Human angiotensin Ⅱ (ANG-Ⅱ) ELISA Kit

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Name: Human angiotensin Ⅱ (ANG-Ⅱ) ELISA Kit
Brand: CUSABIO
SKU: CSB-E04493h

Alternative Names

angiotensin Ⅱ, angiotensin 2, ANG2

Abbreviation

ANG-Ⅱ

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

31.25 pg/ml-2000 pg/ml.

Sensitivity

15.6 pg/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ANG- Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-101
1:2	Average %	89
	Range %	82-95
1:4	Average %	93
	Range %	88-99
1:8	Average %	96
	Range %	89-103

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

2000

0.159

0.154

0.157

1000

0.214

0.222

0.218

500

0.292

0.286

0.289

250

0.370

0.398

0.384

125

0.568

0.561

0.565

62.5

0.915

0.933

0.924

31.25

1.346

1.284

1.315

0

2.207

2.125

2.166

Materials provided

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ANG-II antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ANG-II antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

Angiotensin II is a potent vasoconstrictor peptide hormone that plays a central role in the renin-angiotensin-aldosterone system, which regulates blood pressure, fluid balance, and cardiovascular homeostasis. This octapeptide forms through the enzymatic cleavage of angiotensin I by angiotensin-converting enzyme and exerts its effects primarily through binding to angiotensin II type 1 and type 2 receptors. Research into angiotensin II levels is essential for understanding hypertension, heart failure, kidney disease, and other cardiovascular disorders. This makes it an important biomarker in clinical and research settings.

The Human angiotensin II ELISA Kit (CSB-E04493h) provides quantitative measurement of ANG-II in human serum, plasma, cell culture supernates, and tissue homogenates using a sandwich assay principle. The kit offers a detection range of 31.25 pg/mL to 2000 pg/mL with a sensitivity of 7.81 pg/mL, requiring 50-100 μL sample volume per well. Detection occurs at 450 nm wavelength. Assay completion takes 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research focused on measuring angiotensin II levels in cell culture systems. The applications show its use in studies examining the renin-angiotensin system and related physiological processes.

• Cell culture research: Quantification of angiotensin II in cell culture supernatants to assess cellular production and secretion patterns
• Renin-angiotensin system studies: Analysis of angiotensin II levels as part of broader research into cardiovascular and metabolic regulatory pathways
• Biomarker analysis: Measurement of angiotensin II concentrations for correlation with physiological responses and experimental conditions

Code	CSB-E04493h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<12%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.