Human soluble amyloid precursor protein α (sAPPα) ELISA kit

Code CSB-EQ027464HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details


This Human sAPPα ELISA Kit was designed for the quantitative measurement of Human sAPPα protein in serum, plasma, tissue homogenates, cell culture supernates, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

Target Name soluble amyloid precursor protein α (sAPPα)
Abbreviation sAPPα
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell culture supernates, cerebrospinal fluid (CSF)
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human sAPPα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 82-94  
1:2 Average % 101  
Range % 90-105  
1:4 Average % 91  
Range % 85-94  
1:8 Average % 93  
Range % 85-97  
The recovery of human sAPPα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 85-97  
EDTA plasma (n=4) 97 90-101  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
800 1.822 1.902 1.862 1.729  
400 1.454 1.351 1.403 1.270  
200 0.859 0.901 0.880 0.747  
100 0.537 0.548 0.543 0.410  
50 0.339 0.345 0.342 0.209  
25 0.234 0.222 0.228 0.095  
12.5 0.166 0.172 0.169 0.036  
0 0.132 0.134 0.133    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
7505 Fannin St Ste 610-322 Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2022 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1