Mouse Claudin-5(CLDN5) ELISA kit

Code CSB-EL005507MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse CLDN5 ELISA Kit was designed for the quantitative measurement of Mouse CLDN5 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.

Target Name claudin 5
Alternative Names Cldn5; Bec1; Claudin-5; Brain endothelial cell clone 1 protein; Lung-specific membrane protein
Abbreviation CLDN5
Uniprot No. O54942
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.68 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLDN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 101
Range % 95-104
1:2 Average % 95
Range % 90-99
1:4 Average % 92
Range % 87-98
1:8 Average % 92
Range % 86-97
Recovery
The recovery of mouse CLDN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 83-92
EDTA plasma (n=4) 93 87-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1200 2.312 2.422 2.367 2.240
600 1.846 1.966 1.906 1.779
300 1.261 1.229 1.245 1.118
150 0.754 0.769 0.762 0.635
75 0.443 0.456 0.450 0.323
37.5 0.271 0.281 0.276 0.149
18.75 0.208 0.202 0.205 0.078
0 0.126 0.128 0.127  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Gene References into Functions
  1. This study demonstrated that the claudin-5(+) leukocytes in the central nervous system during neuroinflammation: a novel role for endothelial-derived extracellular vesicles. PMID: 27852330
  2. Claudin 5 in a murine model of allergic asthma: Its implication and response to steroid treatment PMID: 26409663
  3. while MMP-9 is not essential for hypoxic-induced cerebral angiogenesis, it plays an important role in post-hypoxic vascular pruning by degrading laminin and claudin-5 PMID: 25812799
  4. Studied if circulating TGFbeta1 drives changes in tight junction protein expression and MMP9 activity following acute liver failure; Mouse brain endothelial cells were treated with recombinant rTGFbeta1 and MMP9 and claudin-5 expression was assessed. PMID: 26006017
  5. In transgenic mice carrying mutated SOD1 genes, a disrupted blood-spinal cord barrier as well as decreased levels of tight junction proteins ZO-1, occludin, and claudin-5 were detected. PMID: 25853911
  6. The aim of the present study was to further characterize the estrogen-responsive elements of claudin-5 promoter. PMID: 24846172
  7. Two independent pathways triggered by PI3K mediate early and late loss of paracellular claudin-5 expression. PMID: 25281324
  8. Comparative modelling of intramolecular interfaces in the transmembrane region of claudins led to a complete Cldn5 model. PMID: 25174580
  9. silencing of claudin-5 did significantly attenuate simvastatin-mediated endothelial cell barrier protection in response to thrombin PMID: 24028293
  10. Claudin 5 showed identical mRNA expression levels in the brains of adult and aged mice. PMID: 22120415
  11. TNFalpha acts through NFkappaB signaling and requires a conserved promoter region for the down-regulation of Cldn5 expression. PMID: 22138107
  12. In female rats, increasing age and 17beta-estradiol treatment alters the expression of ERalpha and distinct blood-brain barrier tight-junction protein isoforms (occludin and claudin-5) without altering functional paracellular permeability. PMID: 21192956
  13. These findings suggest that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels. PMID: 21271259
  14. This study sought to determine the cell signaling mechanism controlling endothelial CLDN5 expression during acute lung injury. PMID: 20525806
  15. envision that substances interfering with Cldn5-mediated paracellular barrier tightening could assist an improved drug delivery into the brain because Cldn5 is essential for blood-brain barrier integrity. PMID: 20080584
  16. Describe claudin-5 as a novel estrogen target in vascular endothelium and show in vivo (brain endothelium) and in vitro (brain and heart endothelium) effects of estrogen on claudin-5 levels. PMID: 19910637
  17. tight junctions in dermal vascular endothelial cells are composed of claudin-5. PMID: 12823443
  18. claudin-5 is specifically altered in utrophin/dystrophin-deficient (double knockout, dko hearts, suggesting that alterations of the lateral membranes of cardiomyocytes PMID: 15698839
  19. Measured changes in claudin5 after cardiac arrest in mouse lung in relation to warm ischemia time and lung injury. PMID: 18281348
  20. Amyotrophic lateral sclerosis-linked superoxide dismutase 1 (SOD1) mutants with different biochemical characteristics disrupted the blood-spinal cord barrier in mice by reducing the levels of the tight junction protein claudin-5. PMID: 18344992
  21. Hydrodynamic tail vein injection of Cldn5 siRNA attenuates Cldn5 expression. PMID: 18509865
  22. Tissue-specific regulation of the claudin-5 gene via glucocorticoids and a high vulnerability of claudin-5 to TNF alpha. PMID: 18996436
  23. the pro-inflammatory chemokine CCL2 mediates brain endothelial barrier disruption during CNS inflammation PMID: 19423710
  24. CLDN5 is expressed in the seminiferous epithelium, appears to be regulated by multiple influences, and contributes to blood-testis barrier function. PMID: 19571261
  25. Disruptions of occludin and claudin-5 in brain endothelial cells in vitro and in brains of mice with acute liver failure. PMID: 19821483

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Subcellular Location Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
Protein Families Claudin family
Tissue Specificity Widely expressed with highest levels in the lung.
Database Links

KEGG: mmu:12741

STRING: 10090.ENSMUSP00000041925

UniGene: Mm.22768

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