Mouse Apolipoprotein A-IV(APOA4) ELISA kit

Instructions
Code CSB-EL001916MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name apolipoprotein A-IV
Alternative Names Apoa4 ELISA Kit; Apolipoprotein A-IV ELISA Kit; Apo-AIV ELISA Kit; ApoA-IV ELISA Kit; Apolipoprotein A4 ELISA Kit
Abbreviation APOA4
Uniprot No. P06728
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1000Average %91
Range %87-98
1:2000Average %97
Range %93-104
1:4000Average %101
Range %96-104
1:8000Average %89
Range %83-96
Recovery
The recovery of mouse APOA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-94
EDTA plasma (n=4)10196-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
8002.635 2.755 2.695 2.561
4002.176 2.102 2.139 2.005
2001.605 1.622 1.614 1.480
1000.997 0.999 0.998 0.864
500.521 0.531 0.526 0.392
250.311 0.321 0.316 0.182
12.50.237 0.225 0.231 0.097
00.135 0.133 0.134
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Gene References into Functions
  1. This study uncovers an anti-sense lncRNA (APOA4-AS), which is co-expressed with APOA4, and concordantly and specifically regulates APOA4 expression both in vitro and in vivo with the involvement of HuR. PMID: 27131369
  2. Our findings may throw light on the function of ApoA4 in inflammatory responses and acute-phase reactions, as well as the development of SERPINA3 relative diseases. PMID: 28412351
  3. Very Low Density Lipoprotein (VLDL) assembly and CREBH activation play key roles in the response to hepatic steatosis by up-regulating apoA-IV and promoting assembly and secretion of larger, more triglyceride - enriched VLDL particles. PMID: 27655915
  4. It was suggested that increased ileal GPR119 is a potential mechanism by which GLP-1 secretion is enhanced in apoA-IV-/- mice. PMID: 26294669
  5. these data suggest that apoA-IV is not necessary for the metabolic improvements shown with VSG, but also suggest an interesting role for apoA-IV in regulating macronutrient preference and hepatic triglyceride levels. PMID: 25157093
  6. These results reveal ApoA-IV as a novel follicle-associated epithelium-specific marker especially in the upper small intestine of adult mice. PMID: 24838500
  7. transcriptional regulation of apolipoprotein A-IV by the transcription factor CREBH PMID: 24598141
  8. apoA-IV inhibits hepatic gluconeogenesis by decreasing Glc-6-Pase and PEPCK gene expression through NR1D1. PMID: 24311788
  9. Hepatic steatosis in mice induces hepatic apoA-IV expression, which in turn promotes lipoprotein particle expansion and reduces hepatic lipid burden without increasing the number of secreted atherogenic apoB-containing lipoprotein particles. PMID: 24030551
  10. Data indicate that plasma lipids, lipid absorption, and microsomal triglyceride transfer protein (MTP), FoxO1, and FoxA2 levels are lower at night and at mealtime in apoAIV-/- mice. PMID: 23729668
  11. a novel function of apoA-IV in the biogenesis of discrete HDL-A-IV particles with the participation of ABCA1 and LCAT PMID: 23132909
  12. peripheral apo AIV requires an intact CCK system and vagal afferents to activate neurons in the hindbrain to reduce food intake PMID: 23027805
  13. results suggest that apoA-IV may provide a therapeutic target for the regulation of glucose-stimulated insulin secretion and treatment of diabetes PMID: 22619326
  14. Data show that ApoA4 is a true target gene of LUMAN in bone marrow-derived DCs (BMDCs). PMID: 22209087
  15. These data suggest that Apoa4 has a previously unknown role in mediating the metabolism of chylomicrons, and therefore may be important in regulating plasma lipid metabolism. PMID: 22207575
  16. apoA-IV may play a unique role in integrating feeding behavior, intestinal lipid absorption, and energy storage PMID: 21840868
  17. ApoA-IV deficiency increases Abeta deposition and results in cognitive damage in the mouse model. PMID: 21356380
  18. ApoA-IV restriction to enterocyte is controlled by a new hormone-response element. PMID: 12105231
  19. Results provide the first direct support for the hypothesis that apolipoprotein A-IV is an endogenous anti-inflammatory protein. PMID: 15254593
  20. apo A-IV and apo A-V are positive acute-phase proteins in mouse HDL PMID: 15306172
  21. results demonstrate that apoA-IV is a direct ERRalpha target gene and suggest a function for ERRalpha in intestinal fat transport, a crucial step in energy balance PMID: 15466464
  22. the induction of apoA-IV expression in fasting and diabetes likely involves PGC-1 alpha-mediated coactivation of HNF-4 alpha in addition to glucocorticoid-dependent actions PMID: 16929032
  23. The hypothalamic apo A-IV is regulated by leptin, at least partially, via the STAT3 signaling pathway. PMID: 17363460
  24. Role for apo A-IV and CCK(1)R in PYY(3-36)-induced activation of vagal afferent pathway and inhibition of gastric emptying, but this is likely not pathway mediating effects of PYY(3-36) on food intake. PMID: 17641001
  25. the apoCIII enhancer contributes to the maintenance of an active chromatin subdomain of the apoAI/CIII/AIV genes, but not apoAV PMID: 18678879

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Subcellular Location Secreted
Protein Families Apolipoprotein A1/A4/E family
Tissue Specificity Secreted in plasma.
Database Links

KEGG: mmu:11808

STRING: 10090.ENSMUSP00000034585

UniGene: Mm.4533

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