Human acylation stimulating protein(ASP) ELISA Kit

Instructions
Code CSB-E13474h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name acylation stimulating protein(ASP)
Alternative Names N/A
Abbreviation ASP
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ASP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 97  
Range % 91-103  
1:200 Average % 89  
Range % 84-95  
1:400 Average % 95  
Range % 90-99  
1:800 Average % 84  
Range % 80-88  
Recovery
The recovery of human ASP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 105 100-110  
EDTA plasma (n=4) 90 85-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.527 2.452 2.490 2.390  
5 1.858 1.976 1.917 1.817  
2.5 1.204 1.294 1.249 1.149  
1.25 0.657 0.616 0.637 0.537  
0.625 0.356 0.377 0.367 0.267  
0.312 0.201 0.211 0.206 0.106  
0.156 0.134 0.129 0.132 0.032  
0 0.098 0.101 0.100    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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