Human vitamin K-dependent protein Z (PROZ) ELISA kit_x000D_

Instructions
Code CSB-E09904h
Size 96T,5×96T,10×96T

Product Details

Target Name vitamin K-dependent protein Z
Alternative Names PZ,protein Z, vitamin K-dependent plasma glycoprotein
Abbreviation PROZ
Uniprot No. P22891
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 ng/mL-8000 ng/mL
Sensitivity 31.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PROZ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 89-107  
1:2 Average % 90  
Range % 87-103  
1:4 Average % 89  
Range % 82-98  
1:8 Average % 93  
Range % 87-105  
Recovery
The recovery of human PROZ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 86-104  
EDTA plasma (n=4) 95 87-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
8000 2.601 2.525 2.563 2.425  
4000 1.882 1.910 1.896 1.758  
2000 1.175 1.183 1.179 1.041  
1000 0.658 0.682 0.670 0.532  
500 0.364 0.371 0.368 0.230  
250 0.249 0.260 0.255 0.117  
125 0.208 0.211 0.210 0.072  
0 0.139 0.137 0.138    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. Inhibits activity of the coagulation protease factor Xa in the presence of SERPINA10, calcium and phospholipids.
Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Plasma.
Database Links

HGNC: 9460

OMIM: 176895

KEGG: hsa:8858

STRING: 9606.ENSP00000364697

UniGene: Hs.1011

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