Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human vaspin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
The recovery of human vaspin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Average % Recovery
EDTA plasma (n=4)
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Please confirm whether this kit can be used with Saliva samples and also suggest suitable protocol for saliva sample preparation.
Thanks for your inquiry. CSB-E09771h ,we used it to test human saliva before and the value was ND--62.5pg/ml. Here is the processing method on saliva sample preparation.You can take it as reference:Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles. Recommend to use fresh saliva samples.
Function (From Uniprot)
Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
Gene References into Functions
Elevated levels of vaspin were associated with 1.7 times increased cardiovascular disease risk. PMID: 29673927
vaspin rs2236242 polymorphism is not associated with metabolic syndrome in Egyptian women PMID: 27966294
vaspin decreased miR-33a levels, which in turn increased ABCA1 expression and cholesteorl efflux. PMID: 29653102
High vaspin expression is associated with osteoporosis and arterial stiffness. PMID: 28646476
Serum vaspin concentrations were significantly higher in the type-2 diabetes mellitus (T2DM) group than in the T2DM + macrovascular complications group. PMID: 29065866
Serum vaspin level may be a useful marker for the prediction of ovulation induction success in treatment with clomiphene citrate PMID: 28826039
vaspin can play a role as a new marker in the diagnosis, severity assessment, and prognosis of subarachnoid hemorrhage PMID: 25720423
Suggest vaspin could be involved in the pathophysiology of cirrhosis, but is not a good indicator of its origin or severity and does not impact the thrombotic activity of platelets. PMID: 28465646
Vaspin may play an important compensatory role in obesity and insulin resistance in elderly people. PMID: 28553648
Studied the effects of vaspin on lipopolysaccharide (LPS)induced acute respiratory distress syndrome (ARDS) in vivo and in vitro, and further explored the molecular basis and potential mechanisms underlying these effects. It was demonstrated that vaspin protects against LPSinduced ARDS through reversing EC barrier dysfunction via the suppression of inflammation, apoptosis and ROS generation in pulmonary ECs. PMID: 29039444
Serum vaspin cannot be used as an independent marker for the presence of coronary artery disease in the general population. PMID: 28482816
Vaspin is modified at all 3 predicted asparagine glycosylation sites near the reactive center loop. Biochemical data demonstrate that glycosylation does not hinder inhibition of the target protease kallikrein 7. Although binding affinity to heparin is slightly decreased, the protease inhibition reaction is still significantly accelerated in the presence of heparin. Glycosylation did not affect thermal stability. PMID: 28668641
Vaspin was associated with a reduced risk of insulin resistance independently of BMI and pubertal stage and the association was influenced by body fat and physical activity in these adolescents. PMID: 27513492
Study provides evidence that serum vaspin level could be regarded as the predictor for improvement of fatty liver and metabolic disturbance after laparoscopic vertical banded gastroplasty surgery in patients with severe obesity. PMID: 28746192
The minor A allele of vaspin rs2236242 polymorphism plays a protective role against obesity and diabetes among women in Upper Egypt. The serum vaspin concentration was lower in minor protective allele carriers. PMID: 28479386
In COPD, the meta-analysis of % predicted FEV1 in cases, the strongest association was shown for a splice variant in a previously unreported region, SERPINA12 (rs140198372, pmeta=5.72x10(-6)). PMID: 26917578
Together, these data show that vaspin binds heparin with high affinity (KD = 21 +/- 2 nm) and that binding takes place at a basic patch on top of beta-sheet A and is different from other heparin-binding serpins. PMID: 27941022
In patients with diabetic nephropathy (DNP), vaspin level does not change within the early stages of DNP; while it is higher in patients with decreased glomerular filtration rate, which may be related with increasing inflammation regardless of the stage of the kidney disease. PMID: 27216464
vaspin could be visibly observed within the detectable concentrations of vaspin up to 5nM in both buffer and serum conditions. The sensitivity of this LFSA developed in this study was ranged from 0.137 to 25nM in buffer and from 0.105 to 25nM in spiked human serum, respectively. T PMID: 27916536
Vaspin acts as a coordinator of the dynamic balance between bone formation and resorption processes; its action is affected by the cytokines of the RANKL/RANK/OPG system. Changes in the relationships between vaspin, bone markers, OPG, and RANKL might contribute to the development of osteoporosis in girls with anorexia nervosa. PMID: 28042653
These findings suggest that vaspin and VAP-1 may play a role in the pathogenesis of psoriasis and can be used as markers of the disease; vaspin levels may reflect the severity of disease. PMID: 25279492
Circulating vaspin levels were lower in nonalcoholic fatty liver disease. PMID: 27493109
Circulating vaspin level is higher in women than in men in obese individuals. Vaspin had mediator effect between visceral fat and fat mass associations with resting metabolic rate in obese participants. PMID: 26058881
High serum vaspin concentrations are associated with Obese type 2 diabetes mellitus. PMID: 26676943
The minor allele of vaspin rs2236242 polymorphism plays a protective role against metabolic syndrome. PMID: 26472663
High serum vaspin levels are associated with coronary artery disease in patients with type 2 diabetes. PMID: 26972957
Vaspin might be a useful predictive biomarker in patients with AMI, and patients with low vaspin levels might have a high risk of a MACE. In addition, vaspin might have protective effects on the improvement of LVEF after AMI. PMID: 27322007
High levels of serum vaspin is associated with obesity. PMID: 25470626
Low vaspin blood levels in patients with prolactinomas. PMID: 26415032
The development of clinically manifest arthritis after follow-up was associated with baseline serum vaspin levels. PMID: 26670468
Mean vaspin and apelin levels were significantly lower in underweight children compared to controls PMID: 25915077
serum visceral adipose tissue-derived serpin (vaspin) levels in patients with normal glucose regulation and recently diagnosed type 2 diabetes, was investigated. PMID: 26436376
It is believed that vaspin could be regarded as a new link between obesity and related metabolic disorders, including glucose intolerance. PMID: 25945347
during pregnancy, high vaspin concentration is significantly associated with insulin resistance in gestational diabetes mellitus PMID: 24929806
structural and functional results reveal the mechanistic basis of how reactive centre loop sequence and exosite interaction in vaspin enable KLK7 recognition and regulate protease inhibition PMID: 26199422
Serum vaspin levels showed no significant difference between the type 2 diabetics with renal insufficiency and without renal insufficiency. PMID: 25133192
In the sepsis group, vaspin plasma concentration was about three-fold as high as in the median surgical control group. PMID: 25672472
The expression of human vaspin (serpinA12) is positively correlated to body mass index and insulin sensitivity and increases glucose tolerance in vivo, suggesting a compensatory role in response to diminished insulin signaling in obesity. PMID: 24596079
Higher vaspin levels are associated with obesity and type 2 diabetes mellitus. PMID: 25151227
Vaspin levels have been shown to increase in acute ischemic stroke patients. The increased vaspin levels may vary depending on several factors in acute period of ischemic stroke. PMID: 23594688
the correlations of visfatin/Nampt, vaspin, and RBP-4 with insulin resistance are tissue dependent. PMID: 24367155
serum vaspin level was positively associated with c-IMT, independently of insulin resistance in a general population. PMID: 24529152
vaspin is an adipokine that may have a role in metabolic syndrome in males PMID: 23992351
A rare variant (rs61757459) in vaspin coding for the stop codon p.R211X is related to lower circulating vaspin (SERPINA12) concentrations. Structure analysis suggests misfolding and instability due to the absence of core structural domains. PMID: 23756768
Vaspin rs2236242 gene polymorphism is associated with polycystic ovary syndrome risk. PMID: 24530312
Serum vaspin concentrations are elevated in patients with ulcerative colitis. PMID: 24166663
The plasma vaspin concentrations correlate with the severity of coronary artery disease. PMID: 23850850
Variability in serum vaspin concentrations might be explained by its genetic variants. PMID: 22907691
Low levels of circulating adiponectin and vaspin levels are associated with an increased risk of developing endometrial cancer. PMID: 23896633