Human type Ⅱ collagen helical peptide,HELIX-Ⅱ ELISA Kit

Code CSB-E09708h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
type Ⅱ collagen helical peptide,HELIX-Ⅱ
Alternative Names
type 2 collagen helical peptide,HELIX-2,type Ⅱ collagen helical peptide,HELIX-Ⅱ
Abbreviation
HELIXⅡ
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine
Detection Range
1.25 ng/mL-80 ng/mL
Sensitivity
0.312 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HELIX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-99  
1:2 Average % 89  
Range % 83-94  
1:4 Average % 99  
Range % 94-103  
1:8 Average % 104  
Range % 100-112  
Recovery
The recovery of human HELIX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 84 80-88  
EDTA plasma (n=4) 91 86-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
80 2.178 2.114 2.146 2.043  
40 1.674 1.581 1.628 1.525  
20 1.116 1.061 1.089 0.986  
10 0.626 0.603 0.615 0.512  
5 0.423 0.407 0.415 0.312  
2.5 0.252 0.239 0.246 0.143  
1.25 0.193 0.184 0.189 0.086  
0 0.104 0.101 0.103    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human HELIXⅡ ELISA Kit was designed for the quantitative measurement of Human HELIXⅡ protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.312 ng/mL .

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