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Human pro-prostate specific antigen (Pro-PSA) ELISA kit

Citations (1) Protocol MSDS
Code CSB-ET027786HU
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
pro-prostate specific antigen (Pro-PSA)
Abbreviation
Pro-PSA
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
125 pg/mL-4000 pg/mL
Sensitivity
62.5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<20%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-PSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 89-99  
1:2 Average % 90  
Range % 84-94  
1:4 Average % 105  
Range % 98-108  
1:8 Average % 92  
Range % 88-96  
Recovery
The recovery of human Pro-PSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 83-94  
EDTA plasma (n=4) 93 89-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
4000 2.351 2.201 2.276    
2000 1.412 1.356 1.384    
1000 0.848 0.826 0.837    
250 0.234 0.247 0.241    
125 0.105 0.109 0.107    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human Pro-PSA ELISA Kit was designed for the quantitative measurement of Human Pro-PSA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-4000 pg/mL and the sensitivity is 62.5 pg/mL.

Citations

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