Human cancer antigen 27-29 (CA 27-29) ELISA Kit

Instructions
Code CSB-E13858h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cancer antigen 27-29 (CA 27-29)
Abbreviation CA 27-29
Species Homo sapiens (Human)
Sample Types serum
Detection Range 3 U/mL-100 U/mL
Sensitivity 0.8 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<20%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CA 27-29 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %80-92
1:2Average %96
Range %91-103
1:4Average %97
Range %90-105
1:8Average %92
Range %85-98
Recovery
The recovery of human CA 27-29 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/mlOD1OD2Average
1001.375 1.394 1.385
500.930 0.945 0.938
170.465 0.449 0.457
30.190 0.193 0.192
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

I plan to use plasma samples with this kit and would like to know ifwe can use EDTA purple topped phlebotomy tubes to collect the samples.
I found the following link which seems to fit her description.
http://www.austincc.edu/kotrla/phb_purple
I am fairly certain that EDTA tubes can be used but please can you confirm?

A:
Thanks for your inquiry.
CSB-E13858h
We suggest you test serum samples. The detection effect is not good if you test plasma samples.
The concentration in plasma samples may be very low or the fibrinogen in the plasma may interfere with the detection.
Pls let me know if you have any further questions. Thank you.

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