Mouse Apolipoprotein M(APOM) ELISA kit

Instructions
Code CSB-EL001947MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name apolipoprotein M
Alternative Names Apom ELISA kit; Ng20Apolipoprotein M ELISA kit; Apo-M ELISA kit; ApoM ELISA kit
Abbreviation APOM
Uniprot No. Q9Z1R3
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 86  
Range % 81-92  
1:2000 Average % 95  
Range % 88-99  
1:4000 Average % 93  
Range % 86-98  
1:8000 Average % 100  
Range % 96-107  
Recovery
The recovery of mouse APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 88-99  
EDTA plasma (n=4) 99 94-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.503 2.569 2.536 2.369  
50 2.278 2.207 2.243 2.076  
25 1.845 1.864 1.855 1.688  
12.5 1.317 1.329 1.323 1.156  
6.25 0.861 0.899 0.880 0.713  
3.12 0.582 0.592 0.587 0.420  
1.56 0.412 0.426 0.419 0.252  
0 0.168 0.166 0.167    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Sample type: Serum

Sample species: Mouse

Sample dilution: 1:2000

Review: I used the mouse ApoM ELISA kit to detect the apoM concentration in the serum. Easy to use. Total time about 4 hours.

By Anonymous

Target Data

Function Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid (By similarity).
Gene References into Functions
  1. apoM-S1P-S1PR1 signaling might underlie the pathogenesis of ALI and apoM could have physiological benefits to alleviate LPS-induced ALI. PMID: 29260347
  2. These S1P-induced enhancements in growth factors and chemotactic cytokines in retinal pigment epithelium cells were significantly inhibited by ApoM treatment. Additionally, in vivo experiments using a laser-induced choroidal neovascularization (CNV) murine model demonstrated that intravitreal ApoM injection significantly reduced the progression of CNV formation. PMID: 29301231
  3. This study highlights the complexity of Apom/S1P in atherosclerosis and challenges the notion that the Apom/S1P complex is anti-atherogenic, at least in Apoe-deficient mice. PMID: 28866363
  4. This suggests that the autophagy dysfunction caused by the deficiency of ApoM is an important factor in hepatic steatosis (triglyceride accumulation). ApoM plays a key role in normal autophagy activity in the liver and thereby further regulates the metabolism of liver lipids, particularly triglycerides. PMID: 29288662
  5. HDL facilitates S1P efflux from erythrocytes by both apoM-dependent and apoM-independent mechanisms. Moreover, apoM facilitates tubular reabsorption of S1P from the urine, however, with no impact on S1P plasma concentrations. PMID: 24950692
  6. The study suggests that vascular leakage of albumin-sized particles in ApoM deficiency is S1P- and S1P1-dependent and this dependency exacerbates the response to inflammatory stimuli. PMID: 26956418
  7. apoM might facilitate the maintenance of CD4(+) T-lymphocytes or could modify the T-lymphocytes subgroups in murine spleen PMID: 26543853
  8. Upon immune stimulation, Apom(-/-) mice developed more severe experimental autoimmune encephalomyelitis, characterized by increased lymphocytes in the central nervous system and breakdown of the blood-brain barrier PMID: 26053123
  9. LDL receptor and ApoE have roles in the clearance of ApoM-associated sphingosine 1-phosphate PMID: 25505264
  10. ApoM augmented insulin secretion by maintaining the S1P concentration under both in vivo and in vitro conditions. PMID: 24814049
  11. The present data indicate that the plasma apo-M levels modulate the ability of plasma to mobilize cellular cholesterol, whereas apo-M has no major effect on the excretion of cholesterol into feces. PMID: 24046869
  12. ApoM can bind oxidized phospholipids, increasing the antioxidant effect of HDL. PMID: 22204862
  13. Results show that apoM, by delivering S1P to the S1P(1) receptor on endothelial cells, is a vasculoprotective constituent of HDL. PMID: 21606363
  14. After refolding from inclusion bodies, the crystal structure of apoM (reported here at 2.5 A resolution) displays a novel yet unprecedented seven-stranded beta-barrel structure. PMID: 20932978
  15. apoM mainly associates with HDL in normal mice but also with the pathologically increased lipoprotein fraction in genetically modified mice; decreased apoM levels in apoA-I-deficient mice suggest a connection between apoM and apoA-I metabolism. PMID: 15102887
  16. ApoM transcripts were detectable in mouse embryos from day 7.5 to day 18.5 PMID: 15147633
  17. in both liver and kidney, expression of apoM was significantly lower in leptin deficient ob/ob mice and in leptin-receptor deficient db/db mice than in control mice PMID: 15358114
  18. Overexpression of apoM in Ldlr(-/-) mice protected against atherosclerosis when the mice were challenged with a cholesterol-enriched diet. PMID: 15793583
  19. Megalin-mediated endocytosis in kidney proximal tubules prevents apoM excretion in the urine. PMID: 16099815
  20. Based on these results, we suggest that insulin regulates apoM synthesis in vivo and, therefore, that the reduction of apoM expression is a general phenomenon in diabetes models. PMID: 16516154
  21. In the setting of low density lipoprotein receptor deficiency, apoM-Tg mice with approximately 2-fold increased plasma apoM concentrations developed smaller atherosclerotic lesions than controls. PMID: 18006500
  22. Apolipoprotein M is a negative acute response protein that decreases during infection and inflammation PMID: 18054359
  23. Foxa2 activity increases plasma high density lipoprotein levels by regulating apolipoprotein M PMID: 18381283

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Subcellular Location Secreted
Protein Families Calycin superfamily, Lipocalin family
Tissue Specificity Expressed by the liver; secreted in plasma.
Database Links

KEGG: mmu:55938

STRING: 10090.ENSMUSP00000025249

UniGene: Mm.2161

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