Human hypoxia-inducible factor 1α(HIF-1α) ELISA kit

Code CSB-E12112h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q16665
Lead Time 3-5 working days
Abbreviation HIF-1α
Protein Biological Process 1 Transcription/Transcription regulation
Target Name hypoxia-inducible factor 1α(HIF-1α)
Alias HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, ARNT interacting protein|hypoxia-inducible factor 1 alpha isoform I.3|hypoxia-inducible factor 1, alpha subunit|hypoxia-inducible factor 1, alpha
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.
HGNC 4910
RGD 61928
MGI 106918
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 84-96  
1:2 Average % 96  
Range % 90-101  
1:4 Average % 99  
Range % 95-103  
1:8 Average % 94  
Range % 88-98  
Recovery
The recovery of human HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 91-98  
EDTA plasma (n=4) 96 92-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.737 2.718 2.728 2.619  
2000 1.952 1.887 1.920 1.811  
1000 1.342 1.331 1.337 1.228  
500 0.776 0.767 0.772 0.663  
250 0.407 0.399 0.403 0.294  
125 0.309 0.302 0.306 0.197  
62.5 0.195 0.188 0.192 0.083  
0 0.111 0.107 0.109    
References
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Function Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Subcellular Location Cytoplasm, Nucleus, Nucleus speckle
Tissue Specificity Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumo
Database Links

HGNC: 4910

OMIM: 603348

KEGG: hsa:3091

STRING: 9606.ENSP00000338018

UniGene: Hs.597216

Pathway HIF-1 signaling pathway
Autophagy
Th17 cell differentiation

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