Human pyridinoline (PYD) ELISA Kit

Code CSB-E09702h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
Pyridinoline crosslinks,PYD
Alternative Names
Homo sapiens (Human)
Sample Types
serum, plasma, urine
Detection Range
12.5 ng/mL-800 ng/mL
3.12 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human PYD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 88-100  
1:2 Average % 92  
Range % 86-99  
1:4 Average % 87  
Range % 80-94  
1:8 Average % 97  
Range % 92-102  
The recovery of human PYD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 85-99  
EDTA plasma (n=4) 98 92-104  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
800 2.501 2.450 2.476 2.343  
400 1.934 2.009 1.972 1.839  
200 1.295 1.301 1.298 1.165  
100 0.718 0.775 0.747 0.614  
50 0.483 0.495 0.489 0.356  
25 0.331 0.351 0.341 0.208  
12.5 0.226 0.235 0.231 0.098  
0 0.136 0.129 0.133    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Human PYD ELISA Kit was designed for the quantitative measurement of Human PYD protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

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