Mouse Desmosine ELISA Kit

Code CSB-E14196m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
0.78 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Desmosine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:10 Average % 85  
Range % 81-92  
1:20 Average % 96  
Range % 91-103  
1:40 Average % 103  
Range % 97-107  
1:80 Average % 98  
Range % 92-102  
The recovery of mouse Desmosine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 82-96  
EDTA plasma (n=4) 104 96-108  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.495 2.594 2.545 2.355  
100 1.804 1.903 1.854 1.664  
50 1.128 1.148 1.138 0.948  
25 0.670 0.661 0.666 0.476  
12.5 0.441 0.422 0.432 0.242  
6.25 0.314 0.305 0.310 0.120  
3.12 0.255 0.257 0.256 0.066  
0 0.189 0.190 0.190    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Mouse Desmosine ELISA Kit was designed for the quantitative measurement of Mouse Desmosine protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

Customer Reviews and Q&A

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Cluld you pls offer me the below information:1. Does this kit also detect Isodamosine, or just Desmosine?
2. What is the range of desmosine in control/normal human plasma?

Thanks for your inquiry.
The kit csb-e14196m uses the double antibody sandwich principle to detect the polymerization form of Desmosine.
We make detection on normal serum and plasma sample, 1:200 dilution, getting the value is 12.5--50pg/ml, then the final concentration is 2.5-10ng/ml.Pls let me know if you have any further questions. Thank you.

I have already prepared samples by homogenizing using thermo fisher’s MPER buffer (see link below)
The only content information I can find is “Formulation
Proprietary detergent in 25mM bicine buffer, pH 7.6”
Here i would like to know if we can use these samples with your ELISA. We appear to believe that PBS and freeze thaw alone may not be sufficient to break up the elastin fibers?
Please send your comments.

Thanks for your inquiry.
From the manual of the buffer we think there will be no problem in theory.
We suggest you do a pretest first.
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