Code | CSB-E04494r |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<12% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of rat ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 84 | ||||
Range % | 80-92 | |||||
1:2 | Average % | 87 | ||||
Range % | 81-95 | |||||
1:4 | Average % | 90 | ||||
Range % | 92-97 | |||||
1:8 | Average % | 93 | ||||
Range % | 86-98 |
The recovery of rat ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 86-98 | ||||
EDTA plasma (n=4) | 87 | 82-95 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/ml | OD1 | OD2 | Average | ||||
1000 | 0.158 | 0.160 | 0.159 | ||||
500 | 0.185 | 0.189 | 0.187 | ||||
250 | 0.237 | 0.232 | 0.235 | ||||
125 | 0.364 | 0.340 | 0.352 | ||||
62.5 | 0.595 | 0.568 | 0.581 | ||||
31.25 | 0.958 | 0.902 | 0.930 | ||||
15.6 | 1.355 | 1.298 | 1.326 | ||||
0 | 2.287 | 2.126 | 2.206 |
This Rat ANG-Ⅱ ELISA Kit was designed for the quantitative measurement of Rat ANG-Ⅱ protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit.
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