Human V-type proton ATPase 116 kDa subunit a isoform 2(ATP6V0A2) ELISA kit

Code CSB-EL002386HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9Y487
Lead Time 5-7 working days
Protein Biological Process 2 Anion transport
Abbreviation ATP6V0A2
Protein Biological Process 1 Transport
Target Name ATPase, H+ transporting, lysosomal V0 subunit a2
Alias A2, ARCL, ATP6A2, ATP6N1D, J6B7, RTF, STV1, TJ6, TJ6M, TJ6S, VPH1, WSS, A2V-ATPase|ATPase, H+ transporting, lysosomal V0 subunit A2|regeneration and tolerance factor|v-ATPase 116 kDa|v-type proton A
Species Homo sapiens (Human)
Protein Biological Process 3 Hydrogen ion transport
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a subunit of the vacuolar ATPase (v-ATPase), an heteromultimeric enzyme that is present in intracellular vesicles and in the plasma membrane of specialized cells, and which is essential for the acidification of diverse cellular components. V-ATPase is comprised of a membrane peripheral V(1) domain for ATP hydrolysis, and an integral membrane V(0) domain for proton translocation. The subunit encoded by this gene is a component of the V(0) domain. Mutations in this gene are a cause of both cutis laxa type II and wrinkly skin syndrome.
HGNC 18481
RGD 621006
MGI 104855
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ATP6V0A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-95  
1:2 Average % 102  
Range % 97-107  
1:4 Average % 91  
Range % 85-97  
1:8 Average % 97  
Range % 91-103  
Recovery
The recovery of human ATP6V0A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 97 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.281 2.298 2.290 2.098  
1000 1.615 1.642 1.629 1.437  
500 0.995 0.954 0.975 0.783  
250 0.634 0.626 0.630 0.438  
125 0.468 0.449 0.459 0.267  
62.5 0.358 0.337 0.348 0.156  
31.25 0.287 0.298 0.293 0.101  
0 0.191 0.193 0.192    
References
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Function Part of the proton channel of V-ATPases. Essential component of the endosomal pH-sensing machinery. May play a role in maintaining the Golgi functions, such as glycosylation maturation, by controlling the Golgi pH. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation
Involvement in disease Cutis laxa, autosomal recessive, 2A (ARCL2A); Wrinkly skin syndrome (WSS)
Subcellular Location Cell membrane, Multi-pass membrane protein, Endosome membrane
Protein Families V-ATPase 116 kDa subunit family
Database Links

HGNC: 18481

OMIM: 219200

KEGG: hsa:23545

STRING: 9606.ENSP00000332247

UniGene: Hs.25786

Pathway Oxidative Phosphorylation
Synaptic vesicle cycle

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