Mouse Galectin-9(LGALS9) ELISA kit

Instructions
Code CSB-EL012895MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lectin, galactoside-binding, soluble, 9
Alternative Names Lgals9 ELISA Kit; Galectin-9 ELISA Kit; Gal-9 ELISA Kit
Abbreviation LGALS9
Uniprot No. O08573
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGALS9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 86-98
1:2 Average % 99
Range % 92-107
1:4 Average % 95
Range % 92-98
1:8 Average % 89
Range % 82-94
Recovery
The recovery of mouse LGALS9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 86-93
EDTA plasma (n=4) 101 95-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.339 2.314 2.327 2.212
500 1.853 1.919 1.886 1.771
250 1.355 1.356 1.356 1.241
125 0.742 0.724 0.733 0.618
62.5 0.423 0.411 0.417 0.302
31.2 0.289 0.285 0.287 0.172
15.6 0.222 0.233 0.228 0.113
0 0.115 0.114 0.115  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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 Q&A
Q:

Please let us know the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry!The sulfuric acid content of [stop solution] is 4N.

Target Data

Function Binds galactosides (By similarity). Has high affinity for the Forssman pentasaccharide (By similarity). Ligand for HAVCR2/TIM3 (By similarity). Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death (By similarity). Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth
Gene References into Functions
  1. galectin-9 mediates the close association of IgM and CD22. PMID: 30120235
  2. m4-1BBL and Gal-9 act together to aid aggregation of m4-1BB monomers to efficiently initiate m4-1BB signaling. PMID: 29242193
  3. this study shows that impaired Gal-9 dysregulates the blood mononuclear cells-induced Th1/Th2 imbalance in abortion-prone matings PMID: 29651447
  4. Gal9 may be a new immunomodulatory target for macrophages. PMID: 28990062
  5. these findings reveal a critical role for Tim-3-Gal-9 signaling-mediated immunoregulation by pNK cells in maternal-fetal immune tolerance and suggest that Tim-3 abundance on pNK cells is a potential biomarker for recurrent miscarriage diagnosis. PMID: 28951537
  6. in a model of experimental autoimmune encephalomyelitis, we show that an agonist of DR3 suppressed disease, correlating with expansion of CD4+Foxp3+ Tregs, and this protective effect was lost in Galectin-9-/- mice PMID: 28877989
  7. Galectin 9 is a novel dectin 1 ligand in pancreatic ductal adenocarcinoma. Upon disruption of the dectin 1-galectin 9 axis, CD4(+) and CD8(+) T cells, which are dispensable for PDA progression in hosts with an intact signaling axis, become reprogrammed into indispensable mediators of anti-tumor immunity. PMID: 28394331
  8. The Gal-9/Tim-3 signal is important for the regulation of decidua NK cells function, which is beneficial for the maintenance of a normal pregnancy. PMID: 25578313
  9. present in the spongiotrophoblast layer of the haemochorial placenta PMID: 26278059
  10. These results indicate the possibility that cooperative binding of oligosaccharide and neighboring polypeptide structures of monoclonal IgE to galectin-9 affects the overall affinity and specificity of the IgE-lectin interaction. PMID: 26582205
  11. galectin-9 functions as a novel alarmin by augmenting the inflammatory response in sepsis development during pulmonary F. novicida infection. PMID: 25898318
  12. Gal-9 has a role in the maintenance of hepatic homeostasis and preventing liver ischemia-reperfusion injury PMID: 25931247
  13. Gal-9, a natural TIM-3 ligand, was produced primarily by and released from ischemia-reperfusion-stressed hepatocytes, both in vivo and in vitro. PMID: 25450716
  14. Galectin-9 functions as an astrocyte-microglia communication signal and promotes cytokine production from microglia in a Tim-3 independent manner. PMID: 25158758
  15. galectin-9, in addition to being an effector molecule for regulatory T cells cells, acts synergistically with TGF-beta to enforce iTreg cell differentiation and maintenance. PMID: 25065622
  16. Galectin-9 is a suppressor of T and B cells and predicts the immune modulatory potential of mesenchymal stromal cell preparations. PMID: 24083426
  17. action of agonist anti-4-1BB in suppressing autoimmune and allergic inflammation was completely dependent on Galectin-9 (Gal-9). Gal-9 directly bound to 4-1BB, in a site distinct from the binding site of antibodies and the natural ligand of 4-1BB PMID: 24958847
  18. Tim-3/galectin-9 pathway plays a critical role in the homeostasis of hepatic Tregs through the elimination induction in Teffs and the inhibition of IFN-gamma release. PMID: 24333756
  19. Gal-9 expressed on corneal endothelial cells protects them from destruction by allo-reactive T cells within the cornea. PMID: 23667648
  20. contributes to the inducible immunomodulatory functions of mesenchymal stromal cells PMID: 23817958
  21. Gal-9 is induced in astrocytes by TNF via the JNK/c-Jun pathway. PMID: 23836896
  22. Galectin-9 impairs the function of NK cells, including cytotoxicity and cytokine production. PMID: 23408620
  23. These results show a differential regulation of Lgals9 isoform expression during normal and pathological pregnancies and designate Lgals9 as a potential marker for adverse pregnancy outcomes. PMID: 23242525
  24. CD4 T cells expressing Gal-9 on the cell surface (Gal-9(+) Th cells) secrete Gal-9 upon T cell receptor (TCR) stimulation. PMID: 23144904
  25. Gal-9 directly induces Tim-3+ natural killer [NK]T cell apoptosis and contributes to the depletion of NKT cells in diet-induced steatosis; Gal-9 also interacts with Tim-3-expressing Kupffer cells to induce secretion of interleukin (IL)-15. PMID: 23296703
  26. These studies are the first to show that the absence of Tim-3/gal-9 pathway interactions augments systemic graft versus host disease and regulatory T-cells contribute to it. PMID: 22677125
  27. We concluded that pro-apoptotic effects on Th1 population through galectin-9-Tim-3 pathway and the up-regulation of Tim-2 on Th2 cells might be critical to Th2-biased response of host with schistosomiasis japonica. PMID: 22469568
  28. Gal-9 negatively regulates Th2-mediated eosinophilic inflammation of the lung and Foxp3+ regulatory T cells might be involved in suppressing allergic inflammation PMID: 22627368
  29. Data show exacerbated inflammation and increased IL-17-producing Th17 cells balanced by a decrease in Foxp3+ Tregs in Gal-9(-/-) experimental allergic encephalomyelitis model. PMID: 22341088
  30. host homeostatic mechanism mediated by Gal-9/Tim-3 interaction on CD8(+) T cells can influence the outcome of HSV-1 latent infection. PMID: 22021615
  31. In a mouse food allergy model, blocking Gal-9 inhibited the allergic hypersensitivity status and the antigen-specific Th2 response in the intestine. PMID: 21426359
  32. Galectin-9 and Tim-3 are closely related to inflammatory process in asthma. PMID: 21575348
  33. The N- and C-terminal CRDs of Gal-9 contribute differently to its ability to induce T cell death and to activate dendritic cells. PMID: 21146220
  34. the T(H)1 cell surface molecule Tim3 has evolved to inhibit growth of intracellular pathogens via its ligand Gal9, which in turn inhibits expansion of effector T(H)1 cells to prevent further tissue inflammation. PMID: 20937702
  35. galectin (Gal)-9 acts to constrain CD8(+) T cell immunity to Herpes Simplex Virus (HSV) infection PMID: 20463811
  36. elevated protein level in the lungs of mice with of ovalbumin-induced allergic asthma PMID: 19851072
  37. the Tim-3-galectin-9 pathway may have evolved to ensure effective termination of effector T helper type 1 cells PMID: 16286920
  38. analysis of the crystal structure of the galectin-9 N-terminal carbohydrate recognition domain PMID: 16990264
  39. Structural analysis of LGALS9 terminal carbohydrate recognition domain reveals unexpected properties that differ from the mouse orthologue, Lgals9. PMID: 18005988
  40. galectin-9 ameliorates mouse collagen-induced arthritis by suppressing the generation of Th17, promoting the induction of regulatory T cells. PMID: 18282810
  41. Tim-3-Galectin-9 pathway plays an important role in the termination of productive Th1-immune response and could lead to developing novel therapies in transplant medicine. PMID: 18346632
  42. Gal-9 suppresses both attachment and invasion of tumor cells by inhibiting the binding of adhesive molecules on tumor cells to ligands on vascular endothelium and ECM. PMID: 18579572
  43. Gal-9 promotes natural killer cell-mediated anti-tumor activity by expanding unique macrophages with a plasmacytoid dendritic cell-like phenotype PMID: 18974023
  44. Gal-9 potentiates CD8(+) T cell-mediated antitumor immunity via Gal-9-Tim-3 interactions between DCs and CD8(+) T cells. PMID: 19017954
  45. The T cell immunoglobulin and mucin-3 (TIM-3)/galectin-9 interaction plays a critical role in influencing the expression of herpes simplex virus-induced ocular lesions. PMID: 19234217
  46. expressed on CD4(+)CD25(+) regulatory T regs involved in suppression of skin graft alloresponse PMID: 19362679
  47. gal-9 may downregulate Th1 immune response in NOD mice and could be used as a therapeutic target in autoimmune diabetes. PMID: 19670381
  48. Galectin-9 is a high affinity IgE-binding lectin with anti-allergic effect by blocking IgE-antigen complex formation PMID: 19776007
  49. Galectin 9 upregulates FcgammaRiib and down-regulates FcgammaRiii expression in macrophages and thus suppresses proinflammatory cytokine production and C5a generation. PMID: 19800850

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Subcellular Location Cytoplasm, Nucleus, Secreted
Tissue Specificity Accentuated expression in liver and thymus of embryo, detected in embryonic heart, brain, lung, liver, and kidney. Highly expressed in adult thymus, small intestine, and liver, and to a lesser extent in lung, kidney, spleen, cardiac, and skeletal muscle.
Database Links

KEGG: mmu:16859

STRING: 10090.ENSMUSP00000103904

UniGene: Mm.341434

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