Mouse D-Lactate Dehydrogenase,D-LDH ELISA Kit

Instructions
Code CSB-E11723m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lactate dehydrogenase D
Alternative Names Ldhd ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit; mitochondrial ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; EC 1.1.2.4 ELISA Kit
Abbreviation LDHD
Uniprot No. Q7TNG8
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 4.69 mU/mL-300 mU/mL
Sensitivity 1.17 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-89  
1:2 Average % 93  
Range % 90-96  
1:4 Average % 87  
Range % 83-90  
1:8 Average % 98  
Range % 94-102  
Recovery
The recovery of mouse D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-95  
EDTA plasma (n=4) 104 101-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected  
300 2.278 2.304 2.291 2.111  
150 1.422 1.500 1.461 1.281  
75 0.932 0.921 0.927 0.747  
37.5 0.623 0.624 0.624 0.444  
18.75 0.385 0.394 0.390 0.210  
9.38 0.287 0.275 0.281 0.101  
4.69 0.242 0.231 0.237 0.057  
0 0.181 0.179 0.180    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Gene References into Functions
  1. identification and characterization of human and mouse D-lactate dehydrogenase (NAD) [D-LDH] PMID: 12127981
Subcellular Location Mitochondrion
Protein Families FAD-binding oxidoreductase/transferase type 4 family
Tissue Specificity Readily detected in liver and kidney, with a weaker signal observed in heart, skeletal muscle, stomach, brain, and lung.
Database Links

KEGG: mmu:52815

STRING: 10090.ENSMUSP00000068086

UniGene: Mm.271578

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