Recombinant Mouse Apolipoprotein M (Apom)

Code CSB-EP001947MO
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Source E.coli
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Code CSB-EP001947MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001947MO
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Source Baculovirus
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Code CSB-MP001947MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Apom
Uniprot No.
Alternative Names
Apom; Ng20Apolipoprotein M; Apo-M; ApoM
Species
Mus musculus (Mouse)
Expression Region
1-190
Target Protein Sequence
MFHQVWAALL SLYGLLFNSM NQCPEHSQLT ALGMDDTETP EPHLGLWYFI AGAASTTEEL ATFDPVDNIV FNMAAGSAPR QLQLRATIRT KSGVCVPRKW TYRLTEGKGN MELRTEGRPD MKTDLFSSSC PGGIMLKETG QGYQRFLLYN RSPHPPEKCV EEFQSLTSCL DFKAFLVTPR NQEACPLSSK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
Gene References into Functions
  1. apoM-S1P-S1PR1 signaling might underlie the pathogenesis of ALI and apoM could have physiological benefits to alleviate LPS-induced ALI. PMID: 29260347
  2. These S1P-induced enhancements in growth factors and chemotactic cytokines in retinal pigment epithelium cells were significantly inhibited by ApoM treatment. Additionally, in vivo experiments using a laser-induced choroidal neovascularization (CNV) murine model demonstrated that intravitreal ApoM injection significantly reduced the progression of CNV formation. PMID: 29301231
  3. This study highlights the complexity of Apom/S1P in atherosclerosis and challenges the notion that the Apom/S1P complex is anti-atherogenic, at least in Apoe-deficient mice. PMID: 28866363
  4. This suggests that the autophagy dysfunction caused by the deficiency of ApoM is an important factor in hepatic steatosis (triglyceride accumulation). ApoM plays a key role in normal autophagy activity in the liver and thereby further regulates the metabolism of liver lipids, particularly triglycerides. PMID: 29288662
  5. HDL facilitates S1P efflux from erythrocytes by both apoM-dependent and apoM-independent mechanisms. Moreover, apoM facilitates tubular reabsorption of S1P from the urine, however, with no impact on S1P plasma concentrations. PMID: 24950692
  6. The study suggests that vascular leakage of albumin-sized particles in ApoM deficiency is S1P- and S1P1-dependent and this dependency exacerbates the response to inflammatory stimuli. PMID: 26956418
  7. apoM might facilitate the maintenance of CD4(+) T-lymphocytes or could modify the T-lymphocytes subgroups in murine spleen PMID: 26543853
  8. Upon immune stimulation, Apom(-/-) mice developed more severe experimental autoimmune encephalomyelitis, characterized by increased lymphocytes in the central nervous system and breakdown of the blood-brain barrier PMID: 26053123
  9. LDL receptor and ApoE have roles in the clearance of ApoM-associated sphingosine 1-phosphate PMID: 25505264
  10. ApoM augmented insulin secretion by maintaining the S1P concentration under both in vivo and in vitro conditions. PMID: 24814049
  11. The present data indicate that the plasma apo-M levels modulate the ability of plasma to mobilize cellular cholesterol, whereas apo-M has no major effect on the excretion of cholesterol into feces. PMID: 24046869
  12. ApoM can bind oxidized phospholipids, increasing the antioxidant effect of HDL. PMID: 22204862
  13. Results show that apoM, by delivering S1P to the S1P(1) receptor on endothelial cells, is a vasculoprotective constituent of HDL. PMID: 21606363
  14. After refolding from inclusion bodies, the crystal structure of apoM (reported here at 2.5 A resolution) displays a novel yet unprecedented seven-stranded beta-barrel structure. PMID: 20932978
  15. apoM mainly associates with HDL in normal mice but also with the pathologically increased lipoprotein fraction in genetically modified mice; decreased apoM levels in apoA-I-deficient mice suggest a connection between apoM and apoA-I metabolism. PMID: 15102887
  16. ApoM transcripts were detectable in mouse embryos from day 7.5 to day 18.5 PMID: 15147633
  17. in both liver and kidney, expression of apoM was significantly lower in leptin deficient ob/ob mice and in leptin-receptor deficient db/db mice than in control mice PMID: 15358114
  18. Overexpression of apoM in Ldlr(-/-) mice protected against atherosclerosis when the mice were challenged with a cholesterol-enriched diet. PMID: 15793583
  19. Megalin-mediated endocytosis in kidney proximal tubules prevents apoM excretion in the urine. PMID: 16099815
  20. Based on these results, we suggest that insulin regulates apoM synthesis in vivo and, therefore, that the reduction of apoM expression is a general phenomenon in diabetes models. PMID: 16516154
  21. In the setting of low density lipoprotein receptor deficiency, apoM-Tg mice with approximately 2-fold increased plasma apoM concentrations developed smaller atherosclerotic lesions than controls. PMID: 18006500
  22. Apolipoprotein M is a negative acute response protein that decreases during infection and inflammation PMID: 18054359
  23. Foxa2 activity increases plasma high density lipoprotein levels by regulating apolipoprotein M PMID: 18381283

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Subcellular Location
Secreted.
Protein Families
Calycin superfamily, Lipocalin family
Tissue Specificity
Expressed by the liver; secreted in plasma.
Database Links
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