Human colon cancer-specific antigen-2,CCSA-2 ELISA kit

Code CSB-E11291h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
colon cancer-specific antigen-2,CCSA-2
Abbreviation
CCSA-2
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
3.33 ng/mL-80 ng/mL
Sensitivity
1.33 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<10%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CCSA-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-100
1:2Average %92
Range %88-100
1:4Average %98
Range %92-106
1:8Average %95
Range %86-101
Recovery
The recovery of human CCSA-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9689-102
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
802.015 1.970 1.993 1.946
401.344 1.358 1.351 1.304
200.799 0.801 0.800 0.753
6.670.341 0.359 0.350 0.303
3.330.201 0.215 0.208 0.161
00.047 0.047 0.047  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CCSA-2 ELISA Kit was designed for the quantitative measurement of Human CCSA-2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.33 ng/mL-80 ng/mL and the sensitivity is 1.33 ng/mL .

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