Human alpha-actinin-4(ACTN-4) ELISA Kit

Instructions
Code CSB-E13742h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name actinin, alpha 4
Alternative Names actinin 4 ELISA Kit; Actinin alpha 4 ELISA Kit; actinin4 ELISA Kit; ACTN 4 ELISA Kit; ACTN4 ELISA Kit; ACTN4_HUMAN ELISA Kit; Alpha-actinin-4 ELISA Kit; DKFZp686K23158 ELISA Kit; F actin cross linking protein ELISA Kit; F-actin cross-linking protein ELISA Kit; Focal segmental glomerulosclerosis 1 ELISA Kit; FSGS 1 ELISA Kit; FSGS ELISA Kit; FSGS1 ELISA Kit; Non muscle alpha actinin 4 ELISA Kit; Non-muscle alpha-actinin 4 ELISA Kit
Abbreviation ACTN4
Uniprot No. O43707
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ACTN-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 90  
Range % 84-96  
1:200 Average % 90  
Range % 84-95  
1:400 Average % 99  
Range % 95-105  
1:800 Average % 94  
Range % 89-99  
Recovery
The recovery of human ACTN-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-96  
EDTA plasma (n=4) 99 90-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.256 2.298 2.277 2.130  
1000 1.964 1.904 1.934 1.787  
500 1.433 1.467 1.450 1.303  
250 1.021 1.061 1.041 0.894  
125 0.698 0.655 0.677 0.530  
62.5 0.478 0.491 0.485 0.338  
31.25 0.267 0.283 0.275 0.128  
0 0.146 0.148 0.147    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation
Gene References into Functions
  1. we summarize current knowledge regarding therapeutic strategies for locally advanced pancreatic cancer and discuss the potential development of personalized medicine using ACTN4 measurement for patients with locally advanced pancreatic cancer--{REVIEW} PMID: 29921500
  2. Actinin-4 directly binds to the cytosolic tail of CD147 and governs the assembly of CD147-beta2-adrenergic receptor complexes in highly ordered clusters at bacterial adhesion sites.Actinin-4 is recruited to bacterial adhesion sites. PMID: 28569760
  3. significantly upregulated in the cervical mucus of women with preterm labor with microbial invasion of the amniotic cavity PMID: 28743114
  4. Data suggest that TYRO3-mediated phosphorylation of ACTN4 is involved in invasiveness of melanoma cells; TYRO3-mediated phosphorylation of ACTN4 requires FAK activation at tyrosine 397. (TYRO3 = TYRO3 protein tyrosine kinase; ACTN4 = actinin alpha 4; FAK = focal adhesion kinase isoform FAK1) PMID: 29274473
  5. ACTN4 knockdown resulted in the blockage of malignant cell proliferation, colony formation, and ameliorated metastasis potency. ACTN4-positive CSCs exhibited a higher ESA(+) proportion, increased mammosphere-formation ability, and enhanced in vivo tumorigenesis ability. PMID: 29197410
  6. These results demonstrate that both actinin-1 and actinin-4 participate in the assembly of F-actin at invadopodia. Additionally, actinin-4 may have a selective advantage in accelerating invadopodia-mediated invasion of carcinoma cells. PMID: 28797528
  7. Gene amplification of ACTN4 was a significant independent risk factor for death in patients with stage I/II oral tongue cancer (hazard ratio 6.08, 95% confidence interval 1.66-22.27). Gene amplification of ACTN4 is a potential prognostic biomarker for overall survival in oral tongue cancer. PMID: 28385383
  8. ACTN4 knockdown near-completely abrogates both radioresistance and epithelial-mesenchymal transition-associated changes in breast cancer cells. PMID: 29055790
  9. Fascin and alpha-actinin intrinsically segregate to discrete bundled domains that are specifically recognized by other actin-binding proteins. PMID: 27666967
  10. Suggest that ACTN4 as a potential predictive biomarker for efficacy of adjuvant chemotherapy in stage-IB/II patients with non-small cell lung cancer, by reflecting the metastatic potential of tumor cells. PMID: 27121206
  11. High ACTN4 expression is associated with Lung Squamous Cell Carcinoma. PMID: 27613525
  12. Our findings indicate that ACTN4 promotes EMT and tumorigenesis by regulating Snail expression and the Akt pathway in cervical cancer. We propose a novel mechanism for EMT and tumorigenesis in cervical cancer. PMID: 27065319
  13. Study found a functional Validation of an Alpha-Actinin-4 Mutation as a Potential Cause of an Aggressive Presentation of Adolescent Focal Segmental Glomerulosclerosis. PMID: 27977723
  14. SERPINE1, ANGPLT4, CCL20, and SAA1 as well as the NF-kappaB (p65) binding sites on GR-transrepressed promoters such as IL-1beta, IL-6, and IL-8 Taken together, our data establish ACTN4 as a transcriptional co-regulator that modulates both dexamethasone-transactivated and -transrepressed genes in podocytes. PMID: 27998979
  15. Here, we report the identification of a novel, disease-causing ACTN4 mutation (p.G195D, de novo) in a sporadic case of childhood FSGS using next generation sequencing. PMID: 26740551
  16. the calmodulin domain of human alpha-actinin-4 binding to the linker region was important for regulating the distance between actin and actin-binding domain. PMID: 27028653
  17. This study identified alpha-actinin-4 as a novel NHERF1 interaction partner and provided new insights into the regulatory mechanism of the actin cytoskeleton by NHERF1. PMID: 26432781
  18. Selective regulation of NF-kappaB gene targets may indicate involvement of ACTN4 in determining the specificity of cell response to NF-kappaB activation in cells of different types. (Review) PMID: 26995963
  19. ZYX defective binding to ACTN4, which occupies focal adhesions instead of ACTN1, induces the formation of immature focal adhesions, resulting in the enhancement of cell motility and invasion. PMID: 25860875
  20. The contribution of ACTN4 to the process of lung cancer metastasis to the brain could be mainly through regulation of actin cytoskeleton reorganization, cell motility, and focal adhesion. PMID: 25885339
  21. SLK interacts with Tpr and alpha-actinin-4 in cells, and these protein-protein interactions may control the subcellular localization and the biological activity of SLK. PMID: 26094769
  22. key regulator of endothelial cell stiffness and of ICAM-1-mediated neutrophil transmigration PMID: 25107367
  23. Knockdown of ACTN4 protein inhibited cell invasion and migration. These results suggest that ACTN4 is associated with lung cancer cell motility. PMID: 25299231
  24. alpha-actinin-4 is a novel group 1 mGluR-interacting partner that orchestrates spine dynamics and morphogenesis in neurons. PMID: 25944910
  25. Using fluorescence recovery after photobleaching, we show that an ACTN4 mutation that causes human kidney disease roughly triples the wild-type binding affinity of ACTN4 to F-actin in cells, increasing the dissociation time from 29 +/- 13 to 86 +/- 29 s. PMID: 25918384
  26. Estrogen promotes the invasion of ovarian cancer cells via activation of the PI3K/AKT pathway, downregulation of Ecadherin and upregulation of alphaactinin4. PMID: 25216292
  27. ACTN4va expression was also significantly related to distant metastasis PMID: 25750325
  28. The copy number of ACTN4 is a predictive biomarker for Chemoradiotherapy of locally advanced pancreatic cancer. PMID: 25602965
  29. ACTN4 is a transcriptional coactivator of NF-kappaB. PMID: 25411248
  30. The mRNA/protein expressions of ACTN4a was higher in the stenotic colon segment tissue than in the normal colon segment tissue of Hirschsrung disease patients. PMID: 24966941
  31. Findings suggest that alpha-Actinin-4 (ACTN4) plays an important role in maintaining the amoeboidal morphology of invasive melanoma and thus promoting dissemination through collagen-rich matrices. PMID: 25296750
  32. Copy number increase of ACTN4 is a novel indicator for an unfavorable outcome in patients with salivary gland carcinoma. PMID: 24574362
  33. significant correlation between actinin-4 expression and infiltrating glioma grade PMID: 23504125
  34. Actinin-4, a novel interacting partner of influenza A virus nucleoprotein, plays a crucial role in viral replication. PMID: 24802111
  35. 20 exonic and intronic substitutions were found among 48 Czech FSGS/MCD patients. 2242A>G is a candidate mutation in 1 patient. In IGA nephropathy 3 new substitutions were found: c.2351C>T, c.2378G>A and c.2393G>A. PMID: 23890478
  36. High ACTN4 expression is associated with thyroid cancer PMID: 24922688
  37. Increased expression of ACTN4 is associated with invasiveness of bladder cancer. PMID: 23817592
  38. Multivariate analysis revealed that ACTN4 gene amplification was the most significant independent factor associated with an extremely high risk of death among 290 patients with stage I lung adenocarcinoma. PMID: 23899839
  39. Data suggest that in the nucleus, ACTN4 functions as a selective transcriptional co-activator of RelA/p65. PMID: 23482348
  40. Mutations of TRPC6 and ACTN4 occur in only a minor portion of Chinese familial familial focal segmental glomerulosclerosis patients. PMID: 23689571
  41. Data suggest that ACTN4 plays roles in breast cancer tumorigenesis via promotion of cell proliferation, metastasis, and drug resistance. PMID: 23810014
  42. the C-terminal tail of alpha-actinin-4 is essential for its function in cell migration and adhesion to substratum. PMID: 23466492
  43. results demonstrate that monoclonal C-7 antibody exerts an inhibitory effect on endothelium-dependent vasorelaxation induced by acetylcholine and that this response may at least partially result from the inhibition of alpha-actinin 4 PMID: 23711888
  44. Actinin-alpha4 readily forms heterodimers composed of monomers that may have different properties and interacting proteins, thus altering our view of non-muscle actinin function. PMID: 23557398
  45. The tyrosine phosphorylation of alpha-actinin1 at Y12 and alpha-actinin4 at Y265 is critical for dorsal stress fiber establishment, transverse arc maintenance and focal adhesion maturation. PMID: 23454549
  46. MTBP also inhibited ACTN4-mediated F-actin bundling. PMID: 22370640
  47. Depletion of alpha-actinin but not RhoA inhibition causes microtubules-cortex associations during early mitosis PMID: 22825873
  48. In actinin-4-knockdown keratinocytes, focal contact area is increased by 25%, and hemidesmosome proteins are mislocalized. PMID: 23085994
  49. these results suggest that D5(H) of high molecular weight kininogen may modulate cell adhesion and invasion together with actinin-4. PMID: 23000411
  50. there is limited evidence of association of the ACTN4 gene in African-Americans with non-diabetic end-stage renal disease PMID: 22965004

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Involvement in disease Focal segmental glomerulosclerosis 1 (FSGS1)
Subcellular Location Nucleus, Cytoplasm, Cell junction
Protein Families Alpha-actinin family
Tissue Specificity Widely expressed.
Database Links

HGNC: 166

OMIM: 603278

KEGG: hsa:81

STRING: 9606.ENSP00000252699

UniGene: Hs.270291

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