Human regenerating gene Ⅳ,REG-4 ELISA Kit

Code CSB-E11890h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9BYZ8
Lead Time 3-5 working days
Abbreviation REG4
Target Name regenerating islet-derived family, member 4
Alias GISP, REG-IV, RELP, OTTHUMP00000014031|gastrointestinal secretory protein|regenerating gene type IV
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 22977
RGD 1303341
MGI 1914959
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human REG-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 83-92  
1:2 Average % 92  
Range % 90-95  
1:4 Average % 96  
Range % 93-99  
1:8 Average % 95  
Range % 88-98  
Recovery
The recovery of human REG-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 89-94  
EDTA plasma (n=4) 92 90-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.832 2.801 2.817 2.676  
10 2.238 2.187 2.213 2.072  
5 1.419 1.385 1.402 1.261  
2.5 0.673 0.654 0.664 0.523  
1.25 0.434 0.411 0.423 0.282  
0.625 0.281 0.269 0.275 0.134  
0.312 0.211 0.202 0.207 0.066  
0 0.143 0.139 0.141    
References
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Function Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.
Subcellular Location Secreted
Tissue Specificity Highly expressed in the gastrointestinal tract including the duodenum, jejunum, ileum, ileocecum, appendix, descending colon, pancreas and small intestine. Weakly expressed in normal colon and stomach. Strongly expressed in most colorectal tumors than in
Database Links

HGNC: 22977

OMIM: 609846

KEGG: hsa:83998

STRING: 9606.ENSP00000256585

UniGene: Hs.660883

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