Human pyridinoline (PYD) ELISA Kit

Instructions
Code CSB-E09702h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Pyridinoline crosslinks,PYD
Alternative Names N/A
Abbreviation PYD
Species Homo sapiens (Human)
Sample Types serum, plasma, urine
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PYD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 88-100  
1:2 Average % 92  
Range % 86-99  
1:4 Average % 87  
Range % 80-94  
1:8 Average % 97  
Range % 92-102  
Recovery
The recovery of human PYD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 85-99  
EDTA plasma (n=4) 98 92-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
800 2.501 2.450 2.476 2.343  
400 1.934 2.009 1.972 1.839  
200 1.295 1.301 1.298 1.165  
100 0.718 0.775 0.747 0.614  
50 0.483 0.495 0.489 0.356  
25 0.331 0.351 0.341 0.208  
12.5 0.226 0.235 0.231 0.098  
0 0.136 0.129 0.133    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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