Human casein alpha s1 (CSN1S1) ELISA kit

Code CSB-EL006060HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
casein alpha s1
Alternative Names
Alpha casein ELISA Kit; alpha-S1-casein ELISA Kit; CASA ELISA Kit; CASA1_HUMAN ELISA Kit; Casein alpha s1 ELISA Kit; casein; alpha ELISA Kit; casein; alpha-S1; included ELISA Kit; Casoxin-D ELISA Kit; CSN1 ELISA Kit; CSN1S1 ELISA Kit; Csna ELISA Kit; MGC149368 ELISA Kit
Abbreviation
CSN1S1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
breast milk
Detection Range
37.5 μg/mL-600 μg/mL
Sensitivity
18.75 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CSN1S1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleBreast milk(n=4)
1:10Average %90
Range %86-97
1:20Average %93
Range %89-99
1:40Average %95
Range %90-107
1:80Average %92
Range %87-103
Recovery
The recovery of human CSN1S1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Breast milk (n=5) 9587-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2Average
6000.215 0.233 0.224
3000.349 0.374 0.362
1500.655 0.612 0.634
751.358 1.326 1.342
37.51.764 1.727 1.746
02.631 2.419 2.525
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CSN1S1 ELISA Kit was designed for the quantitative measurement of Human CSN1S1 protein in breast milk. It is a Competitive ELISA kit, its detection range is 37.5 μg/mL-600 μg/mL and the sensitivity is 18.75 μg/mL.

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Target Background

Function
(From Uniprot)
Important role in the capacity of milk to transport calcium phosphate.; Casoxin D acts as opioid antagonist and has vasorelaxing activity mediated by bradykinin B1 receptors.
Gene References into Functions
  1. Serine-71 in alpha-S1 casein is phosphorylated in the breast milk. PMID: 28786558
  2. Results suggest that autoimmunity to insulin can occur infrequently via cross-reactivity to food protein alpha casein. PMID: 21361910
  3. Casein alpha s1 is expressed in human monocytes and stimulates expression of proinflammatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF) via an immunomodulatory role beyond the nutritional function of CSN1S1 in milk. PMID: 21135174
  4. alphas1-Casein may be a potential biomarker for early identification of BPH patients. PMID: 16683014
Subcellular Location
Secreted.
Protein Families
Alpha-casein family
Tissue Specificity
Mammary gland specific. Secreted in milk.
Database Links

HGNC: 2445

OMIM: 115450

KEGG: hsa:1446

STRING: 9606.ENSP00000246891

UniGene: Hs.3155

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