Canine acetylcholine receptor antibody,AChRab ELISA Kit

Instructions
Code CSB-E08375c
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name acetylcholine receptor antibody,AChRab
Abbreviation AChR Ab
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma
Detection Range 3.75 ng/mL-240 ng/mL
Sensitivity 0.94 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine AChRab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 89-102  
1:2 Average % 95  
Range % 84-100  
1:4 Average % 96  
Range % 90-103  
1:8 Average % 94  
Range % 82-101  
Recovery
The recovery of canine AChRab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-101  
EDTA plasma (n=4) 92 87-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
240 2.954 2.918 2.936 2.832  
120 2.512 2.631 2.572 2.468  
60 1.852 1.873 1.863 1.759  
30 1.034 0.996 1.015 0.911  
15 0.472 0.494 0.483 0.379  
7.5 0.261 0.276 0.269 0.165  
3.75 0.155 0.161 0.158 0.054  
0 0.102 0.106 0.104    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

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 Q&A
Q:

I want to use this kit to test the possibility of myasthenia gravis.

A:
Thanks for your inquiry. It is ok to test possibility of myasthenia gravis but we did not ever validate it. Pls follow your actual test result.

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