Mouse D-Lactate Dehydrogenase,D-LDH ELISA Kit

Code CSB-E11723m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
lactate dehydrogenase D
Alternative Names
Ldhd ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit; mitochondrial ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; EC ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
4.69 mU/mL-300 mU/mL
1.17 mU/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-89  
1:2 Average % 93  
Range % 90-96  
1:4 Average % 87  
Range % 83-90  
1:8 Average % 98  
Range % 94-102  
The recovery of mouse D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-95  
EDTA plasma (n=4) 104 101-107  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected  
300 2.278 2.304 2.291 2.111  
150 1.422 1.500 1.461 1.281  
75 0.932 0.921 0.927 0.747  
37.5 0.623 0.624 0.624 0.444  
18.75 0.385 0.394 0.390 0.210  
9.38 0.287 0.275 0.281 0.101  
4.69 0.242 0.231 0.237 0.057  
0 0.181 0.179 0.180    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Mouse LDHD ELISA Kit was designed for the quantitative measurement of Mouse LDHD protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.69 mU/mL-300 mU/mL and the sensitivity is 1.17 mU/mL.

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Target Background

(From Uniprot)
Involved in D-lactate, but not L-lactate catabolic process.
Gene References into Functions
  1. identification and characterization of human and mouse D-lactate dehydrogenase (NAD) [D-LDH] PMID: 12127981
Subcellular Location
Protein Families
FAD-binding oxidoreductase/transferase type 4 family
Tissue Specificity
Readily detected in liver and kidney, with a weaker signal observed in heart, skeletal muscle, stomach, brain, and lung.
Database Links
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