Product Details

Uniprot No.

Target Names

Alternative Names

actinin 4 antibody; Actinin alpha 4 antibody; actinin4 antibody; ACTN 4 antibody; ACTN4 antibody; ACTN4_HUMAN antibody; Alpha-actinin-4 antibody; DKFZp686K23158 antibody; F actin cross linking protein antibody; F-actin cross-linking protein antibody; Focal segmental glomerulosclerosis 1 antibody; FSGS 1 antibody; FSGS antibody; FSGS1 antibody; Non muscle alpha actinin 4 antibody; Non-muscle alpha-actinin 4 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human ACTN4

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8E2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Alpha-actinin-4 (ACTN4) serves as a critical actin-crosslinking protein that plays essential roles in cytoskeletal organization, cell motility, and adhesion dynamics. Beyond its structural functions, ACTN4 has garnered significant research attention due to its involvement in focal segmental glomerulosclerosis (FSGS), where mutations in the ACTN4 gene directly contribute to kidney disease pathology. The protein's dual role in both normal cellular architecture and disease mechanisms makes it a compelling target for researchers investigating cytoskeletal biology, cancer metastasis, and renal pathophysiology.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human ACTN4, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit IgG format provides excellent signal-to-noise characteristics across multiple detection platforms.

Validation studies demonstrate robust performance across diverse applications. Western blot analysis confirms specific detection at the expected 105 kDa molecular weight across an extensive panel of human cell lines including HeLa, U-251MG, A-431, COLO205, PC-3, and Jurkat, as well as the mouse NIH/3T3 line, supporting cross-species reactivity for human and mouse samples. Immunofluorescence staining in HeLa cells reveals clear cytoplasmic localization patterns consistent with ACTN4's known distribution, while flow cytometry validation provides quantitative single-cell analysis capabilities.

This antibody supports researchers investigating cytoskeletal dynamics, epithelial-mesenchymal transition, podocyte biology, and FSGS disease mechanisms, offering the application flexibility needed to address complex biological questions from multiple experimental angles.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.

Gene References into Functions

we summarize current knowledge regarding therapeutic strategies for locally advanced pancreatic cancer and discuss the potential development of personalized medicine using ACTN4 measurement for patients with locally advanced pancreatic cancer--{REVIEW} PMID: 29921500
Actinin-4 directly binds to the cytosolic tail of CD147 and governs the assembly of CD147-beta2-adrenergic receptor complexes in highly ordered clusters at bacterial adhesion sites.Actinin-4 is recruited to bacterial adhesion sites. PMID: 28569760
significantly upregulated in the cervical mucus of women with preterm labor with microbial invasion of the amniotic cavity PMID: 28743114
Data suggest that TYRO3-mediated phosphorylation of ACTN4 is involved in invasiveness of melanoma cells; TYRO3-mediated phosphorylation of ACTN4 requires FAK activation at tyrosine 397. (TYRO3 = TYRO3 protein tyrosine kinase; ACTN4 = actinin alpha 4; FAK = focal adhesion kinase isoform FAK1) PMID: 29274473
ACTN4 knockdown resulted in the blockage of malignant cell proliferation, colony formation, and ameliorated metastasis potency. ACTN4-positive CSCs exhibited a higher ESA(+) proportion, increased mammosphere-formation ability, and enhanced in vivo tumorigenesis ability. PMID: 29197410
These results demonstrate that both actinin-1 and actinin-4 participate in the assembly of F-actin at invadopodia. Additionally, actinin-4 may have a selective advantage in accelerating invadopodia-mediated invasion of carcinoma cells. PMID: 28797528
Gene amplification of ACTN4 was a significant independent risk factor for death in patients with stage I/II oral tongue cancer (hazard ratio 6.08, 95% confidence interval 1.66-22.27). Gene amplification of ACTN4 is a potential prognostic biomarker for overall survival in oral tongue cancer. PMID: 28385383
ACTN4 knockdown near-completely abrogates both radioresistance and epithelial-mesenchymal transition-associated changes in breast cancer cells. PMID: 29055790
Fascin and alpha-actinin intrinsically segregate to discrete bundled domains that are specifically recognized by other actin-binding proteins. PMID: 27666967
Suggest that ACTN4 as a potential predictive biomarker for efficacy of adjuvant chemotherapy in stage-IB/II patients with non-small cell lung cancer, by reflecting the metastatic potential of tumor cells. PMID: 27121206
High ACTN4 expression is associated with Lung Squamous Cell Carcinoma. PMID: 27613525
Our findings indicate that ACTN4 promotes EMT and tumorigenesis by regulating Snail expression and the Akt pathway in cervical cancer. We propose a novel mechanism for EMT and tumorigenesis in cervical cancer. PMID: 27065319
Study found a functional Validation of an Alpha-Actinin-4 Mutation as a Potential Cause of an Aggressive Presentation of Adolescent Focal Segmental Glomerulosclerosis. PMID: 27977723
SERPINE1, ANGPLT4, CCL20, and SAA1 as well as the NF-kappaB (p65) binding sites on GR-transrepressed promoters such as IL-1beta, IL-6, and IL-8 Taken together, our data establish ACTN4 as a transcriptional co-regulator that modulates both dexamethasone-transactivated and -transrepressed genes in podocytes. PMID: 27998979
Here, we report the identification of a novel, disease-causing ACTN4 mutation (p.G195D, de novo) in a sporadic case of childhood FSGS using next generation sequencing. PMID: 26740551
the calmodulin domain of human alpha-actinin-4 binding to the linker region was important for regulating the distance between actin and actin-binding domain. PMID: 27028653
This study identified alpha-actinin-4 as a novel NHERF1 interaction partner and provided new insights into the regulatory mechanism of the actin cytoskeleton by NHERF1. PMID: 26432781
Selective regulation of NF-kappaB gene targets may indicate involvement of ACTN4 in determining the specificity of cell response to NF-kappaB activation in cells of different types. (Review) PMID: 26995963
ZYX defective binding to ACTN4, which occupies focal adhesions instead of ACTN1, induces the formation of immature focal adhesions, resulting in the enhancement of cell motility and invasion. PMID: 25860875
The contribution of ACTN4 to the process of lung cancer metastasis to the brain could be mainly through regulation of actin cytoskeleton reorganization, cell motility, and focal adhesion. PMID: 25885339
SLK interacts with Tpr and alpha-actinin-4 in cells, and these protein-protein interactions may control the subcellular localization and the biological activity of SLK. PMID: 26094769
key regulator of endothelial cell stiffness and of ICAM-1-mediated neutrophil transmigration PMID: 25107367
Knockdown of ACTN4 protein inhibited cell invasion and migration. These results suggest that ACTN4 is associated with lung cancer cell motility. PMID: 25299231
alpha-actinin-4 is a novel group 1 mGluR-interacting partner that orchestrates spine dynamics and morphogenesis in neurons. PMID: 25944910
Using fluorescence recovery after photobleaching, we show that an ACTN4 mutation that causes human kidney disease roughly triples the wild-type binding affinity of ACTN4 to F-actin in cells, increasing the dissociation time from 29 +/- 13 to 86 +/- 29 s. PMID: 25918384
Estrogen promotes the invasion of ovarian cancer cells via activation of the PI3K/AKT pathway, downregulation of Ecadherin and upregulation of alphaactinin4. PMID: 25216292
ACTN4va expression was also significantly related to distant metastasis PMID: 25750325
The copy number of ACTN4 is a predictive biomarker for Chemoradiotherapy of locally advanced pancreatic cancer. PMID: 25602965
ACTN4 is a transcriptional coactivator of NF-kappaB. PMID: 25411248
The mRNA/protein expressions of ACTN4a was higher in the stenotic colon segment tissue than in the normal colon segment tissue of Hirschsrung disease patients. PMID: 24966941
Findings suggest that alpha-Actinin-4 (ACTN4) plays an important role in maintaining the amoeboidal morphology of invasive melanoma and thus promoting dissemination through collagen-rich matrices. PMID: 25296750
Copy number increase of ACTN4 is a novel indicator for an unfavorable outcome in patients with salivary gland carcinoma. PMID: 24574362
significant correlation between actinin-4 expression and infiltrating glioma grade PMID: 23504125
Actinin-4, a novel interacting partner of influenza A virus nucleoprotein, plays a crucial role in viral replication. PMID: 24802111
20 exonic and intronic substitutions were found among 48 Czech FSGS/MCD patients. 2242A>G is a candidate mutation in 1 patient. In IGA nephropathy 3 new substitutions were found: c.2351C>T, c.2378G>A and c.2393G>A. PMID: 23890478
High ACTN4 expression is associated with thyroid cancer PMID: 24922688
Increased expression of ACTN4 is associated with invasiveness of bladder cancer. PMID: 23817592
Multivariate analysis revealed that ACTN4 gene amplification was the most significant independent factor associated with an extremely high risk of death among 290 patients with stage I lung adenocarcinoma. PMID: 23899839
Data suggest that in the nucleus, ACTN4 functions as a selective transcriptional co-activator of RelA/p65. PMID: 23482348
Mutations of TRPC6 and ACTN4 occur in only a minor portion of Chinese familial familial focal segmental glomerulosclerosis patients. PMID: 23689571
Data suggest that ACTN4 plays roles in breast cancer tumorigenesis via promotion of cell proliferation, metastasis, and drug resistance. PMID: 23810014
the C-terminal tail of alpha-actinin-4 is essential for its function in cell migration and adhesion to substratum. PMID: 23466492
results demonstrate that monoclonal C-7 antibody exerts an inhibitory effect on endothelium-dependent vasorelaxation induced by acetylcholine and that this response may at least partially result from the inhibition of alpha-actinin 4 PMID: 23711888
Actinin-alpha4 readily forms heterodimers composed of monomers that may have different properties and interacting proteins, thus altering our view of non-muscle actinin function. PMID: 23557398
The tyrosine phosphorylation of alpha-actinin1 at Y12 and alpha-actinin4 at Y265 is critical for dorsal stress fiber establishment, transverse arc maintenance and focal adhesion maturation. PMID: 23454549
MTBP also inhibited ACTN4-mediated F-actin bundling. PMID: 22370640
Depletion of alpha-actinin but not RhoA inhibition causes microtubules-cortex associations during early mitosis PMID: 22825873
In actinin-4-knockdown keratinocytes, focal contact area is increased by 25%, and hemidesmosome proteins are mislocalized. PMID: 23085994
these results suggest that D5(H) of high molecular weight kininogen may modulate cell adhesion and invasion together with actinin-4. PMID: 23000411
there is limited evidence of association of the ACTN4 gene in African-Americans with non-diabetic end-stage renal disease PMID: 22965004

Hide All

Involvement in disease

Focal segmental glomerulosclerosis 1 (FSGS1)

Subcellular Location

Nucleus. Cytoplasm. Cell junction. Cytoplasm, cytoskeleton, stress fiber. Cytoplasm, perinuclear region.

Protein Families

Alpha-actinin family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 166

OMIM: 603278

KEGG: hsa:81

STRING: 9606.ENSP00000252699

UniGene: Hs.270291

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Code	CSB-RA103439A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HeLa whole cell lysate(30µg), U-251MG whole cell lysate(30µg), A-431 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), Jurkat whole cell lysate(30µg) All lanes: ACTN4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 1min Immunofluorescence staining of Hela cell with CSB-RA103439A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA103439A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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ACTN4 Recombinant Monoclonal Antibody

Product Details

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Target Background