Human Thrombospondin-4(THBS4) ELISA kit

Code CSB-EL023490HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P35443
Lead Time 5-7 working days
Abbreviation THBS4
Protein Biological Process 1 Cell Adhesion
Target Name thrombospondin 4
Alias TSP4,
Species Homo sapiens (Human)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma
Detection Range 7.8 ng/mL-500 ng/mL
Sensitivity 1.95 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to the thrombospondin protein family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentamer and can bind to heparin and calcium. Studies of the rat counterpart suggest that this protein may be involved in local signaling in the developing and adult nervous system.
HGNC 11788
RGD 62046
MGI 1101779
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human THBS4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %89-97
1:2Average %101
Range %98-105
1:4Average %99
Range %95-104
1:8Average %92
Range %88-96
Recovery
The recovery of human THBS4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-99
EDTA plasma (n=4)9389-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
5002.673 2.698 2.686 2.539
2502.034 2.086 2.060 1.913
1251.242 1.268 1.255 1.108
62.50.695 0.675 0.685 0.538
31.20.403 0.415 0.409 0.262
15.60.278 0.299 0.289 0.142
7.80.217 0.227 0.222 0.075
00.146 0.148 0.147  
References
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Function Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner (By similarity).
Subcellular Location Endoplasmic reticulum, Sarcoplasmic reticulum, Secreted, Secreted, extracellular space, Secreted, extracellular space, extracellular matrix
Protein Families Thrombospondin family
Database Links

HGNC: 11788

OMIM: 600715

KEGG: hsa:7060

STRING: 9606.ENSP00000339730

UniGene: Hs.211426

Pathway PI3K-Akt signaling pathway
Focal adhesion

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