Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5) ELISA kit

Instructions
Code CSB-EL001312MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ADAM metallopeptidase with thrombospondin type 1 motif, 5
Alternative Names Adamts5 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs 5 ELISA Kit; ADAM-TS 5 ELISA Kit; ADAM-TS5 ELISA Kit; ADAMTS-5 ELISA Kit; EC 3.4.24.- ELISA Kit; ADMP-2 ELISA Kit; Aggrecanase-2 ELISA Kit; Implantin ELISA Kit
Abbreviation ADAMTS5
Uniprot No. Q9R001
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAMTS5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 80-98  
1:2 Average % 92  
Range % 81-105  
1:4 Average % 94  
Range % 92-107  
1:8 Average % 95  
Range % 86-106  
Recovery
The recovery of mouse ADAMTS5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 84-98  
EDTA plasma (n=4) 96 92-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.423 2.414 2.419 2.236  
20 1.621 1.557 1.589 1.406  
10 0.904 0.925 0.915 0.732  
5 0.543 0.558 0.551 0.368  
2.5 0.382 0.372 0.377 0.194  
1.25 0.299 0.305 0.302 0.119  
0.625 0.247 0.256 0.252 0.069  
0 0.181 0.185 0.183    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Metalloproteinase that plays an important role in connective tissue organization, development, inflammation, arthritis, and cell migration. ADAMTS5 is an extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including aggrecan, versican, brevican and neurocan. Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its versican remodeling properties. Participates in the development of brown adipose tissue and browning of white adipose tissue
Gene References into Functions
  1. ADAMTS5 plays a functional role in development of brown adipose tissue and browning of white adipose tissue. PMID: 28702327
  2. Energy expenditure and thermogenesis were not significantly different between KO and WT ADAMTS5-J mice (in contrast to somewhat enhanced levels in ADAMTS5-P mice). Insulin sensitivity was improved in the ADAMTS5-J KO mice, and they were protected against non-alcoholic steatohepatitis in the DIO model PMID: 29293679
  3. Adamts5(-/-) mice were protected from hepatic mitochondrial dysfunction, as indicated by increased mitochondrial respiratory chain complex activity, higher ATP levels and higher expression of antioxidant enzymes. Absence of ADAMTS5 preserves liver integrity in a diet-induced obesity model. PMID: 27254774
  4. research emphasises the importance of ADAMTS5 expression in the control of influenza virus infection and highlights the potential for development of ADAMTS5-based therapeutic strategies to reduce morbidity and mortality PMID: 27855162
  5. TS5 protein functions to suppress glucose uptake in adipose-derived stromal cells and thereby inhibits the synthesis, and promotes the intracellular degradation of Acan and Vcan by an ADAMTS other than TS5. PMID: 25840345
  6. Data suggest that ADAMTS-5 oligomerization is required for full aggrecanase activity in vitro and in situ (as seen in knee joint of mouse model of inflammatory arthritis); thus, blocking oligomerization inhibits ADAMTS-5 activity. PMID: 26668318
  7. aggrecan and brevican proteolysis is compensated in Adamts4-/- or Adamts5-/- mice by ADAMTS proteoglycanase family members but a threshold of versican proteolysis is sensitive to the loss of a single ADAMTS proteoglycanase during spinal cord injury PMID: 25101296
  8. The present study reveals ADAMT-5 expression by mast cells(MCs) and that MC activation regulates the expression of the protease, thus implicating the ADAMT-5 of protease in MC function. PMID: 23154421
  9. Western blot analyses indicated that aggrecanase-generated proteoglycan fragments are produced after SCI. PMID: 23562508
  10. RelA/p65 is a potent transcriptional activator of ADAMTS5 in chondrocytes during osteoarthritis development. PMID: 23963448
  11. Repair of biomechanically compromised tendons exhibiting midsubstance chondroid accumulation requires ADAMTS5. PMID: 23754494
  12. this study identified, for the first time, several genes that have an ADAMTS-5-independent role in osteoarthritis(OA), identifying them as possible OA initiation candidates. PMID: 23436205
  13. The serine protease tissue plasminogen activator (tPA) and two matrix metalloproteinases, ADAMTS-4 and ADAMTS-5, were identified as Reelin cleaving enzymes. PMID: 23082219
  14. Versican processing by ADAMTS5 and ADAMTS15 contribute to muscle fiber formation. PMID: 23233679
  15. the first evidence implicating ADAMTS-5 in the regulation of proteoglycan turnover and lipoprotein retention in atherosclerosis. PMID: 22493487
  16. The role of ADAMTS5 in tendon is to remove pericellular and interfibrillar aggrecan to maintain the molecular architecture responsible for normal tissue function. PMID: 21928430
  17. Adamts5 induction in joint components other than cartilage, and its post-translational activation by PACE4 and/or furin may be important in the pathophysiology of arthritis. PMID: 21800360
  18. Matrilin-4 is processed by ADAMTS-5 in late Golgi vesicles present in growth plate chondrocytes of defined differentiation state PMID: 21539915
  19. a physiological function of ADAMTS5 in dermal fibroblasts is to maintain optimal versican content and PCM volume by continually trimming versican in hyaluronan-versican aggregates. PMID: 21828051
  20. Altered versican cleavage in ADAMTS5 deficient mice; a novel etiology of myxomatous valve disease PMID: 21749862
  21. ADAMTS5 ablation did not eliminate aggrecanase activity from the articular cartilage but blocked fibrosis and resulted in the accumulation of aggrecan in the articular cartilage. PMID: 21337391
  22. extracellular matrix degrading enzyme PMID: 11831030
  23. ADAMTS1, ADAMTS4, and ADAMTS5 are expressed in patterns that relate to the expression pattern of versican in granulosa cells of small follicles, expanded cumulus cell-oocyte complexes, and endothelial cells of the ovary. PMID: 15659705
  24. After surgically induced joint instability, there was significant reduction in the severity of cartilage destruction in the ADAMTS5 knockout mice compared with wild-type mice PMID: 15800624
  25. ADAMTS5 is the major aggrecanase in mouse cartilage, both in vitro and in a mouse model of inflammatory arthritis PMID: 15800625
  26. ADAMTS-5 is entirely responsible for cleavage in the interglobular domain, but cleavage in the chondroitin sulfate-rich region may be driven by ADAMTS-4 PMID: 17255106
  27. aggrecan loss with aggrecan processing in mice with single and double deletions of ADAMTS-4 and -5 activity (Deltacat) PMID: 17938173
  28. Deletion of ADAMTS-4/5 provided significant protection against proteoglycan degradation ex vivo and decreased the severity of murine osteoarthritis PMID: 17968948
  29. Data show that expression of Adamts5 during neuromuscular development and in smooth muscle cells coincides with the broadly distributed proteoglycan versican, an ADAMTS5 substrate. PMID: 19250981
  30. Fibroblast growth factor 2 is an intrinsic chondroprotective agent that suppresses ADAMTS-5 and delays cartilage degradation in murine osteoarthritis. PMID: 19565481
  31. The occurrence of less severe osteoarthritis-like cartilage destruction in both syndecan-4-deficient mice and syndecan-4-specific antibody-treated wild-type mice results from a marked decrease in ADAMTS-5 activity. PMID: 19684582
  32. show that combinatorial mouse alleles for the secreted metalloproteases Adamts5, Adamts20 (bt), and Adamts9 result in fully penetrant soft-tissue syndactyly. PMID: 19922873

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Subcellular Location Secreted, extracellular space, extracellular matrix
Database Links

KEGG: mmu:23794

STRING: 10090.ENSMUSP00000023611

UniGene: Mm.112933

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