Human hypoxia-inducible factor 1α(HIF-1α) ELISA kit

Instructions
Code CSB-E12112h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name hypoxia-inducible factor 1α(HIF-1α)
Alternative Names ARNT interacting protein ELISA Kit; ARNT-interacting protein ELISA Kit; Basic helix loop helix PAS protein MOP1 ELISA Kit; Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit; bHLHe78 ELISA Kit; Class E basic helix-loop-helix protein 78 ELISA Kit; HIF 1A ELISA Kit; HIF 1alpha ELISA Kit; HIF-1-alpha ELISA Kit; HIF-1alpha ELISA Kit; HIF-alpha ELISA Kit; HIF1 A ELISA Kit; HIF1 Alpha ELISA Kit; HIF1 ELISA Kit; HIF1-alpha ELISA Kit; HIF1A ELISA Kit; HIF1A_HUMAN ELISA Kit; hifla ELISA Kit; Hypoxia inducible factor 1 alpha ELISA Kit; Hypoxia inducible factor 1 alpha isoform I.3 ELISA Kit; Hypoxia inducible factor 1 alpha subunit ELISA Kit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor ELISA Kit; Hypoxia inducible factor 1; alpha subunit (basic helix loop helix transcription factor) ELISA Kit; Hypoxia inducible factor1alpha ELISA Kit; Hypoxia-inducible factor 1-alpha ELISA Kit; Member of PAS protein 1 ELISA Kit; Member of PAS superfamily 1 ELISA Kit; Member of the PAS Superfamily 1 ELISA Kit; MOP 1 ELISA Kit; MOP1 ELISA Kit; PAS domain-containing protein 8 ELISA Kit; PASD 8 ELISA Kit; PASD8 ELISA Kit
Abbreviation HIF-1α
Uniprot No. Q16665
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 84-96  
1:2 Average % 96  
Range % 90-101  
1:4 Average % 99  
Range % 95-103  
1:8 Average % 94  
Range % 88-98  
Recovery
The recovery of human HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 91-98  
EDTA plasma (n=4) 96 92-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.737 2.718 2.728 2.619  
2000 1.952 1.887 1.920 1.811  
1000 1.342 1.331 1.337 1.228  
500 0.776 0.767 0.772 0.663  
250 0.407 0.399 0.403 0.294  
125 0.309 0.302 0.306 0.197  
62.5 0.195 0.188 0.192 0.083  
0 0.111 0.107 0.109    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Gene References into Functions
  1. These findings demonstrated a valuable antitumor synergism in combining CRISPR/Cas9mediated HIF1alpha knockout with Transarterial embolization (TAE)in mice and highlighted the possibility that HIF1a may be an effective therapeutic knockout target in combination with TAE for hepatocellular carcinoma treatment PMID: 30226584
  2. Since the FIH-1 dependent hydroxylation of NAA10 occurs oxygen-dependently, NAA10 acetylates HIF-1alpha under normoxia but does not under hypoxia. PMID: 30237125
  3. HIF1A is upregulated in breast and bladder tumors with high NRF2 activity. NRF2 targets a functional antioxidant response element at the HIF1A locus, which reveals a direct regulatory connection between two important oxygen responsive transcription factors. PMID: 30241031
  4. CPT1A is repressed by HIF1 and HIF2, reducing fatty acid transport into the mitochondria, and forcing fatty acids to lipid droplets for storage. PMID: 29176561
  5. MiR-497 approximately 195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch1 and HIF1A activity. PMID: 28685750
  6. Study revealed that HIF1alpha was significantly upregulated in gallbladder cancer (GBC) tissues. HIF1alpha overexpression was closely associated with lymph node metastasis and TNM stage. HIF1alpha was able to promote cell migration in a hypoxic microenvironment by overexpressing VEGF in GBC cell line. PMID: 30272364
  7. HIF-1alpha "positive" had decreased overall survival compared to HIF-1alpha "negative" patients and this was an independent adverse prognostic factor for HCC patients with cirrhosis, but not for cirrhosis-free patients. PMID: 30274686
  8. genetic polymorphisms in HIF1A were not associated with persistent apical periodontitis PMID: 29898065
  9. Hypoxia-induced angiogenesis is a complex process that involves distinct but also overlapping functions of HIF-1alpha and HIF- 2alpha in regard to angiogenesis, bioenergetic adaption and the redundant transcriptional induction of MIF. PMID: 28993199
  10. High HIF1A expression is associated with high Collagen I Fibers in Triple Negative Breast Cancer. PMID: 29247885
  11. Cathepsin B (CTSB) is a novel target gene of hypoxia-inducible factor-1-alpha (HIF-1alpha). CTSB mRNA and protein levels can be up-regulated in a HIF-1alpha-dependent manner. PMID: 29935187
  12. this is the first case-control study uncovering that the HIF1A gene rs10873142 polymorphism increases the risk of COPD in a Chinese Han population. PMID: 29339421
  13. ST6Gal-I activity augmented HIF-1alpha accumulation in cells grown in a hypoxic environment or treated with two chemical hypoxia mimetics, deferoxamine and dimethyloxalylglycine. PMID: 29475939
  14. mRNA expression levels of both HIF1A and LACC1 were upregulated in the skin lesions of individuals with leprosy. PMID: 29706348
  15. data suggest an important role of miR-210 in sustaining HIF-1alpha activity via the suppression of HIF-3alpha, regulating cell growth and chemotherapeutic drug resistance in cholangiocarcinoma. PMID: 29953500
  16. HOXA9 inhibits HIF1A-mediated glycolysis through interacting with CRIP2 to repress cutaneous squamous cell carcinoma development. PMID: 29662084
  17. HIF-1alpha inhibits NCR1/NKp46 pathway through up-regulating miR-224, which affects the killing capability of NK cells on prostate cancer, thus inducing immune escape of tumor cells. PMID: 29885835
  18. The findings demonstrate that AGPAT2, which is mutated in patients with congenital generalized lipodystrophy and over-expressed in different types of cancer, is a direct transcriptional target of HIF-1, suggesting that upregulation of lipid storage by HIF-1 plays an important role in adaptation and survival of cancer cells under low oxygen conditions. PMID: 29908837
  19. Overexpression of HIF1A leads to radioresistance of cervical cancer. PMID: 30355300
  20. Knockdown of DEC2 resulted in a significant (26.7%) reduction of VEGF expression in MIO-M1 cells under hypoxia-mimicking conditions induced by DFO (P < .05). Levels of HIF1alpha protein were also reduced significantly, by 60.2%, in MIO-M1 cells treated with siRNA against the DEC2 gene (P < .05). Moreover, HIF1alpha levels showed a significant (2.5-fold) increase in MIO-M1 cells overexpressing DEC2 (P < .05). PMID: 30250985
  21. High HIF1A expression is associated with salivary adenoid cystic carcinoma. PMID: 30015895
  22. we found that Hif-1alpha directly promoted H19 expression through binding to the H19 promoter and indirectly through SP1-mediated H19 transcriptional activation under hypoxia in glioblastoma cells. PMID: 28327666
  23. The IRIS-driven metastatic mechanism results from IRIS-dependent suppression of phosphatase and tensin homolog (PTEN) transcription, which in turn perturbs the PI3K/AKT/GSK-3beta pathway leading to prolyl hydroxylase-independent HIF-1alpha stabilization and activation in a normoxic environment. PMID: 30254159
  24. Data show that von Hippel-Lindau-binding protein 1 (VBP1) enhances the stability of von Hippel-Lindau tumor suppressor protein (pVHL) and facilitates pVHL-mediated ubiquitination of hypoxia-inducible factor 1, alpha subunit (HIF-1alpha). PMID: 29121446
  25. Multifunctional proteins epigenetically modulating HIF1A stability and activity have been described. (Review) PMID: 29032501
  26. Studies have shown that both HIF1alpha and HIF2alpha may contribute to the regulation of cellular adaptation to hypoxia and resistance to cancer therapies with their potential to exert significant effects on the maintenance and evolution of cancer stem cells. Also, HIF1alpha and HIF2alpha seemed to have significant prognostic and predictive value. [review] PMID: 29845228
  27. HIF-1 was overexpressed in osteosarcoma tissues and cell lines, which promoted cell proliferation, clone formation, migration, invasion and inhibited cell apoptosis. PMID: 29807229
  28. hypoxiainduced expression of CXCR4 promoted trophoblast cell migration and invasion via the activation of HIF1alpha, which is crucial during placentation. PMID: 29786753
  29. Data suggest that NRF2/NFE2L2 promotes breast cancer progression by enhancing glycolysis through co-activation of HIF1A; NRF2 and HIF1A mRNA and protein levels are significantly up-regulated in breast cancer cells as compared to benign breast epithelial cells. (NRF2/NFE2L2 = nuclear factor erythroid 2-related factor 2; HIF1A = hypoxia inducible factor 1 subunit alpha) PMID: 29275212
  30. the expression of MMIF, HIF-1alpha, and VEGF in the serum and endometrial tissues may be used to assess the stage of endometriosis and the severity of dysmenorrhea. PMID: 30074218
  31. Findings revealed an HIF-1alpha/IL-1beta signaling loop between cancer cells and tumor-associated macrophages in a hypoxic microenvironment, resulting in cancer cell epithelial-mesenchymal transition and metastasis; more importantly, our results suggest a potential role of an anti-inflammatory strategy in hepatocellular carcinoma treatment PMID: 29171040
  32. Overexpression of HIF-1alpha and P4HB is associated with poor prognosis in patients with gastric cancer. PMID: 29904245
  33. hypoxic stress in the hepatocellular carcinoma (HCC)cells promoted YAP binding to HIF-1a in the nucleus and sustained HIF-1a protein stability to bind to PKM2 gene and directly activates PKM2 transcription to accelerate glycolysis PMID: 30180863
  34. Overexpression of VHL was more successful at inhibiting fibrosis compared with silencing HIF-1a plus HIF-2a. Normoxia-active HIF-1a or HIF-2a prevented the inhibitory effect of VHL on liver fibrosis, indicating that attenuating fibrosis via VHL is HIF-1a- and HIF-2a-dependent to some extent. PMID: 28112200
  35. Results find that knockdown of HIF-1alpha reduced hypoxia-induced SENP1 expression, suggesting that induction of SENP1 expression is mediated by hypoxia-inducible factor HIF-1alpha. PMID: 28796315
  36. ADM was an upstream molecule of HIF-1alpha/VEGF and it promoted angiogenesis through upregulating HIF-1alpha/VEGF in epithelial ovarian cancer. PMID: 28091613
  37. HIF-1alpha expression correlates with the expression level of IL- 8, as evidenced by the down regulation of IL-8 in response to silencing of HIF-1alpha in HCC cell lines under hypoxic conditions. PMID: 29881400
  38. MOLP8/R cells display a very high overexpression of LCP1 gene (l-Plastin) controlled by HIF1&alpha. PMID: 29882856
  39. the role of FTH1 in the FIH control of HIF-1 activity, is reported. PMID: 29580991
  40. The findings of the current study demonstrate presence of the IDH1 R132H mutation in primary human glioblastoma cell lines with upregulated HIF-1alpha expression, downregulating c-MYC activity and resulting in a consequential decrease in miR-20a, which is responsible for cell proliferation and resistance to standard temozolomide treatment. PMID: 29625108
  41. The cross-talk between HIF1A, NRF2 and NF-kappaB is required to adapt to changes in oxygen availability. (Review) PMID: 29485192
  42. PKD1 not only regulates the hypoxic glycolytic metabolism of cancer cells via regulation of the expression of HIF-1alpha and glycolytic enzymes. PMID: 29901206
  43. Results provide evidence that HIF1alpha overexpression promotes the radioresistance of prostate cancer cells through enhanced betacatenin nuclear translocation. PMID: 29658569
  44. Collectively, the present study demonstrated mitochondrial fission as a tumor suppression process that is regulated by the HIF/miR125a/Mfn2 pathways, acting to restrict PANC1 cell survival, energy metabolism and migration, with potential implications for novel approaches for PC therapy. PMID: 29749475
  45. the results of the present study demonstrated that hypoxia-induced cytoprotective autophagy counteracted gemcitabine-induced apoptosis through increasing HIF1alpha expression PMID: 29693166
  46. CD40 is a key molecule for the upregulation of HIF-1alpha and PTEN underlying the severity of microangiopathy. PMID: 29549140
  47. Enhanced expression of HIF-1alpha may be related to autophagy activation in SH-SY5Y cells, thus contributing to ischemic/hypoxic brain damage. PMID: 29724989
  48. Data show that deletion of hypoxia inducible factor 1 subunit alpha (HIF-1alpha) in NK cells inhibited tumour growth despite impaired tumour cell killing. PMID: 29150606
  49. AEG-1 was found to be significantly associated with hypoxia in ovarian cancer by regulating the HIF-1alpha/NF-kappaB/VEGF pathway. PMID: 29770329
  50. no significant differences in the serum levels between early-onset pre-eclampsia, late-onset pre-eclampsia, and controls PMID: 28574293
  51. The polymorphic HIF-1A rs12434439 GG genotype may play a protective role for rheumatoid arthritis development PMID: 29411043
  52. Results indicate that there is a dysregulation of DMT1 + IRE in IA testes, which might due to the up-regulation of IRP1 and HIF-1A. PMID: 28762519
  53. Results indicated that HIF1alphamediated dysregulation of aerobic glycolysis may be involved in the miR186mediated suppressive effects on osteosarcoma cells. PMID: 29693191
  54. transcriptional activation of the miR-191 promoter by HIF-2alpha is involved in EMT and in the acquisition of a stem cell-like phenotype PMID: 29277653
  55. Parkin expression is inversely correlated with HIF-1alpha expression and metastasis in breast cancer. Our results reveal an important mechanism for Parkin in tumor suppression and HIF-1alpha regulation. PMID: 29180628
  56. Difference in expression of HIF-1alpha in stromal cells of the vaginal tissue in the post-menopausal women with and without Pelvic organ prolapse (POP) suggests that prolonged hypoxia probably has an important role in the aetiopathogenesis of POP PMID: 29578197
  57. Daxx directly binds to the DNA-binding domain of Slug, impeding histone deacetylase 1 (HDAC1) recruitment and antagonizing Slug E-box binding. This, in turn, stimulates E-cadherin and occludin expression and suppresses Slug-mediated epithelial-mesenchymal transition (EMT) and cell invasiveness. Daxx can act as a repressor in controlling HIF-1alpha/HDAC1/Slug-mediated cancer cell invasion. PMID: 28004751
  58. The HIF1alphainduced expression of Runx2 and ALP may be completely dependent on the expression levels of Foxo1, and in turn, osteocalcin may be partially dependent on Foxo1 expression. PMID: 29512721
  59. Importantly, high expression levels of HIF-1alpha/TGF-beta2/GLI2 correlated robustly with the patient relapse following chemotherapy, highlighting a potential biomarker and therapeutic target for chemoresistance in colorectal cancer. PMID: 29891662
  60. Reactive oxygen species-mediated EGFR/MEK/ERK/HIF-1A loop regulates glucose metabolism in pancreatic cancer. PMID: 29702094
  61. Review of the role of HIF1A in skin aging and rejuvenation. PMID: 29596193
  62. High HIF1A expression is associated with osteosarcoma. PMID: 29328452
  63. Our data suggest that HIF-1alpha plays a critical role in the remodeling of the extracellular matrix as well as metastasizing process of follicular thyroid carcinoma PMID: 28474313
  64. Down-regulation of miR-153 may contribute to enhanced expression of HIF-1alpha in the mesial temporal lobe epilepsy patients.MiR-153 directly targets HIF-1alpha 3'UTR. PMID: 27554040
  65. we found that knockdown of FNDC3B could suppress the migratory and invasive abilities of OTSCC cells. In addition, treating OTSCC cells with CoCl2 (a hypoxia mimetic agent) upregulated the mRNA and protein expression of FNDC3B via HIF-1alpha PMID: 29393475
  66. Genetic variation at the 8q24.21 renal cancer susceptibility locus affects HIF1A and HIF1B binding to a MYC enhancer. PMID: 27774982
  67. MiR-199a may inhibit proliferation and induce apoptosis in HAs cells via targeting HIF1A. PMID: 29268640
  68. ILKAP physically interacted with HIF-1A and induced its dephosphorylation. Both the HIF-1A-p53 interaction and apoptosis relied on ILKAP. PMID: 29742494
  69. Hypoxia promotes retinoblastoma cell line HXO-RB44 invasion by activating HIF-1alpha/MMP9 signaling pathway. PMID: 29243776
  70. This study demonstrates increased placental expression of HIF-1alpha and CHOP in preeclampsia compared to normotensive pregnancies which correlate to their increased syncytiotrophoblast microvesicles concentration in maternal circulation. PMID: 29127866
  71. Data do not support a role for the NFKB1 and HIF1A polymorphisms in the pathogenesis of bowel disease. PMID: 29307990
  72. Results suggest an interplay of the production and action of hydrogen sulfide during hypoxia with subsequent erythropoietin production regulated by HIF-1alpha and HIF-2alpha. PMID: 26880412
  73. that the reduced expression of LimD1 and VHL might have synergistic effect on induction of HIF1alpha resulting increased cellular proliferation and progression of the disease. PMID: 29033184
  74. All these data suggest the possibility that hypoxiamediated PrPC serves an important role in angiogenesis. Therefore, the present review summarizes the characteristics of PrPC, which is produced by HIF1alpha in hypoxia, as it relates to angiogenesis PMID: 28901450
  75. LncRNA CPS1-IT1 suppresses EMT and metastasis of colorectal cancer by inhibiting hypoxia-induced autophagy through inactivation of HIF-1alpha PMID: 29017924
  76. both the value and the level of Ki-67 expression were correlated positively with the normalized iodine concentration (NIC) values (r=0.344, P=0.002 and r=0.248, P=0.026); HIF-1alpha expression was correlated positively with the NIC values of the RC (r=0.598, P<0.001) PMID: 29103468
  77. Our data showed that ampelopsin inhibited angiogenesis with no cytotoxicity by suppressing both VEGFR2 signaling and HIF-1alpha expression. These results suggest that Hovenia dulcis Thunb. and its active compound ampelopsin exhibit potent antiangiogenic activities and therefore could be valuable for the prevention and treatment of angiogenesis-related diseases including cancer. PMID: 29039561
  78. the findings of the present study demonstrated that MIF is a key component in lung cancer progression through promoting the Warburg effect, and that the novel MIF/NFkappaB/HIF1alpha axis may prove to be useful for the development of new strategies for treating patients with lung cancer PMID: 29207023
  79. the present study demonstrated that sevoflurane decreased the malignant behavior of head and neck squamous cell carcinoma cell lines in vitro, which was associated with activation of the HIF-1alpha pathway PMID: 29207062
  80. Hypoxia-inducible factor-1alpha was found to be an important effector element in the localized control of human cutaneous and mucocutaneous lesions. PMID: 29717578
  81. The cell surface PDGFRB is a major link between high glucose and its effectors Hif1a and TGFB for induction of diabetic mesangial cell hypertrophy. PMID: 28951244
  82. study suggests that high tumor tissue ascorbate level could limit the expression of HIF-1alpha and its targets in thyroid lesions. PMID: 29084538
  83. HIF1alpha may be involved in the molecular mechanisms of endometrial dysfunction in women with PCOS, particularly in those who are overweight. PMID: 29115598
  84. Exogenous proline stimulates type I collagen and HIF-1alpha expression and the process is attenuated by glutamine in human skin fibroblasts. PMID: 28526934
  85. The results showed that IL-6 treatment could induce upregulation of HIF-1alpha via the activation of STAT3 in MDA-MB-231 cells, which consequently contributed to its effect against chemotherapeutic drug-induced cytotoxicity and cell apoptosis. PMID: 29341321
  86. STAT3 regulates angiogenic and metabolic pathways before HIF1alpha, suggesting that HIF1alpha is not the initiating trans-acting factor in the response of pericytes to ischemia. PMID: 29518129
  87. These findings suggest that using Hph-1-G4D to overexpress HIF1A might be useful for transferring genes and regenerating tissues. PMID: 28660411
  88. Study demonstrated that hypoxia inhibited human endothelial colony-forming cells (ECFCs) functions in a time- and O2 level-dependently manner, including cell proliferation, migration, and angiogenesis. HIF-1alpha may be the determinant of hypoxia-induced ECFCs dysfunction. PMID: 28964937
  89. associate dysfunction in Fe-S cluster biogenesis with cleavage of VDAC1 PMID: 29596470
  90. Multivariate analyses showed that higher HIF-1alpha expression, but not high VEGF, were associated significantly and independently with increased tumor aggressiveness as derived from several well-established aggressiveness criteria. PMID: 28885396
  91. we first report that PVT1 promotes expression of HIF-1alpha, a critical endogenous hypoxia marker, in NSCLC. With this finding, we demonstrated that PVT1 modulated HIF-1alpha by competing for miR-199a-5p as a ceRNA to regulate cell proliferation. PMID: 29115513
  92. AA can protect cardiomyocytes against hypoxia-induced apoptosis through regulating the miR-1290/HIF3A/HIF-1alpha axis, and miR-1290 may be a potential target in the prevention of myocardial ischemia-reperfusion injury PMID: 28686797
  93. this paper shows that IL-1beta upregulates Muc5ac expression via NF-kappaB-induced HIF-1alpha in asthma PMID: 29031476
  94. The role of HIF-1alpha in angiogenesis and osteogenesis PMID: 29111380
  95. Decreased HIF-1alpha may contribute to impaired megakaryopoiesis in immune thrombocytopenia. PMID: 28771276
  96. HIF-2 expression has complicated roles in different cancer types PMID: 28690120
  97. MCF-7 cell proliferation was increased, invasion and migration were enhanced, with upregulated expression of HIF-1alpha mRNA and miR-210 in the hypoxic and anaerobic group following time extension. PMID: 29053417
  98. HIF-1alpha, which suppressed miR-340-5p promoter activation through HRE motifs, was induced by FA. The induction of b-catenin signaling by FA was consistent with an enhancement in osteogenesis of FA-treated MSC, which could be attenuated by miR-340-5p overexpression. Analysis of the signaling networks induced by FA reveals that hypoxia may promote the osteogenic program in mesenchymal stem cells via a novel microRNA pa... PMID: 28764862
  99. We studied the hypoxic activation of the transcription factors HIF-1alpha and HIF-2alpha in endothelial cells within a spatial linear gradient of oxygen. Quantification of the nuclear to cytosolic ratio of HIF immunofluorescent staining demonstrated that the threshold for HIF-1alpha activation was below 2.5% O2 while HIF-2alpha was activated throughout the entire linear gradient. PMID: 28840922
  100. Study confirmed that hypoxia increased MTDH expression via HIF-1alpha expression. Knockdown of MTDH expression in head and neck squamous cell carcinoma (HNSCC) cell lines interrupted hypoxiainduced metastasis and glycolysis. Furthermore, reduced MTDH expression decreased HIF-1alpha expression. These results indicated the presence of a positive feedback loop between MTDH and HIF-1alpha in HNSCC. PMID: 28901527

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Subcellular Location Cytoplasm, Nucleus, Nucleus speckle
Tissue Specificity Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumo
Database Links

HGNC: 4910

OMIM: 603348

KEGG: hsa:3091

STRING: 9606.ENSP00000338018

UniGene: Hs.597216

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