Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
Apoa4; Apolipoprotein A-IV; Apo-AIV; ApoA-IV; Apolipoprotein A4
Species
Mus musculus (Mouse)
Expression Region
21-395aa
Target Protein Sequence
EVTSDQVANVVWDYFTQLSNNAKEAVEQFQKTDVTQQLSTLFQDKLGDASTYADGVHNKLVPFVVQLSGHLAQETERVKEEIKKELEDLRDRMMPHANKVTQTFGENMQKLQEHLKPYAVDLQDQINTQTQEMKLQLTPYIQRMQTTIKENVDNLHTSMMPLATNLKDKFNRNMEELKGHLTPRANELKATIDQNLEDLRRSLAPLTVGVQEKLNHQMEGLAFQMKKNAEELQTKVSAKIDQLQKNLAPLVEDVQSKVKGNTEGLQKSLEDLNRQLEQQVEEFRRTVEPMGEMFNKALVQQLEQFRQQLGPNSGEVESHLSFLEKSLREKVNSFMSTLEKKGSPDQPQALPLPEQAQEQAQEQAQEQVQPKPLES
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The synthesis of this Recombinant Mouse Apoa4 protein depends on the utilization of recombinant DNA technology to a large degree. DNA sequences that encoded the Apoa4 protein could be inserted into a vector and introduced into an expression host, Yeast, where it could be easily expressed in and purified from. The expression of this Apoa4 protein was at 21-395aa. N-terminal 6xHis tag was fused with this protein. The purity is 90%+ determined by SDS-PAGE.
Apoa4 is gene encoding a protein named apolipoprotein A-IV (Apoa4, Apo-AIV or ApoA-IV) or apolipoprotein A4. The protein encoded by this gene, a well-known short-term satiating protein, is highly present in by the gut and the brain in the presence of dietary lipids. Emerging evidence has reported that ApoA4 shows a S1P-binding property comparable to albumin, and ApoA4-bound S1P activated S1P receptors efficiently. ApoA4 can be a novel S1P carrier protein.