Human acetylcholine receptor antibody,AChRab ELISA Kit

Instructions
Code CSB-E08371h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

The Human acetylcholine receptor antibody (AChRab) ELISA kit is used for qualitative identification of AChRab in human serum. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with acetylcholine receptor (AChR). Samples or standards are pipetted into the wells with anti-human IgG conjugated HRP. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of AChRab bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm. The AChRab titer in the samples is determined by referring to the negative control. It indicates the presence of ACHRab if the O.D. (optical density) of AChRab is greater than or equal to the cutoff value (2.1× Average O.D. value of negative control). There is no AChRab present in the sample if the O.D. is less than the cut-off value.

This assay has high sensitivity and excellent specificity for detection of AChRab. And it also has been validated with precision less than 15% and lot-to-lot consistency. Get more details from the product instructions.

AChRab is a protein found in 70-90% of patients with generalized acquired myasthenia gravis (MG), a condition that causes weakness of the eyelids and extraocular muscles. It functions by interfering with acetylcholine, which sends signals from nerves to muscles and between nerves in the brain.

Target Name acetylcholine receptor antibody,AChRab
Abbreviation AChR Ab
Species Homo sapiens (Human)
Sample Types serum
Detection Range Request Information
Sensitivity Request Information
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle qualitative
Measurement Indirect
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Typical Data
             
             
Test parameter specification test result  
Positive control ≥0.6 0.834  
Negative control ≤0.1 0.047  
Positive rate 10,Positive 100%  
Negative rate 10,Negative 100%  
Materials provided
  • A 96-well Assay plate --The 96-well plate has been pre-coated with AChR.
  • Negative Control (1 x 1ml) --Eliminate false positive
  • Positive Control (1 x 1ml) --Used to evaluate the validity, stability, and comparability of experimental results.
  • Sample Diluent (1x 20 ml) --Dilute the sample to an appropriate concentration.
  • HRP-conjugated anti-human IgG (1 x 10ml)--Bind to the AChRab, and HRP catalyzes the TMB to elicit a chromogenic reaction.
  • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
  • Substrate A (1 x 5 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
  • Substrate B (1 x 5 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
  • Stop Solution (1 x 5 ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)--Cover the microplate when incubation.
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

 Q&A
Q:

Under storage section: It has mentioned that opened kit can be stored at 2-8 degrees upto 2 weeks( in case of AcH R) and only upto one month ( in case of MuSk kit)---These are special tests which we can't consume opend kit in 2 weeks or 1 month) minimum it should work 5-6 months( Please confirm how many months we can use opened kit with proper storage and handling of the kit)

A:
Thanks for your inquiry. For the storage condition, pls follow the manual.
Regarding CSB-E08371h, you need to set 6 wells including control,blank wells.Thus the kit can test 90 samples.If you set duplicates,then it can test 45 samples.Based on the existing experiment condition,we suggest you try your best to collect samples as much as possible to avoid any affects to the testing results due to the overdue.
Our elisa kits are only for research use,not for IVD use.There is no industrial standards for kits for research use and we do not have CE certificate.We only have ISO 9001 certificate.
If you need to do seperate assays with one kit during long period,we suggest you order small size,such as 24T or 48T.But you can only order qty 1 for the same item of 24T.
If further questions,pls let me know.

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1