Human alzheimer-associated neuronal thread protein (AD7C-NTP) ELISA kit

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Human alzheimer-associated neuronal thread protein (AD7C-NTP) ELISA kit

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Protocol MSDS

Code	CSB-EQ027774HU
Size	96T,5×96T,10×96T
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Product Details
Customer Reviews and Q&A

Target Name

alzheimer-associated neuronal thread protein (AD7C-NTP)

Abbreviation

AD7C-NTP

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

93.75 pg/mL-6000 pg/mL

Sensitivity

23.43 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Human AD7C-NTP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	97
	Range %	93-101
1:40	Average %	93
	Range %	86-97
1:80	Average %	93
	Range %	87-97
1:160	Average %	102
	Range %	98-106

Recovery

The recovery of Human AD7C-NTP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	87	82-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	2.615	2.787	2.701	2.614
3000	2.214	2.115	2.165	2.078
1500	1.441	1.386	1.414	1.327
750	0.784	0.821	0.803	0.716
375	0.466	0.457	0.462	0.375
187.5	0.274	0.286	0.280	0.193
93.75	0.172	0.166	0.169	0.082
0	0.086	0.087	0.087

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human AD7C-NTP ELISA Kit was designed for the quantitative measurement of Human AD7C-NTP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 pg/mL-6000 pg/mL and the sensitivity is 23.43 pg/mL.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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