HIF1A, hypoxia-inducible factor 1-alpha, is a transcription factor that is activated under oxygen-deprived conditions in human and mammalian cells. It governs a range of gene expressions, impacting cellular metabolism, proliferation, and survival, thus playing a critical role in cellular adaptation to hypoxia and fostering angiogenesis. HIF1A is vital for embryonic development, tissue homeostasis, and the response to hypoxic stress. Its dysregulation is implicated in pathological processes such as tumorigenesis, inflammation, and immune disorders, making it a significant factor in both physiological and pathological process.
HIF1A Recombinant Monoclonal Antibody (CSB-RA624113MA1HU)
Validated Data
Immunofluorescence staining of Hela cell with CSB-RA624113MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Hela cells stained with CSB-RA624113MA1HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was rabbit IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,001 events was performed.
Human hypoxia-inducible factor 1α(HIF-1α) ELISA kit (CSB-E12112h)
Validated Data
Code: CSB-E12112h
Size: 96T,5×96T,10×96T
Sensitivity: 15.6 pg/mL
Detection Range: 62.5 pg/mL-4000 pg/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The following HIF1A reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
HIF1A Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA010351GA01HU | HIF1A Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA12807A0Rb | HIF1A Antibody | Human | ELISA, IHC, IF |
CSB-PA12807B0Rb | HIF1A Antibody, HRP conjugated | Human | ELISA |
CSB-PA12807C0Rb | HIF1A Antibody, FITC conjugated | Human | |
CSB-PA12807D0Rb | HIF1A Antibody, Biotin conjugated | Human | ELISA |
CSB-PA930291 | HIF1A Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA952643 | HIF1A Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA000432 | HIF1A Antibody | Mouse,Rat | WB |
CSB-PA002906 | HIF1A Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA077072 | HIF1A Antibody | Human | ELISA,IHC |
CSB-PA968396 | HIF1A Antibody | Human | ELISA,WB,IHC |
CSB-PA928186 | HIF1A Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA578243 | HIF1A Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-RA263943A0HU | HIF1A Recombinant Monoclonal Antibody | Human | ELISA, IF |
CSB-RA624113MA1HU | HIF1A Recombinant Monoclonal Antibody | Human | ELISA, IF, FC |
HIF1A Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP010351RA CSB-EP010351RA CSB-BP010351RA CSB-MP010351RA CSB-EP010351RA-B |
Recombinant Rat Hypoxia-inducible factor 1-alpha (Hif1a), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HIF1A Proteins for Bos mutus grunniens (Wild yak) (Bos grunniens)
Code | Product Name | Source |
---|---|---|
CSB-YP611302BPD CSB-EP611302BPD CSB-BP611302BPD CSB-MP611302BPD CSB-EP611302BPD-B |
Recombinant Bos mutus grunniens Hypoxia-inducible factor 1-alpha (HIF1A), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HIF1A Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP624113HU CSB-EP624113HU CSB-BP624113HU CSB-MP624113HU CSB-EP624113HU-B |
Recombinant Human Hypoxia-inducible factor 1-alpha (HIF1A), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP624113HU | Recombinant Human Hypoxia-inducible factor 1-alpha (HIF1A), partial | E.coli |
HIF1A Proteins for Eospalax fontanierii baileyi (Plateau zokor) (Eospalax baileyi)
Code | Product Name | Source |
---|---|---|
CSB-YP653038EAAY CSB-EP653038EAAY CSB-BP653038EAAY CSB-MP653038EAAY CSB-EP653038EAAY-B |
Recombinant Eospalax baileyi Hypoxia-inducible factor 1-alpha (HIF1A), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HIF1A Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-EP730731MO CSB-BP730731MO CSB-MP730731MO CSB-EP730731MO-B |
Recombinant Mouse Hypoxia-inducible factor 1-alpha (Hif1a), partial | E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HIF1A Proteins for Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Code | Product Name | Source |
---|---|---|
CSB-YP858481OEI CSB-EP858481OEI CSB-BP858481OEI CSB-MP858481OEI CSB-EP858481OEI-B |
Recombinant Oncorhynchus mykiss Hypoxia-inducible factor 1-alpha (hif1a), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HIF1A Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP867421XBE CSB-EP867421XBE CSB-BP867421XBE CSB-MP867421XBE CSB-EP867421XBE-B |
Recombinant Xenopus laevis Hypoxia-inducible factor 1-alpha (hif1a), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HIF1A Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP895019BO CSB-EP895019BO CSB-BP895019BO CSB-MP895019BO CSB-EP895019BO-B |
Recombinant Bovine Hypoxia-inducible factor 1-alpha (HIF1A), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HIF1A Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP896655CH CSB-EP896655CH CSB-BP896655CH CSB-MP896655CH CSB-EP896655CH-B |
Recombinant Chicken Hypoxia-inducible factor 1-alpha (HIF1A), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HIF1A ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E12112h | Human hypoxia-inducible factor 1α(HIF-1α) ELISA kit | serum, plasma, tissue homogenates | 15.6 pg/mL |
HIF1A ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E08541m | Mouse hypoxia-inducible factor 1α,HIF-1α ELISA Kit | serum, plasma | 0.156 pg/mL |
HIF1A ELISA Kit for Rattus norvegicus (Rat)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E08540r | Rat hypoxia-inducible factor 1α,HIF-1α ELISA Kit | serum, plasma, tissue homogenates | 0.78 pg/mL |