Product Details

Alternative Names

ICAM3; Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD antigen CD50

Abbreviation

ICAM3

Uniprot No.

P32942

Species

Homo sapiens (Human)

Sample Types

serum, plasma,tissue homogenates

Detection Range

31.25 pg/ml - 2000 pg/ml

Sensitivity

7.8 pg/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	225.927	236.470	246.378	218.347	231.580	242.642
SD	0.036	0.044	0.031	0.036	0.046	0.029
CV(%)	4.250	5.052	3.865	4.311	5.336	3.532

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ICAM-3/CD50 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

89-100

1:2

Average %

Range %

84-92

1:4

Average %

103

Range %

98-107

1:8

Average %

Range %

89-97

Recovery

The recovery of human ICAM-3/CD50 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	96	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.752	2.654	2.703	2.548
1000	1.981	2.015	1.998	1.843
500	1.407	1.386	1.397	1.242
250	0.846	0.872	0.859	0.704
125	0.529	0.508	0.519	0.364
62.5	0.318	0.321	0.320	0.165
31.25	0.235	0.229	0.232	0.077
0	0.153	0.157	0.155

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human ICAM3 ELISA Kit was designed for the quantitative measurement of Human ICAM3 protein in serum, plasma,tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/ml - 2000 pg/ml and the sensitivity is 7.8 pg/ml.

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Target Background

Function
(From Uniprot)

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages.

Gene References into Functions

exploration of the underlying mechanism demonstrated that ICAM3 not only binds to LFA-1 with its extracellular domain and structure protein ERM but also to lamellipodia with its intracellular domain which causes a tension that pulls cells apart (metastasis). PMID: 29729315
Increased expression of PECAM-1, ICAM-3, and VCAM-1 in colonic biopsies from patients with inflammatory bowel disease (IBD) in clinical remission is associated with subsequent flares; this suggests that increases in the expression of these proteins may be early events that lead to flares in patients with IBD PMID: 27552332
ICAM3 acts as recognition receptors in the phagocytosis portals of macrophages for engulfment of apoptotic neutrophils. PMID: 23775590
this data clearly indicate that ICAM-3 promotes drug resistance via inhibition of apoptosis. PMID: 24177012
Intercellular adhesion molecule (ICAM)-3 mRNA is upregulated in non-adherent endothelial forming cells. PMID: 23144795
Results indicate that the ICAM-3 gene promoter is negatively regulated by RUNX3. PMID: 22479382
analysis of activated apoptotic cells induce dendritic cell maturation via engagement of Toll-like receptor 4 (TLR4), dendritic cell-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing nonintegrin (DC-SIGN), and beta2 integrins PMID: 22396536
ICAM-3 may be an important adhesion molecule involved in chemotaxis to apoptotic human leukocytes. PMID: 22117198
the molecular basis of allergen-induced Th2 cell polarization PMID: 22205703
the cross-talk between neutrophils and NK cells is mediated by ICAM-3 and CD11d/CD18, respectively. PMID: 21712539
Single nucleotide polymorphisms in ICAM3 gene is associated with lymphoma. PMID: 21239057
induction of morphological polarization in primary T lymphocytes and Jurkat cells enhances Kidins220/ARMS colocalization with ICAM-3 PMID: 21381019
CCR1 antagonist, BX471, did not significantly alter ICAM-3 expression in relapsing-remitting multiple sclerosis patients. PMID: 20086017
ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB PMID: 19956847
no significant risk association was found for SARS infection for the ICAM-3 Asp143Gly SNP. PMID: 19801714
A novel serine-rich motif in the intercellular adhesion molecule 3 is critical for its ezrin/radixin/moesin-directed subcellular targeting PMID: 11784723
identification of DC-SIGN binding sites PMID: 11799126
interactions with DC-SIGN does not promote DC-SIGN mediated HIV-1 transmission PMID: 12021323
Expression of DC-SIGN and its ligand, ICAM-3, is found in substantial amounts only in RA synovium, suggesting that their interaction is implicated in the additional activation of synovial macrophages that leads to the production of EMMPRIN and MMP-1. PMID: 12571844
ICAM-3 is highly expressed on the surface of human eosinophils and has a role in the downregulation of GM-CSF production. PMID: 12743567
Relationship of intercellular adhesion molecule-3 and hepatocyte growth factor with amyloidosis A in chronic renal-failure patients. There was a higher density of intercellular adhesion molecule-3-positive cells in the patients with amyloidosis A. PMID: 14704632
ICAM-3 is expressed on human bone marrow endothelial cells and controls endothelial integrity via reactive oxygen species-dependent signaling. PMID: 14726630
acts as a costimulating molecule to increase HIV-1 transcription and viral replication, a process allowing productive infection of quiescent CD4+ T lymphocytes. PMID: 15163761
Expression of ICAM-3 can be used as a valuable biomarker to predict the radiation resistance in cervical cancer that occurs during radiotherapy. PMID: 15880373
The results suggest that ICAM-3 assists in the interaction of granulocytes with DC-SIGN of dendritic cells. PMID: 17145745
Patients with SARS homozygous for ICAM3 Gly143 showed significant association with higher lactate dehydrogenase levels and lower total white blood cell counts. PMID: 17570115
This is the first case of CD20 positive mycosis fungoides involving a lymph node to be reported in the literature. PMID: 18261116
extended alpha(L)beta(2) with an open headpiece is required for ICAM-3 adhesion PMID: 18354203

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Immunoglobulin superfamily, ICAM family

Tissue Specificity

Leukocytes.

Database Links

HGNC: 5346

OMIM: 146631

KEGG: hsa:3385

STRING: 9606.ENSP00000160262

UniGene: Hs.654563

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Code	CSB-E07949h
Size	96T,5×96T,10×96T
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Human intercellular adhesion molecule 3,ICAM-3 ELISA Kit

Product Details

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Target Background