Code | CSB-E13058h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human PR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 90 | ||||
Range % | 86-95 | |||||
1:2 | Average % | 88 | ||||
Range % | 81-93 | |||||
1:4 | Average % | 98 | ||||
Range % | 93-102 | |||||
1:8 | Average % | 102 | ||||
Range % | 98-106 |
The recovery of human PR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 104 | 94-110 | ||||
EDTA plasma (n=4) | 92 | 88-97 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
CUSABIO's human proteinase 3 (PR3) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PR3 in serum, tissue homogenates, plasma, or urine. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of PR3 in samples. The color intensity is measured at 450 nm via a microplate reader.
PR3 (PRTN3) is an enzyme released during neutrophilic inflammation and is capable of cleaving many targets including key structural proteins of the lung. In addition to cleaving structural proteins resulting in tissue remodeling, PR3 also assists in the defensive immune role of the neutrophil including modulating various cellular activities, cleaving host protein into antibacterial peptides, and activating pro-inflammatory cytokines. PR3 also regulates a variety of cytokine functions, which impact processes such as metabolism and inflammasome generation. It also exerts a pro-inflammatory role by interacting with the complement pathway.
There are currently no reviews for this product.