Mouse Agrin(AGRN) ELISA kit

Instructions
Code CSB-EL001461MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name agrin
Alternative Names Agrn ELISA kit; AgrinAgrin [Cleaved into: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment ELISA kit; C90); Agrin C-terminal 22 kDa fragment ELISA kit; C22)] ELISA kit
Abbreviation AGRN
Uniprot No. A2ASQ1
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %81-92
1:2Average %102
Range %95-106
1:4Average %93
Range %85-96
1:8Average %97
Range %91-100
Recovery
The recovery of mouse AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8882-93
EDTA plasma (n=4)9890-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.257 2.166 2.212 2.038
101.361 1.378 1.370 1.196
50.858 0.884 0.871 0.697
2.50.572 0.578 0.575 0.401
1.250.447 0.433 0.440 0.266
0.6250.339 0.354 0.347 0.173
0.3120.248 0.232 0.240 0.066
00.176 0.172 0.174
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Isoform 1
Gene References into Functions
  1. The presence of agrin at locations with particular importance for the growth and stability of atherosclerotic plaques renders this molecule strategically positioned to influence plaque development and vulnerability PMID: 29405249
  2. Agrin induces clustering and activation of EphB1 receptors on developing erythroblasts, leading to the activation of alpha5beta1 integrins. PMID: 26990660
  3. identificaton of agrin, a component of neonatal extracellular matrix, as required for the full regenerative capacity of neonatal mouse hearts PMID: 28581497
  4. The presence of C-terminal fragment of agrin (CAF) in urine suggests either glomerular filtration or secretion into urine. PMID: 27380275
  5. Agrin strongly promotes chondrocyte differentiation and cartilage formation in vivo. PMID: 26290588
  6. Reveal novel roles for APP in regulating neuromuscular synapse formation through hetero-oligomeric interaction with LRP4 and agrin and thereby provide new insights into the molecular mechanisms that govern NMJ formation and maintenance. PMID: 23986861
  7. Agrin is frequently upregulated and important for oncogenic property of hepatocellular carcinoma. PMID: 25630468
  8. agrin contributes to barrier characteristics of brain endothelium. PMID: 25107608
  9. NMJs in agrin-deficient mice rapidly disappear after birth. Agrin is essential for postnatal NMJ maintenance, but not for their embryonic formation, and also for the postnatal, but not prenatal, midmuscle localization of postsynaptic specializations. PMID: 25368159
  10. reviews the evidence that agrin regulates synapse development, plasticity and signaling in the brain and discusses the evidence for the proposed mechanisms PMID: 22414531
  11. Using gamma-secretase drug inhibitors and presenilin mutants in embryonic fibroblasts, we found that a mature gamma-secretase complex was required to repress neurotrypsin expression and agrin cleavage. PMID: 24145027
  12. Neural agrin slows the degradation of surface AChR in cultured muscle cellsb via phosphorylation of the AChR beta subunit at Y390, stabilizing AChR at developing synapses & adult neuromuscular junctions. PMID: 23325468
  13. Agrin is Required for Maintenance of the Postsynaptic Apparatus. Agrin Loss Disrupts Nerve Terminal Organization PMID: 23056392
  14. Two classic synaptic signalling systems (neural agrin and neuregulin-1) converge upon MuSK to regulate postsynaptic differentiation. PMID: 22328506
  15. Agrin induces association of Chrna1 mRNA and nicotinic acetylcholine receptor in C2C12 myotubes. PMID: 22884571
  16. The suppression of agrin reduced hyperambulation and holeboard exploration, restored anxiety-like behavior (or reduced risk-taking behavior), improved prepulse inhibition, shortened the circadian period and increased brain Na(+) ,K(+) -ATPase activity. PMID: 22520507
  17. the cascade linking agrin to CLASP2-mediated microtubule capturing at the synaptic membrane is essential for the maintenance of a normal neuromuscular phenotype PMID: 22851317
  18. Agrin deficiency impairs monocytic cell maturation. PMID: 22517892
  19. Studies of neurotrypsin-deficient and agrin-overexpressing mice revealed that old-age sarcopenia also develops without neurotrypsin and is not prevented by elevated levels of agrin. Define neurotrypsin- and age-dependent sarcopenia as 2 distinct entities. PMID: 21885656
  20. The Agrn point mutation is a model for congenital myasthenic syndromes caused by Agrn mutations and potentially other related neuromuscular diseases. PMID: 21890498
  21. These results indicate that AGRIN is important for maintaining blood-brain barrier composition and that changes in Agrn expression (particularly vessel-associated AGRIN) influence Abeta homeostasis in mouse models of Alzheimer disease. PMID: 22082664
  22. Many clusters of acetylcholine receptors are observed in nestin/agrin double knockout-out mutants compared to significantly fewer in agrin single knockouts. PMID: 21832185
  23. The results support influence of agrin on AQP4, orthogonal arrays of particles and volume regulation. PMID: 20920487
  24. The agrin C22 construct, constitutes a well-folded, stable domain, while the deletion of 48 residues that correspond to strands beta1-beta4 of the agrin laminin G3 domain imposed by the agrin C15 construct leads to a misfolded protein. PMID: 21037280
  25. A role is proposed for agrin and laminin binding to alpha-dystrogycan at sensory synapses that places this activity upstream of neuregulin-ErbB signaling. PMID: 20213761
  26. the N-terminal half of agrin is involved in agrin's ability to inhibit neurite outgrowth. PMID: 11793362
  27. regulates neuronal responses to excitatory neurotransmitters PMID: 11817901
  28. Agrin plays an organizing role in the formation of sympathetic synapses PMID: 12221070
  29. Structures required for agrin signaling in CNS neurons are contained within a 20-kD COOH-terminal fragment of the protein. Distinct regions within agrin are responsible for receptor binding and activation. PMID: 12796478
  30. Expression of agrin in non-synaptic region varies greatly between different muscles in wild-type mice and its levels are altered in dystrophic muscle. PMID: 12798796
  31. Agrn becomes concentrated at neuroeffector and neuromuscular junctions in developing urinary bladder. PMID: 15711988
  32. agrin and perlecan cluster with laminin-2 to contribute to assembly of aneural AChR clusters that precede neural agrin release as well as affect later NMJ development PMID: 16219760
  33. agrin is able to rapidly and fully negate the laminin substrate clustering effect while inducing the rapid formation of new clusters. Acetylcholine receptor (AChR) half-life is not affected by agrin despite its role in inducing aggregation of AChRs. PMID: 16256100
  34. These data provide the first evidence for a specific role of TM-agrin in shaping the cytoskeleton of neurites in the developing nervous system. PMID: 16364653
  35. findings strongly indicate that agrin and alpha-dystroglycan mediate lymphocyte activation; furthermore, agrin-involved lymphocyte activation is mediated by alpha-dystroglycan PMID: 16394267
  36. These data demonstrate that, through its interaction with the alpha3 sodium-potassium ATPase, agrin regulates activity-dependent processes in neurons, providing a molecular framework for agrin action in the CNS. PMID: 16630822
  37. agrin likely triggers AChR clustering or maintains clusters through the coalescence of lipid rafts which play a pivotal role in the assembly of the postsynaptic membrane at the neuromuscular junction upon agrin signaling PMID: 16816402
  38. Data show that agrin is highly expressed by chondrocytes and localizes to the growth plate in wild-type mice and plays a critical role in normal skeletal growth. PMID: 17186266
  39. Transgenic mice overexpressing the heparan sulfate proteoglycan agrin have severe ocular dysgenesis. PMID: 17196957
  40. agrin is required for proper structural and functional development of an interneuronal synapse in vivo PMID: 17443806
  41. dysregulation of agrin processing is a good candidate for a pathogenetic mechanism underlying mental retardation PMID: 17586728
  42. Although agrin contributes significantly to the anionic charge to the GBM, both it and its charge are not needed for glomerular permselectivity. PMID: 17591961
  43. Agrin plays an important role in the formation and/or the maintenance of excitatory synapses in the brain. PMID: 17611272
  44. The acetylcholine receptor-associated protein rapsyn interacted with calpain in an agrin-dependent manner, and this interaction inhibited the protease activity of calpain. PMID: 17640526
  45. neurotrypsin exclusively cleaves glycanated variants of agrin; neurotrypsin-dependent cleavage of agrin was concentrated at synapses, where the most heavily glycanated variants of agrin predominate PMID: 18230682
  46. Our study reveals a potentially novel mechanism that regulates agrin/MuSK signaling cascade. PMID: 18272689
  47. At the postnatal neuromuscular synapse agrin signaling elevates the expression and targeting of rapsyn to the postsynaptic membrane, thereby packing more AChRs into stable, functionally-important AChR aggregates. PMID: 18506821
  48. Muscle-wide secretion of a miniaturized form of neural agrin rescues focal neuromuscular innervation in agrin mutant mice. PMID: 18685098
  49. These data are consistent with a role for Pofut1 in AChR aggregation during synaptogenesis via the regulation of the synaptogenic activity of muscle agrin. PMID: 18775496
  50. These experiments indicate that Lrp4 is the long-sought and elusive receptor for Agrin and has a critical role in activating MuSK and stimulating neuromuscular synapse formation. PMID: 18848351

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Subcellular Location Cell junction, synapse, Cell membrane, Single-pass type II membrane protein
Tissue Specificity Expressed in central nervous system (CNS) synapses such as in the cerebral cortex and hippocampus. Localizes to basal lamina of hippocampal blood vessels. Both (z+) and (z-) isoforms found in kidney, heart and cerebral vasculature.
Database Links

STRING: 10090.ENSMUSP00000137931

UniGene: Mm.273098

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