Code | CSB-E13348h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human LRP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 93 | ||||
Range % | 88-97 | |||||
1:2 | Average % | 84 | ||||
Range % | 80-89 | |||||
1:4 | Average % | 102 | ||||
Range % | 96-108 | |||||
1:8 | Average % | 97 | ||||
Range % | 91-102 |
The recovery of human LRP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 86 | 82-94 | ||||
EDTA plasma (n=4) | 99 | 92-107 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human LRP1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human LRP1 in multiple samples, including serum, plasma, cell lysates, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the LRP1 levels in the sample and is measured at 450 nm through a microplate reader.
LRP1 is involved in two major physiological processes: endocytosis and regulation of signaling pathways, which play an important role in multiple biological activities, including regulation of cell growth and differentiation, modulation of cytoskeletal organization angiogenesis, cell migration, and regulation of protease degradation for tissue invasion. LRP1 mediates the endocytosis of a diverse set of extracellular ligands that play important roles in tumor progression. Additionally, LRP1 can initiate and regulate diverse signaling pathways, including MAPK, insulin receptor (IR), serine/AKT, ERK, and JNK pathways. LRP1 has been postulated to participate in numerous diverse physiological and pathological processes ranging from plasma lipoprotein homeostasis, atherosclerosis, tumor evolution, and fibrinolysis to neuronal regeneration and survival.
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