Rat angiotensin II (ANG-Ⅱ) ELISA kit

Code CSB-E04494r
Size 96T,5×96T,10×96T
Price Request a Quote
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Alternative Names
angiotensin II,ANG-Ⅱ,angiotensin 2,ANG-2
Abbreviation
ANG-Ⅱ
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma.
Detection Range
15.6 pg/ml-1000 pg/ml.
Sensitivity
7.8 pg/ml.
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision

Intra-assay Precision (Precision within an assay): CV%<12%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Sample

Serum(n=4)

1:1

Average %

84

Range %

80-92

1:2

Average %

87

Range %

81-95

1:4

Average %

90

Range %

92-97

1:8

Average %

93

Range %

86-98

Recovery

The recovery of rat ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

92

86-98

EDTA plasma (n=4)

87

82-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

1000

0.158

0.160

0.159

500

0.185

0.189

0.187

250

0.237

0.232

0.235

125

0.364

0.340

0.352

62.5

0.595

0.568

0.581

31.25

0.958

0.902

0.930

15.6

1.355

1.298

1.326

0

2.287

2.126

2.206

Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Rat ANG-Ⅱ ELISA Kit was designed for the quantitative measurement of Rat ANG-Ⅱ protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit. 

Citations

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