Human asymmetrical dimethylarginine,ADMA ELISA Kit

Instructions
Code CSB-E09298h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name asymmetrical dimethylarginine,ADMA
Abbreviation ADMA
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 7.8 ng/mL-500 ng/mL
Sensitivity 1.95 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ADMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %99
Range %94-105
1:2Average %94
Range %90-98
1:4Average %95
Range %91-100
1:8Average %93
Range %89-97
Recovery
The recovery of human ADMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-94
EDTA plasma (n=4)8782-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
5002.763 2.612 2.688 2.552
2502.249 2.149 2.199 2.063
1251.467 1.428 1.448 1.312
62.50.883 0.823 0.853 0.717
31.20.497 0.505 0.501 0.365
15.60.357 0.386 0.372 0.236
7.80.241 0.229 0.235 0.099
00.139 0.132 0.136  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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