Product Details

Description

This Mouse CLDN5 ELISA Kit was designed for the quantitative measurement of Mouse CLDN5 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.

Target Name

claudin 5

Alternative Names

Cldn5; Bec1; Claudin-5; Brain endothelial cell clone 1 protein; Lung-specific membrane protein

Abbreviation

CLDN5

Uniprot No.

O54942

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

18.75 pg/mL-1200 pg/mL

Sensitivity

4.68 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLDN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-104
1:2	Average %	95
	Range %	90-99
1:4	Average %	92
	Range %	87-98
1:8	Average %	92
	Range %	86-97

Recovery

The recovery of mouse CLDN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	83-92
EDTA plasma (n=4)	93	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.312	2.422	2.367	2.240
600	1.846	1.966	1.906	1.779
300	1.261	1.229	1.245	1.118
150	0.754	0.769	0.762	0.635
75	0.443	0.456	0.450	0.323
37.5	0.271	0.281	0.276	0.149
18.75	0.208	0.202	0.205	0.078
0	0.126	0.128	0.127

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Gene References into Functions	This study demonstrated that the claudin-5(+) leukocytes in the central nervous system during neuroinflammation: a novel role for endothelial-derived extracellular vesicles. PMID: 27852330 Claudin 5 in a murine model of allergic asthma: Its implication and response to steroid treatment PMID: 26409663 while MMP-9 is not essential for hypoxic-induced cerebral angiogenesis, it plays an important role in post-hypoxic vascular pruning by degrading laminin and claudin-5 PMID: 25812799 Studied if circulating TGFbeta1 drives changes in tight junction protein expression and MMP9 activity following acute liver failure; Mouse brain endothelial cells were treated with recombinant rTGFbeta1 and MMP9 and claudin-5 expression was assessed. PMID: 26006017 In transgenic mice carrying mutated SOD1 genes, a disrupted blood-spinal cord barrier as well as decreased levels of tight junction proteins ZO-1, occludin, and claudin-5 were detected. PMID: 25853911 The aim of the present study was to further characterize the estrogen-responsive elements of claudin-5 promoter. PMID: 24846172 Two independent pathways triggered by PI3K mediate early and late loss of paracellular claudin-5 expression. PMID: 25281324 Comparative modelling of intramolecular interfaces in the transmembrane region of claudins led to a complete Cldn5 model. PMID: 25174580 silencing of claudin-5 did significantly attenuate simvastatin-mediated endothelial cell barrier protection in response to thrombin PMID: 24028293 Claudin 5 showed identical mRNA expression levels in the brains of adult and aged mice. PMID: 22120415 TNFalpha acts through NFkappaB signaling and requires a conserved promoter region for the down-regulation of Cldn5 expression. PMID: 22138107 In female rats, increasing age and 17beta-estradiol treatment alters the expression of ERalpha and distinct blood-brain barrier tight-junction protein isoforms (occludin and claudin-5) without altering functional paracellular permeability. PMID: 21192956 These findings suggest that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels. PMID: 21271259 This study sought to determine the cell signaling mechanism controlling endothelial CLDN5 expression during acute lung injury. PMID: 20525806 envision that substances interfering with Cldn5-mediated paracellular barrier tightening could assist an improved drug delivery into the brain because Cldn5 is essential for blood-brain barrier integrity. PMID: 20080584 Describe claudin-5 as a novel estrogen target in vascular endothelium and show in vivo (brain endothelium) and in vitro (brain and heart endothelium) effects of estrogen on claudin-5 levels. PMID: 19910637 tight junctions in dermal vascular endothelial cells are composed of claudin-5. PMID: 12823443 claudin-5 is specifically altered in utrophin/dystrophin-deficient (double knockout, dko hearts, suggesting that alterations of the lateral membranes of cardiomyocytes PMID: 15698839 Measured changes in claudin5 after cardiac arrest in mouse lung in relation to warm ischemia time and lung injury. PMID: 18281348 Amyotrophic lateral sclerosis-linked superoxide dismutase 1 (SOD1) mutants with different biochemical characteristics disrupted the blood-spinal cord barrier in mice by reducing the levels of the tight junction protein claudin-5. PMID: 18344992 Hydrodynamic tail vein injection of Cldn5 siRNA attenuates Cldn5 expression. PMID: 18509865 Tissue-specific regulation of the claudin-5 gene via glucocorticoids and a high vulnerability of claudin-5 to TNF alpha. PMID: 18996436 the pro-inflammatory chemokine CCL2 mediates brain endothelial barrier disruption during CNS inflammation PMID: 19423710 CLDN5 is expressed in the seminiferous epithelium, appears to be regulated by multiple influences, and contributes to blood-testis barrier function. PMID: 19571261 Disruptions of occludin and claudin-5 in brain endothelial cells in vitro and in brains of mice with acute liver failure. PMID: 19821483 Show More Hide All
Subcellular Location	Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
Protein Families	Claudin family
Tissue Specificity	Widely expressed with highest levels in the lung.
Database Links	KEGG: mmu:12741 STRING: 10090.ENSMUSP00000041925 UniGene: Mm.22768

CloseMessage

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
Check Code：

Code	CSB-EL005507MO
Size	96T,5×96T,10×96T
Trial Size
Have Questions?	Leave a Message or Start an on-line Chat

Mouse Claudin-5(CLDN5) ELISA kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

CloseMessage