Human alzheimer-associated neuronal thread protein (AD7C-NTP) ELISA kit

Code CSB-EQ027774HU
See More Details 24T ELISA kits trial application
Product Type ELISA kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation AD7C-NTP
Protein Biological Process 1 others
Target Name alzheimer-associated neuronal thread protein (AD7C-NTP)
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 23.43 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human AD7C-NTP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:20Average %97
Range %93-101
1:40Average %93
Range %86-97
1:80Average %93
Range %87-97
1:160Average %102
Range %98-106
Recovery
The recovery of Human AD7C-NTP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9894-102
EDTA plasma (n=4)8782-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
60002.615 2.787 2.701 2.614
30002.214 2.115 2.165 2.078
15001.441 1.386 1.414 1.327
7500.784 0.821 0.803 0.716
3750.466 0.457 0.462 0.375
187.50.274 0.286 0.280 0.193
93.750.172 0.166 0.169 0.082
00.086 0.087 0.087
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