12 levels were significantly higher in patients with severe necrotizing enterocolitis (NEC) at onset of disease and also, in contrast to fecal calprotectin, at 4-10 days before onset of NEC compared with unaffected reference infants. PMID: 22796048
  43. Significant relationship was found between MMP9 and HMGB1 and EN-RAGE in diabetic patients. PMID: 22549347
  44. Data indicate that S100A12 is up-regulated in Thoracic Aortic Aneurysm Dissection (TAAD) and may contribute to the pathogenesis of TAAD by initiating apoptosis of SMC, at least in part via increased oxidative stress. PMID: 22818064
  45. these data indicate the possible involvement of S100A12 in the development of osteoarthritis by up-regulating MMP-13 and VEGF via p38 MAPK and NF-kappaB pathways. PMID: 22609404
  46. Although S100A12 levels are not elevated in patients with decreased kidney function, a relation to markers of inflammatory disease is found. PMID: 21822023
  47. Levels of serum RAGE are reduced in patients with juvenile rheumatoid arthritis and correlate negatively with disease activity and S100A12 levels. PMID: 21724696
  48. Suggest that elevated plasma EN-RAGE and decreased sRAGE level could play a crucial role in systemic inflammation and carotid atherosclerosis in peritoneal dialysis patients. PMID: 21906738
  49. Transgenic expression of S100A12 in the lung of mice does not exacerbate lung inflammation in a model of OVA-induced allergic inflammation. PMID: 21418345
  50. plasma S100A12 protein level is strongly associated with the prevalence of cardiovascular disease in hemodialysis patients. PMID: 21258041
  51. Circulating S100A12 and soluble RAGE are both elevated in hemodialysis patients. However, only S100A12 associates with mortality, partly explained by its links with inflammation. PMID: 20847094
  52. Vascular smooth muscle S100A12 accelerates atherosclerosis and augments atherosclerosis-triggered osteogenesis, reminiscent of features associated with plaque instability. PMID: 20966394
  53. treatment of THP-1 cells with 100 microg of CRP/ml/10(6) cells for 24 h, augmented the expression of RAGE and EN-RAGE genes PMID: 19201044
  54. Data suggest that the S100A12 expression on circulating endothelial cells may be involved in the development of coronary artery lesions in children with Kawasaki disease. PMID: 20461025
  55. carboxylated N-glycans on RAGE enhance binding potential and promote receptor clustering and subsequent signaling events following oligomeric S100A12 binding. PMID: 20512925
  56. study demonstrates that besides RAGE also scavenger receptors contribute to distribution, tissue association and elimination of circulating proinflammatory S100A12 PMID: 20025991
  57. Data suggest that increased S100A12 levels may point to synovial inflammation of the temporomandibular joint in juvenile idiopathic arthritis. PMID: 20437698
  58. Report increased expression of RAGE and EN-RAGE in non-diabetic pre-mature coronary artery disease. PMID: 19576587
  59. S100A12 expression is sufficient to activate pathogenic pathways through the modulation of oxidative stress, inflammation and vascular remodeling in vivo. PMID: 19875725
  60. Circulating CGRP in fulminant hepatic failure seems to reflect hemodynamic changes in the systemic rather than in the cerebral circulation. PMID: 11884208
  61. Deficiency of CGRP in Familial Dysautonomia patients may explain some of their symptoms. PMID: 11884210
  62. Proinflammatory myeloid-related protein S100A12 induces a dose- and time-dependent activation of the HIV-1 long terminal repeat promoter region that can be blocked by specific polyclonal antibody and by physical denaturation of the protein. PMID: 12218151
  63. Fecal S100A12 is expressed in feces of children with Crohn's disease PMID: 16804393
  64. the function of S100A12 is suppressed by olopatadine in THP-1 monocytes PMID: 16864903
  65. Ca2+ binding creates two symmetric hydrophobic surfaces on Ca2+-calgranulin C that allow calgranulin C to bind to the C-type immunoglobulin domain of the receptor for advanced glycation products (RAGE). PMID: 17158877
  66. Serum calprotectin and S100A12 are increased in children with inflammatory bowel disease and indicate disease activity. PMID: 17852869
  67. Calculating the S100A12:sRAGE ratio might help to detect patients with KD who are at risk of being unresponsive to IVIG therapy. PMID: 18050248
  68. These results suggest that S100A12 may be a sensitive marker of subclinical inflammation and that an increased S100A12 level may be related to the high peritoneal solute transport rate PMID: 18257809
  69. Mast cell and monocyte recruitment by S100A12 PMID: 18292089
  70. thermodynamic study of the recombinant S100A12 using circular dichroism, fluorescence and isothermal titration calorimetry PMID: 18346834
  71. Human S100A12 is markedly overexpressed in inflammatory compartments, and elevated serum levels of S100A12 are found in patients suffering from various inflammatory, neurodegenerative, metabolic, and neoplastic disorders. PMID: 18443896
  72. S100A12 protein is involved in the acceleration of atherosclerosis in hemodialysis patients PMID: 18663285
  73. Oligomerization and target recognition by S100A12 is regulated by zinc and calcium. A potential role of calcium-binding S100 proteins in zinc metabolism and, in particular, the role of S100A12 in the cross talk between zinc and calcium in cell signaling. PMID: 19386136
  74. The zinc complex structure shows significant differences from those of both calcium-loaded and apo-S100A12 structures, and comparisons suggest an explanation for the zinc-induced 1500-fold increase in calcium affinity. PMID: 19501594
  75. increased expression in sera, synovial tissues and in PBMC of rheumatoid arthritis patients PMID: 19530996
  76. S100A12 may represent a new marker of atheroma and may protect advanced atherosclerotic lesions from rupture by inhibiting excessive matrix metalloproteinase (MMP)-2 and MMP-9 colocalized in dimeric zinc-dependent S100A12 complexes. PMID: 19542470
  77. S100A12 induces inflammatory activation of endothelial cells [review]. PMID: 12697438
  78. S100A12 is highly expressed and shows a close correlation with disease activity in a systemic human vasculitis (Kawasaki disease). PMID: 12699958
  79. S100A12 is highly expressed in inflammatory bowel disease and down-regulated by anti-TNF therapy. Secretion of S100A12 is induced by TNF. PMID: 12740341
  80. Serum concentrations of S100A12 are useful markers for monitoring disease activity in juvenile rheumatoid arthritis. PMID: 15077313

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Subcellular Location Secreted, Cytoplasm, Cytoplasm, cytoskeleton, Cell membrane, Peripheral membrane protein
Protein Families S-100 family
Tissue Specificity Predominantly expressed by neutrophils, monocytes and activated macrophages. Expressed by eosinophils and macrophages in asthmatic airways in regions where mast cells accumulate. Found in high concentrations in the serum of patients suffering from various
Database Links

HGNC: 10489

OMIM: 603112

KEGG: hsa:6283

STRING: 9606.ENSP00000357726

UniGene: Hs.19413

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