Product Details

Target Name

melanin-concentrating hormone (PMCH)

Alternative Names

PMCH ELISA Kit; MCHPro-MCH [Cleaved into: Neuropeptide-glycine-glutamic acid ELISA Kit; NGE ELISA Kit; Neuropeptide G-E); Neuropeptide-glutamic acid-isoleucine ELISA Kit; NEI ELISA Kit; Neuropeptide E-I); Melanin-concentrating hormone ELISA Kit; MCH)] ELISA Kit

Abbreviation

PMCH

Uniprot No.

P20382

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PMCH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	87
	Range %	83-91
1:4	Average %	92
	Range %	88-96
1:8	Average %	90
	Range %	85-94

Recovery

The recovery of human PMCH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-104
EDTA plasma (n=4)	84	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.682	2.533	2.608	2.400
500	2.040	1.946	1.993	1.785
250	1.419	1.357	1.388	1.180
125	0.927	0.975	0.951	0.743
62.5	0.654	0.689	0.672	0.464
31.2	0.408	0.392	0.400	0.192
15.6	0.335	0.324	0.330	0.122
0	0.204	0.211	0.208

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human PMCH ELISA Kit was designed for the quantitative measurement of Human PMCH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

■ Q&A

Do you have a reference range for human plasma from EDTA blood? What are the antibodies targeted? I am mostly interested in terminal 19 residues.

Generally, we test normal serum and plasma samples without obvious pathology, please consult us for specific sample testing.

Target Background

Function
(From Uniprot)

MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.

Gene References into Functions

MCH is an anabolic neuropeptide with multiple and diverse physiological functions including a key role in energy homoeostasis; evidence suggests that, in the reproductive context, MCH is a modulator rather than a primary regulator. PMID: 23884861
Cerebrospinal fluid (CSF) levels were measured in 33 patients with Alzheimer's disease (AD) and 33 healthy subjects, to investigate the role of MCH and HCRT-1 in AD. PMID: 23667582
the changes in the levels of two hypothalamic neuropeptides, hypocretin-1 and melanin-concentrating hormone, measured in the human amygdala, are reported. PMID: 23462990
Melanin-concentrating hormone (MCH) neurons of human hypothalamus, as well as the MCH neurons of mouse hypothalamus, contain IL-6Ralpha. PMID: 22295972
PMCH mRNA expression level was increased 1.85 times (P < 0.01) in vitiligo uninvolved skin and 5.36 times (P < 0.01) in involved skin compared to healthy control skin. PMID: 20888736
Melanin-concentrating hormone is expressed and functional in human skin PMID: 12176038
RT-PCR analysis revealed a low expression of prepro-MCH and MCH receptor 1 (MCHR1) but not of MCHR2 transcript in tissular and peripheral blood immune cells PMID: 12220661
Review. Melanin-concentrating hormone (MCH) is a cyclic neuropeptide, which centrally regulates food intake and stress. MCH-R1 antagonists can probe additional functions of the peptide, both in the brain and in the peripheral nervous system. PMID: 12885269
Serum levels could serve as useful peripheral markers of changes in energy homeostasis. PMID: 15546902
hMCH increased water intake independent of food intake. It significantly increased both sucrose solution and food intake. PMID: 15870910
Single nucleotide polymorphisms in pro-melanin-concentrating hormone may be associated with obesity and a greater body mass index in olanzapine-treated schizophrenic patients PMID: 17541984
expression of PMCH by activated Th2 cells in vivo may directly link allergic inflammation to energy homeostasis and may contribute to the association between asthma and obesity PMID: 17640905
The total number of hypocretin-1 neurons was significantly reduced by 30% in HD brains (P = 0.015), while the total number of MCH neurons was not significantly altered (P = 0.100). PMID: 18498421
MCH has a role in inflammatory processes in the intestine PMID: 18650383

Hide All

Subcellular Location

Secreted.

Protein Families

Melanin-concentrating hormone family

Tissue Specificity

Predominantly expressed in lateral hypothalamus, also detected in pallidum, neocortex and cerebellum. Also found in thymus, brown adipose tissue, duodenum and testis (spermatogonia, early spermatocytes and Sertoli cells). No expression in peripheral blood

Database Links

HGNC: 9109

OMIM: 176795

KEGG: hsa:5367

STRING: 9606.ENSP00000332225

UniGene: Hs.707990

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-EL018230HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human melanin-concentrating hormone (PMCH) ELISA kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background