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| Code | CSB-E14196m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Desmosine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 85 | ||||
| Range % | 81-92 | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:40 | Average % | 103 | ||||
| Range % | 97-107 | |||||
| 1:80 | Average % | 98 | ||||
| Range % | 92-102 | |||||
| The recovery of mouse Desmosine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 82-96 | ||||
| EDTA plasma (n=4) | 104 | 96-108 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Mouse Desmosine ELISA Kit was designed for the quantitative measurement of Mouse Desmosine protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .
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Cluld you pls offer me the below information:1. Does this kit also detect Isodamosine, or just Desmosine?
2. What is the range of desmosine in control/normal human plasma?
I have already prepared samples by homogenizing using thermo fisher’s MPER buffer (see link below)
https://www.thermofisher.com/order/catalog/product/78501
The only content information I can find is “Formulation
Proprietary detergent in 25mM bicine buffer, pH 7.6”
Here i would like to know if we can use these samples with your ELISA. We appear to believe that PBS and freeze thaw alone may not be sufficient to break up the elastin fibers?
Please send your comments.
