Human Secretoglobin family 1D member 2(SCGB1D2) ELISA kit

Code CSB-EL020814HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
secretoglobin, family 1D, member 2
Alternative Names
SCGB1D2 ELISA kit; LIPHB ELISA kit; LPNBSecretoglobin family 1D member 2 ELISA kit; Lipophilin-B ELISA kit
Abbreviation
SCGB1D2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 ng/mL-2000 ng/mL
Sensitivity
7.81 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SCGB1D2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %89
Range %84-93
1:10Average %98
Range %84-102
1:20Average %94
Range %90-98
1:40Average %103
Range %99-110
Recovery
The recovery of human SCGB1D2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8680-90
EDTA plasma (n=4)9288-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
20002.436 2.351 2.394 2.233
10001.872 1.807 1.840 1.679
5001.154 1.182 1.168 1.007
2500.795 0.834 0.815 0.654
1250.428 0.448 0.438 0.277
62.50.362 0.341 0.352 0.191
31.250.231 0.217 0.224 0.063
00.163 0.159 0.161
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human SCGB1D2 ELISA Kit was designed for the quantitative measurement of Human SCGB1D2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.81 ng/mL.

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Target Background

Function
(From Uniprot)
May bind androgens and other steroids, may also bind estramustine, a chemotherapeutic agent used for prostate cancer. May be under transcriptional regulation of steroid hormones.
Gene References into Functions
  1. The data indicate that MGB1, MGB2 and LIPB mRNAs are expressed at low levels in human tissues but basal expression is upregulated in ovarian cancer. PMID: 24603286
  2. lipophilin B gene overexpression correlates with a less aggressive tumor phenotype and represents a novel ovarian carcinoma prognostic factor PMID: 23819652
  3. Serum antibodies to this protein are detected in late stage breast cancer patients. PMID: 12576445
  4. The present report demonstrates, for the first time, lipophilin B expression in the pituitary and its apparent down-regulation in pituitary adenomas. PMID: 15668787
  5. We conclude that expression of LPB is preferentially but not exclusively restricted to breast tissue. PMID: 17163411
  6. Ectopic overexpression of mammaglobin and lipophilin B did not affect the cell proliferation rate of Hs578T breast carcinoma cells in vitro. PMID: 18630503

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Subcellular Location
Secreted.
Protein Families
Secretoglobin family, Lipophilin subfamily
Tissue Specificity
Highest expression was found in skeletal muscle. Expressed as well in thymus, trachea, kidney, steroid responsive tissues (prostate, testis, uterus, breast and ovary) and salivary gland.
Database Links

HGNC: 18396

OMIM: 615061

KEGG: hsa:10647

STRING: 9606.ENSP00000244926

UniGene: Hs.204096

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