Mouse Cystatin-F(CST7) ELISA kit

Instructions
Code CSB-EL006095MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cystatin F (leukocystatin)
Alternative Names Cst7Cystatin-F ELISA Kit; Cystatin-7 ELISA Kit; Cystatin-like metastasis-associated protein ELISA Kit; CMAP ELISA Kit; Leukocystatin ELISA Kit
Abbreviation CST7
Uniprot No. O89098
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CST7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 84-93
1:2 Average % 100
Range % 95-105
1:4 Average % 87
Range % 83-90
1:8 Average % 104
Range % 100-107
Recovery
The recovery of mouse CST7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 86 83-89
EDTA plasma (n=4) 95 91-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.547 2.601 2.574 2.452
5 2.120 2.032 2.076 1.954
2.5 1.394 1.402 1.398 1.276
1.25 0.820 0.805 0.813 0.691
0.625 0.517 0.531 0.524 0.402
0.312 0.325 0.326 0.326 0.204
0.156 0.239 0.242 0.241 0.119
0 0.121 0.123 0.122  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
Gene References into Functions
  1. High CST7 expression is associated with Alzheimer's disease. PMID: 28904096
  2. Our results validate cystatin F as a useful biomarker of early pathogenesis in experimental models of prion disease, and point to unexpected species-specific differences in the transcriptional responses to prion infections. PMID: 28178353
  3. During the active remyelinating phase, both CysF knockdown (CysFKD) and microglial-selective CatC overexpression (CatCOE) showed a worsening of the demyelination in Plp(4e/-) transgenic mice. Conversely, during the chronic demyelinating phase, CatC knockdown (CatCKD) ameliorated the demyelination. Our results suggest that the balance between CatC and CysF expression controls the demyelination and remyelination process. PMID: 28251676
  4. The cystatin F expression in the activated microglia is closely associated with the effect of the A2A receptors, which may be related to the neuroinflammatory responses occurring during the pathological process. PMID: 23285090
  5. an inhibitory activity other than cystatin F quenches CatL activity in activated macrophages PMID: 21956111
  6. Expression of cystatin F indicates ongoing demyelination and remyelination; absence of cystatin F expression indicates cessation of remyelination in the demyelinating area. PMID: 21344476

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Subcellular Location Secreted
Protein Families Cystatin family
Database Links

KEGG: mmu:13011

STRING: 10090.ENSMUSP00000086606

UniGene: Mm.12965

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