Mouse ICOS ligand(ICOSLG) ELISA kit

Instructions
Code CSB-EL010958MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name inducible T-cell co-stimulator ligand
Alternative Names Icoslg ELISA Kit; B7h2 ELISA Kit; B7rp1 ELISA Kit; IcoslICOS ligand ELISA Kit; B7 homolog 2 ELISA Kit; B7-H2 ELISA Kit; B7-like protein Gl50 ELISA Kit; B7-related protein 1 ELISA Kit; B7RP-1 ELISA Kit; LICOS ELISA Kit; CD antigen CD275 ELISA Kit
Abbreviation ICOSLG
Uniprot No. Q9JHJ8
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ICOSLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %88-97
1:2Average %100
Range %95-104
1:4Average %97
Range %92-110
1:8Average %89
Range %84-93
Recovery
The recovery of mouse ICOSLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-94
EDTA plasma (n=4)9287-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.417 2.552 2.485 2.322
20002.104 2.076 2.090 1.927
10001.450 1.404 1.427 1.264
5000.765 0.723 0.744 0.581
2500.439 0.471 0.455 0.292
1250.321 0.342 0.332 0.169
62.50.243 0.229 0.236 0.073
00.169 0.157 0.163
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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 Q&A
Q:

Did you test whether the ELISA kit detects recombinant mouse ICOSL protein, a positive control?
The reason we're asking you this because two kits we bought from 2 different companies did not capture the recombinant protein (which is very weird) but only their own standards.
Have already tested this binding capability of the kit?

A:
Thanks for your inquiry!
CSB-EL010958MO
This kit is designed accoding to: https://www.uniprot.org/uniprot/Q9JHJ8.
The standard is 293 cell derived recombinant protein.
“recombinant mouse ICOSL protein”you mentioned should be recombinant mouse ICOSLG protein mentioned in the above link. Sorry we are not clear about the elisa kit from other suppliers, but we could provide you with antibody information about this kit.
Capture antibody is rat monoclonal antibody and detection antibody is goat polyclonal antibody.
In principle, if the structure of recombinant protein is consistent with that of native protein, it can be detected, but in many cases the expression system used for recombinant protein will be different, so the later expression quality and modifications, etc are different. What's more, it also involves antibody's recognition area. If the expression area of recombinant protein is just in the antibody's recognition area, in principle it can be detected. However, the protein is basically in the presence of spatial structure, and it is the same for recombinant protein. During the process of translation and modification of recombinant proteins at the later stage of expression, there will be alpha helix and folding, forming spatial structure.
So whether it can be detected or not depends on the results of the experiment.
Pls let me know if you have any further questions. Thank you.

Target Data

Function Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.
Gene References into Functions
  1. study confirms the importance of ICOSL shedding in ICOS/ICOSL function and expression and it identifies ADAM10 as the most important sheddase for controlling ICOSL levels PMID: 28814605
  2. results showed that monocyte-derived osteoclast (OC)-like cells (MDOCs) express B7h during their differentiation, and that B7h triggering reversibly inhibits OC differentiation and function both in vitro and in vivo PMID: 27798154
  3. study concludse that lymphopenia-driven autoimmunity and the development of Tfh17 cells in Rasgrp1-deficient mice occur independently of CD275(B7-H2) PMID: 27183569
  4. The expression of ICOSL in the cornea and the ICOS-mediated induction of Foxp3+ CD4+ regulatory T cells may contribute to successful corneal allograft survival. PMID: 28002569
  5. this study shows that sICOSL in the serum of healthy donors increases in an age-dependent manner and that the matrix metalloproteinase inhibitor (MMPI) could suppress sICOSL production PMID: 27138044
  6. There was a clearly positive correlation between the presence of IL-17-producing cells and ICOS expression in ICOSL KO mice; it also showed that Th17 was involved in the pathological tissue remodeling in liver fibrosis induced by schistosomiasis. PMID: 25590646
  7. Selective ablation of ICOSL in CD11c+ cells, but not in B cells, dramatically ameliorates kidney and lung inflammation in lupus-prone transgenic mice. PMID: 25786178
  8. restoring control of the T follicular helper-germinal center B-cell axis by blocking the ICOS-ICOSL pathway reduced the development of atherosclerosis and the formation of tertiary lymphoid organs. PMID: 25552357
  9. ICOSL is a molecular linkage between T-B interactional dynamics and positive selection for high-affinity bone-marrow plasma cell formation; study reveals a pathway by which follicular T-helper cells control the quality of long-lived humoral immunity PMID: 25317561
  10. the costimulatory ligand ICOS ligand (ICOSL) is selectively downregulated on the surface of B cells in an ADAM17-specific manner, although it is not proteolitically processed by recombinant ADAM17 in vitro. PMID: 25108021
  11. the B7h-ICOS interaction may modulate the spread of cancer metastases PMID: 24729612
  12. Findings indicate the separable roles of delivery of antigens and ICOS-L by cognate B cells for follicular Th (Tfh) cell maturation and function. PMID: 24610013
  13. Data indicate that the signals provided by ICOSL-expressing B cells to Teff cells and Tfh cells are necessary for the development of arthritis. PMID: 24449576
  14. ICOS-L maintains tolerance at the fetomaternal interface. PMID: 23578385
  15. In cardiac transplantation apoptosis of CD8(+) T cells in recipient draining lymph nodes was enhanced by pretreatment with donor specific transfusion and impaired ICOS/B7h allorecognition. PMID: 23498793
  16. Data suggest that knockdown of Icosl in mouse leukemic cells may significantly enhance graft versus leukemia effect after allogeneic bone marrow transplantation. PMID: 22732700
  17. lineage-specific transgenic expression on dendritic and plasma cells generates different outcome upon ICOS costimulation PMID: 22686515
  18. Results indicate that ICOSLG blockade may enhance cytotoxity in allogeneic mixed lymphocyte-hematologic neoplasm cell reactions. PMID: 21958057
  19. Induction of IL-10 producing regulatory T cells mainly relied on the inducible costimulator ligand (ICOSL)/ICOS axis PMID: 22154192
  20. ICOS/B7h signal plays an important role in direct allorecognition, eliciting allogeneic responses in vitro. PMID: 22172879
  21. Findings suggest that the noncanonical NF-kappaB pathway regulates the development of Tfh cells by mediating ICOSL gene expression in B cells. PMID: 21768353
  22. A role for ICOS costimulation in the maintenance of effector memory but not central memory CD4 T cells. PMID: 21364749
  23. demonstrate that inducible costimulator (ICOS) provides a critical early signal to induce the transcription factor Bcl6, and Bcl6 then induces CXCR5, the canonical feature of follicular helper CD4(+) T cells PMID: 21636296
  24. expression is essential for allergic airway hyperresponsiveness PMID: 21402623
  25. The data suggest that ICOSL plays a significant role in immunoregulation and protective immunity against Chlamydia infections PMID: 20190137
  26. These studies demonstrate that T(R) cells and the ICOS-ICOS-ligand signaling pathway are critically involved in respiratory tolerance and in downregulating pulmonary inflammation in asthma. PMID: 12145647
  27. The involvement of the B7h-Icos costimulatory pathway has been demonstrated in the development and progression of collagen-induced arthritis. PMID: 12370365
  28. ICOS-L is a ligand for ICOS and plays an important functional role in the activation of memory T cells by endothelial cells [review] PMID: 12456022
  29. While interactions of B7RP-1 with ICOS have no effect on the migration of T cells into B cell follicles, they are required for Th1 and Th2 cells to support fulminant B cell responses in vivo. PMID: 12594252
  30. B7-H2 on B cells is important for recruiting T-cell help via its interaction with ICOS and plays a critical role in costimulating humoral immune responses. PMID: 12714510
  31. data suggest that ICOS/B7RP-1 interactions may not affect the organogenesis, but involve in the functional development of Peyer's patches PMID: 12853164
  32. the ICOS-B7h pathway has roles in regulating alloimmune responses in vivo PMID: 12865411
  33. Th cells, when activated with B7h-deficient APC, exhibited reduced proliferation and IL-2 production. PMID: 14615582
  34. ICOS-B7h has a role in transplantation tolerance to MHC class II mismatched skin grafts PMID: 15636609
  35. Experiments with mice genetically engineered for the absence of B7-related protein 1 (B7RP-1KO) demonstrate that efficient T helper 2 (TH2) cell sensitization and effector function occur in the absence of the ICOS-B7RP-1 costimulatory pathway. PMID: 15728513
  36. ICOS-B7h pathway is critical in the activation of effector/memory T cells that are necessary for the progression of chronic rejection. PMID: 15880041
  37. The B7RP-1/ICOS interaction plays an essential role in the development of CXCR-positive helper T cells in vivo, which preferentially migrate to the germinal center B cell zone where they provide cognate help to B cells. PMID: 16081804
  38. ICOSL is upregulated in nephritic glomeruli, where it locally reduces accumulation of T cells and macrophages and attenuates renal injury. PMID: 16540559
  39. critical role played by ICOS-L-expressing and interleukin 10-producing dendritic cells from Chlamydia-infected mice in the infection-mediated inhibition of allergic responses PMID: 16621988
  40. B7h shedding defines a novel immunoregulatory mechanism by which toll-like receptor (TLR)7/8 and TLR9 signals can enhance T cell help during antibody responses. PMID: 16887997
  41. These results suggest that the ICOS/B7RP-1 interaction plays a critical role in the pathogenesis of uveitis. PMID: 17039566
  42. The generated a 3T3 cellular library retrovirally expressing mutants of the murine costimulatory B7h gene. Screening of this unbiased cellular library identified six residues of murine B7h that are critical for binding to the ICOS receptor. PMID: 18294651
  43. a novel mechanism for the regulation of ICOSL expression in vivo and suggest that the ICOS costimulatory pathway is subject to negative feedback regulation by ICOSL down-regulation in response to ICOS expression. PMID: 18390703
  44. Levels of follicular T helper cells and germinal center B cells in two different models of autoimmunity are shown to be dependent on the maintenance of the inducible T-cell co-stimulator (ICOS)/B7-related protein-1 (B7RP-1) pathway. PMID: 19155489
  45. These findings implicate ICOSL-induced IL-10, but not IDO in the regulation of BM-derived pDC function. PMID: 19208362
  46. compared T cell responses of WT, CD28 knockout (KO), B7-H2 KO, and CD28-B7-H2 double KO (DKO) mice in a model of delayed-type hypersensitivity PMID: 19249753
  47. This molecule acts as a costimulatory counterreceptor by augmenting the recall responses of CD8+ T cells. PMID: 11418641

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Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Immunoglobulin superfamily, BTN/MOG family
Tissue Specificity Isoform 1 highest expression in lymphoid tissues, such as spleen (mostly in the marginal zone), lymph nodes (particularly in the cortex and in both primary and secondary follicles), thymus (predominantly in the medulla) and Peyer patches (mostly in the fo
Database Links

KEGG: mmu:50723

STRING: 10090.ENSMUSP00000101032

UniGene: Mm.17819

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