Human colon cancer-specific antigen-2,CCSA-2 ELISA kit

Instructions
Code CSB-E11291h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name colon cancer-specific antigen-2,CCSA-2
Abbreviation CCSA-2
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 3.33 ng/mL-80 ng/mL
Sensitivity 1.33 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<10%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CCSA-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-100
1:2Average %92
Range %88-100
1:4Average %98
Range %92-106
1:8Average %95
Range %86-101
Recovery
The recovery of human CCSA-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9689-102
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
802.015 1.970 1.993 1.946
401.344 1.358 1.351 1.304
200.799 0.801 0.800 0.753
6.670.341 0.359 0.350 0.303
3.330.201 0.215 0.208 0.161
00.047 0.047 0.047  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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