Human prothrombin fragment 1+2,F1+2 ELISA kit

Instructions
Code CSB-E09822h
Size 96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name prothrombin fragment 1+2,F1+2
Abbreviation F1+2
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.03 nmol/L -2 nmol/L
Sensitivity 0.007 nmol/L
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 89-99  
1:2 Average % 98  
Range % 93-102  
1:4 Average % 86  
Range % 81-93  
1:8 Average % 92  
Range % 88-97  
Recovery
The recovery of human F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-96  
EDTA plasma (n=4) 87 83-90  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nmol/ml. OD1 OD2 Average Corrected  
2 2.296 2.274 2.285 2.137  
1 1.445 1.498 1.472 1.324  
0.5 0.916 0.936 0.926 0.778  
0.25 0.538 0.568 0.553 0.405  
0.125 0.376 0.398 0.387 0.239  
0.06 0.270 0.291 0.281 0.133  
0.03 0.218 0.216 0.217 0.069  
0 0.146 0.149 0.148    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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