Human V-type proton ATPase 116 kDa subunit a isoform 2(ATP6V0A2) ELISA kit

Instructions
Code CSB-EL002386HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name ATPase, H+ transporting, lysosomal V0 subunit a2
Alternative Names a2 ELISA Kit; A2V ATPase ELISA Kit; ARCL ELISA Kit; ATP6a2 ELISA Kit; ATP6N1D ELISA Kit; ATP6V0A2 ELISA Kit; ATPase, H+ transporting, lysosomal V0 subunit a isoform 2 ELISA Kit; ATPase, H+ transporting, lysosomal V0 subunit a2 ELISA Kit; Infantile malignant osteopetrosis ELISA Kit; J6B7 ELISA Kit; Lysosomal H(+) transporting ATPase V0 subunit a2 ELISA Kit; Lysosomal H(+)-transporting ATPase V0 subunit a2 ELISA Kit; regeneration and tolerance factor ELISA Kit; Stv1 ELISA Kit; TJ6 ELISA Kit; TJ6M ELISA Kit; TJ6s ELISA Kit; V ATPase 116 kDa isoform a2 ELISA Kit; V type proton ATPase 116 kDa subunit a ELISA Kit; V type proton ATPase 116 kDa subunit a isoform 2 ELISA Kit; V-ATPase 116 kDa isoform a2 ELISA Kit; V-type proton ATPase 116 kDa subunit a isoform 2 ELISA Kit; Vacuolar proton translocating ATPase 116 kDa subunit a ELISA Kit; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2 ELISA Kit; Vph1 ELISA Kit; VPP2_HUMAN ELISA Kit; WSS ELISA Kit
Abbreviation ATP6V0A2
Uniprot No. Q9Y487
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ATP6V0A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-95  
1:2 Average % 102  
Range % 97-107  
1:4 Average % 91  
Range % 85-97  
1:8 Average % 97  
Range % 91-103  
Recovery
The recovery of human ATP6V0A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 97 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.281 2.298 2.290 2.098  
1000 1.615 1.642 1.629 1.437  
500 0.995 0.954 0.975 0.783  
250 0.634 0.626 0.630 0.438  
125 0.468 0.449 0.459 0.267  
62.5 0.358 0.337 0.348 0.156  
31.25 0.287 0.298 0.293 0.101  
0 0.191 0.193 0.192    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Part of the proton channel of V-ATPases. Essential component of the endosomal pH-sensing machinery. May play a role in maintaining the Golgi functions, such as glycosylation maturation, by controlling the Golgi pH. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation
Gene References into Functions
  1. Data suggest that missense mutations in ATP6V0A2 and ATP6V0A4 that cause either cutis laxa or distal renal tubular acidosis result in enzyme subunits that are unstable, retained in endoplasmic reticulum (rather than transported to Golgi and cell membrane), and quickly degraded by proteasomes despite full glycosylation. PMID: 29311258
  2. Study shows how tumor associated a2-isoform V-ATPase can induce neutrophil migration by stimulating autocrine secretion of IL-8, suggesting a mechanism for the creation of a level of inflammation that favors cancer growth. PMID: 27845385
  3. In cisplatin resistant cells, shRNA mediated inhibition of V-ATPase-V0a2 enhanced sensitivity towards both cisplatin and carboplatin. PMID: 26899534
  4. a2V deficiency disrupts the endolysosomal route in Notch and TGF signaling, thereby impairing mammary gland development. PMID: 27809299
  5. Senescence-associated impaired expression of ATP6V0A2 triggers changes in Golgi structure and glycosylation in old fibroblasts, which demonstrates a role of ATP6V0A2 in cellular senescence program. PMID: 26611489
  6. the results from this study demonstrate that the a2-subunit isoform of Vacuolar ATPase regulates Notch signaling in breast tumor cells PMID: 26418877
  7. The granule-associated a2V isoform has a role in maintaining a pH gradient within the cell between the cytosol and granules in neutrophils. PMID: 25877929
  8. Case Report: novel ATP6V0A2 mutations in an infant with cutis laxa. PMID: 24815019
  9. Expression of a2 vacuolar ATPase in spermatozoa is associated with semen quality and chemokine-cytokine profiles in infertile men. PMID: 23936208
  10. Mutations in the ATP6V0A2 gene is associated with autosomal recessive cutis laxa. PMID: 22773132
  11. A mechanism is described by which tumor-associated macrophages mature via a nontraditional cytokine-like signal, the a2NTD peptide. PMID: 21178005
  12. Data show that the V-ATPase a2-subunit might actually be embedded into and/or closely associated with membrane phospholipids even in the absence of any obvious predicted transmembrane segments. PMID: 20669186
  13. Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO. PMID: 20153292
  14. Studies indicate that mutations in the ATP6V0A2 gene were found in families with autosomal recessive cutis laxa. PMID: 19401719
  15. RTF (Regeneration and tolerance factor), the alpha-2 isoform of the alpha subunit of vacuolar ATPase, has a role in controlling IL-1 beta secretion by regulating P2X7 activity. PMID: 15301855
  16. cells were not susceptible to apoptosis when the 70-kDa RTF was present but were when the 50-kDa RTF was present; the increase in levels of the 50-kDa RTF on cells from HIV-positive individuals is important in preventing apoptosis PMID: 15358640
  17. RTF is constitutively expressed at endometrial and decidual level, and its up-regulation during the secretory phase of the cycle may be relevant in mediating some immune-related aspects of uterine physiology. PMID: 15373763
  18. Its role in organellar proton pumping suggests that hTJ6 function may participate in protein trafficking/processing. PMID: 16113235
  19. Data suggest a role for the N-terminus domain of the a2 isoform of vacuolar ATPase in the regulation of IL-1beta pro-inflammatory cytokine production at the fetal-maternal interface. PMID: 17295899
  20. Study identified loss-of-function mutations in ATP6V0A2, encoding the a2 subunit of the V-type H+ ATPase, in several families with autosomal recessive cutis laxa type II or wrinkly skin syndrome. PMID: 18157129
  21. the relationship between ATP6V0A2 mutations, the glycosylation defect and the autosomal recessive cutis laxa type II phenotype is discussed [review] PMID: 19171192
  22. Loss-of-function mutations in ATP6V0A2 lead to tropoelastin aggregation in the Golgi and increased apoptosis of elastogenic cells. PMID: 19321599

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Involvement in disease Cutis laxa, autosomal recessive, 2A (ARCL2A); Wrinkly skin syndrome (WSS)
Subcellular Location Cell membrane, Multi-pass membrane protein, Endosome membrane
Protein Families V-ATPase 116 kDa subunit family
Database Links

HGNC: 18481

OMIM: 219200

KEGG: hsa:23545

STRING: 9606.ENSP00000332247

UniGene: Hs.25786

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