Human heparan sulfate,HS ELISA Kit

Instructions
Code CSB-E09585h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

The Human heparan sulfate (HS) ELISA Kit is applied to detect and quantify the concentrations of HS in serum, plasma, urine, and tissue homogenates. This kit exclusively recognizes human HS protein. This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with an HS antigen. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for HS and HRP-conjugated goat-anti-mouse HS antibody. The competitive inhibition reaction is launched between pre-coated HS and HS in samples or samples. A substrate solution is added to the wells and The intensity of the color is negatively relevant to the levels of HS in the sample. The color development is stopped and the intensity of the color is measured.

This ELISA kit has been confirmed to have high sensitivity, excellent specificity, premium precision, and lot-to-lot consistency. See the product instructions for more details.

Heparan sulfate (HS) is a highly negatively charged linear polysaccharide and commonly binds to a small set of the membrane or extracellular matrix proteoglycansa to form heparan sulfate proteoglycans (HSPGs). It is a ubiquitous compound of animal cells. Both HS and HSPGs binds to protein ligands and act as co-receptors for different cell surface receptors, regulating a broad range of biological activities, such as developmental processes, angiogenesis, cell adhesion, immune cell infiltration, blood coagulation, abolishing detachment activity by GrB (Granzyme B), and tumor metastasis. Evidence has demonstrated that HS is deregulated in a variety of solid tumors and hematological malignancies and modulate key aspects of cancer initiation and progression. Additionally, numerous viruses (including emerging SARS-CoV-2) and parasites use cell surface heparan sulfate as receptors to infect target cells.

Target Name heparan sulfate,HS
Alternative Names N/A
Abbreviation HS
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, tissue homogenates
Detection Range 31.25 ng/mL-8000 ng/mL
Sensitivity 31.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Competitive
Precision  
Linearity  
Recovery  
Typical Data  
Materials provided
  • A 96-well Assay plate --The 96-well plate has been pre-coated with HS.
  • Standard (2 x 250 μl)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
  • Anti-human HS Antibody (100 x concentrate) (1 x 60 μl)
  • Antibody Diluent (1 x 10 ml) --Dilute the anti-human LRG1
  • HRP-conjugated goat-anti-mouse HS antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
  • HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated HS antibody.
  • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
  • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
  • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

 Q&A
Q:

What does Cusabio recommend for sample dilution buffer? None is supplied in the kit and I want to know what should be diluting my samples with to be compatible with the cusabio ELISA kit. (CSB-E09585h)
Is it distilled water or some other buffer?

A:
Thanks for your inquiry. We recommend you use normal saline or PBS.

  Tel: 301-363-4651
  Email: support@cusabio.com
  Distributors Worldwide

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1