Human phosphoglycerate mutase 2,PGAM2 ELISA Kit

Code CSB-E11282h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human PGAM2 ELISA Kit was designed for the quantitative measurement of Human PGAM2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Name phosphoglycerate mutase 2 (muscle)
Alternative Names BPG dependent PGAM 2 ELISA Kit; BPG-dependent PGAM 2 ELISA Kit; GSD10 ELISA Kit; MGC88743 ELISA Kit; Muscle specific phosphoglycerate mutase ELISA Kit; Muscle-specific phosphoglycerate mutase ELISA Kit; OTTHUMP00000207787 ELISA Kit; PGAM 2 ELISA Kit; PGAM M ELISA Kit; PGAM-M ELISA Kit; Pgam2 ELISA Kit; PGAM2_HUMAN ELISA Kit; PGAMM ELISA Kit; phosphoglycerate mutase 2 (muscle) ELISA Kit; Phosphoglycerate mutase 2 ELISA Kit; Phosphoglycerate mutase isozyme M ELISA Kit
Abbreviation PGAM2
Uniprot No. P15259
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PGAM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 82-94  
1:2 Average % 102  
Range % 98-105  
1:4 Average % 95  
Range % 89-99  
1:8 Average % 98  
Range % 91-102  
Recovery
The recovery of human PGAM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 98 92-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.526 2.417 2.472 2.327  
500 1.608 1.505 1.557 1.412  
250 0.884 0.855 0.870 0.725  
125 0.486 0.494 0.490 0.345  
62.5 0.329 0.306 0.318 0.173  
31.2 0.265 0.257 0.261 0.116  
15.6 0.197 0.189 0.193 0.048  
0 0.145 0.144 0.145    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.
Gene References into Functions
  1. These results reveal a mechanism of PGAM2 regulation and NADPH homeostasis in response to oxidative stress that impacts cell proliferation and tumor growth. PMID: 24786789
Involvement in disease Glycogen storage disease 10 (GSD10)
Protein Families Phosphoglycerate mutase family, BPG-dependent PGAM subfamily
Tissue Specificity Expressed in the heart and muscle. Not found in the liver and brain.
Database Links

HGNC: 8889

OMIM: 261670

KEGG: hsa:5224

STRING: 9606.ENSP00000297283

UniGene: Hs.632642

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