Human histatin 5 ELISA Kit

Instructions
Code CSB-E09935h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name histatin 5
Alternative Names N/A
Abbreviation histatin-5
Species Homo sapiens (Human)
Sample Types serum, plasma, saliva
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Biochemicals
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Histatin 5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 84-95  
1:2 Average % 94  
Range % 90-98  
1:4 Average % 100  
Range % 95-104  
1:8 Average % 102  
Range % 96-108  
Recovery
The recovery of human Histatin 5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 97-103  
EDTA plasma (n=4) 95 91-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
800 2.448 2.454 2.451 2.260  
400 2.024 2.167 2.096 1.905  
200 1.629 1.678 1.654 1.463  
100 1.151 1.169 1.160 0.969  
50 0.622 0.634 0.628 0.437  
25 0.375 0.391 0.383 0.192  
12.5 0.312 0.324 0.318 0.127  
0 0.192 0.190 0.191    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Most popular with customers

  Tel: 301-363-4651
  Email: support@cusabio.com
  Distributors Worldwide

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.