Target Name
prothrombin fragment 1+2,F1+2
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.03 nmol/L -2 nmol/L
Detection Wavelength
450 nm
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
89-99 |
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| 1:2 |
Average % |
98 |
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| Range % |
93-102 |
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| 1:4 |
Average % |
86 |
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| Range % |
81-93 |
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| 1:8 |
Average % |
92 |
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| Range % |
88-97 |
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Recovery
| The recovery of human F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
90-96 |
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| EDTA plasma (n=4) |
87 |
83-90 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| nmol/ml. |
OD1 |
OD2 |
Average |
Corrected |
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| 2 |
2.296 |
2.274 |
2.285 |
2.137 |
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| 1 |
1.445 |
1.498 |
1.472 |
1.324 |
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| 0.5 |
0.916 |
0.936 |
0.926 |
0.778 |
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| 0.25 |
0.538 |
0.568 |
0.553 |
0.405 |
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| 0.125 |
0.376 |
0.398 |
0.387 |
0.239 |
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| 0.06 |
0.270 |
0.291 |
0.281 |
0.133 |
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| 0.03 |
0.218 |
0.216 |
0.217 |
0.069 |
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| 0 |
0.146 |
0.149 |
0.148 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
